First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen
Abstract Late blight, caused by Phytophthora infestans, is one of the most destructive potato diseases in the world. In Yemen, identification of P. infestans still depends on a visual survey and external examination of late blight symptoms. The objective of this study was to isolate and identify P....
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-43510-2 |
| _version_ | 1797453256646983680 |
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| author | Amira A. Al Harethi Qais Y. M. Abdullah Hala J. Al Jobory Samar A. Al Aquil Ramadan A. Arafa |
| author_facet | Amira A. Al Harethi Qais Y. M. Abdullah Hala J. Al Jobory Samar A. Al Aquil Ramadan A. Arafa |
| author_sort | Amira A. Al Harethi |
| collection | DOAJ |
| description | Abstract Late blight, caused by Phytophthora infestans, is one of the most destructive potato diseases in the world. In Yemen, identification of P. infestans still depends on a visual survey and external examination of late blight symptoms. The objective of this study was to isolate and identify P. infestans by using advanced methods. We collected 71 disease samples and isolated the pathogen using the tuber slice method. To identify an isolated pathogen, we performed morphological characterization and gene sequence analysis of the coding genes for internal transcribed spacers. We used Koch’s hypotheses to confirm the previous results. In our study. The morphological characters of the mycelium pattern of P. infestans isolates in Yemen were profusely branching, fluffy, and white. The sporangia showed remarkable limoniform papillate sporangial shape. with average length and width of 30.6 and 28.6 µm, respectively. The sequences analysis showed high homology with a degree of identity ranging from 98 to 100% to the database sequences on GenBank. Pathogenicity tests showed that the P. infestans was the causal agent. To our knowledge, this is the first study of the isolation and characterization of P. infestans in Yemen. |
| first_indexed | 2024-03-09T15:20:12Z |
| format | Article |
| id | doaj.art-1689a567584c4d018ff0d17d8f02626d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:20:12Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1689a567584c4d018ff0d17d8f02626d2023-11-26T12:51:05ZengNature PortfolioScientific Reports2045-23222023-09-011311910.1038/s41598-023-43510-2First report of molecular identification of Phytophthora infestans causing potato late blight in YemenAmira A. Al Harethi0Qais Y. M. Abdullah1Hala J. Al Jobory2Samar A. Al Aquil3Ramadan A. Arafa4Department of Biological Science, Faculty of Science, Sana’a UniversityDepartment of Biological Science, Faculty of Science, Sana’a UniversityDepartment of Biological Science, Faculty of Science, Sana’a UniversityDepartment of Physics, Faculty of Science, Sana’a UniversityPlant Pathology Research Institute, Agricultural Research CenterAbstract Late blight, caused by Phytophthora infestans, is one of the most destructive potato diseases in the world. In Yemen, identification of P. infestans still depends on a visual survey and external examination of late blight symptoms. The objective of this study was to isolate and identify P. infestans by using advanced methods. We collected 71 disease samples and isolated the pathogen using the tuber slice method. To identify an isolated pathogen, we performed morphological characterization and gene sequence analysis of the coding genes for internal transcribed spacers. We used Koch’s hypotheses to confirm the previous results. In our study. The morphological characters of the mycelium pattern of P. infestans isolates in Yemen were profusely branching, fluffy, and white. The sporangia showed remarkable limoniform papillate sporangial shape. with average length and width of 30.6 and 28.6 µm, respectively. The sequences analysis showed high homology with a degree of identity ranging from 98 to 100% to the database sequences on GenBank. Pathogenicity tests showed that the P. infestans was the causal agent. To our knowledge, this is the first study of the isolation and characterization of P. infestans in Yemen.https://doi.org/10.1038/s41598-023-43510-2 |
| spellingShingle | Amira A. Al Harethi Qais Y. M. Abdullah Hala J. Al Jobory Samar A. Al Aquil Ramadan A. Arafa First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen Scientific Reports |
| title | First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen |
| title_full | First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen |
| title_fullStr | First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen |
| title_full_unstemmed | First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen |
| title_short | First report of molecular identification of Phytophthora infestans causing potato late blight in Yemen |
| title_sort | first report of molecular identification of phytophthora infestans causing potato late blight in yemen |
| url | https://doi.org/10.1038/s41598-023-43510-2 |
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