Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC
<i>Uraria crinita</i> is used as a functional food ingredient. Little is known about the association between its immunomodulatory activity and its metabolites. We applied a precise strategy for screening metabolites using immunomodulatory fractions from a <i>U. crinata</i> ro...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/8/11/543 |
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| author | Ping-Chen Tu Chih-Ju Chan Yi-Chen Liu Yueh-Hsiung Kuo Ming-Kuem Lin Meng-Shiou Lee |
| author_facet | Ping-Chen Tu Chih-Ju Chan Yi-Chen Liu Yueh-Hsiung Kuo Ming-Kuem Lin Meng-Shiou Lee |
| author_sort | Ping-Chen Tu |
| collection | DOAJ |
| description | <i>Uraria crinita</i> is used as a functional food ingredient. Little is known about the association between its immunomodulatory activity and its metabolites. We applied a precise strategy for screening metabolites using immunomodulatory fractions from a <i>U. crinata</i> root methanolic extract (UCME) in combination with bioactivity-guided fractionation and NMR-based identification. The fractions from UCME were evaluated in terms of their inhibitory activity against the production of pro-inflammatory cytokines (IL-6 and TNF-<i>α</i>) by lipopolysaccharide (LPS)-stimulated mouse bone marrow-derived dendritic cells (BMDC). The role of the isoflavone genistein was indicated by the <sup>1</sup>H NMR profiling of immunomodulatory subfractions (D-4 and D-5) and supported by the result that genistein-knockout subfractions (D-4 <i>w</i>/<i>o</i> and D-5 <i>w</i>/<i>o</i>) had a lower inhibitory activity compared to genistein-containing subfractions. This study suggests that genistein contributes to the immunomodulatory activity of UCME and will help in the standardization of functional food. |
| first_indexed | 2024-04-13T11:34:48Z |
| format | Article |
| id | doaj.art-168a310ef5f5456aafb5a8370d2497a1 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-13T11:34:48Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-168a310ef5f5456aafb5a8370d2497a12022-12-22T02:48:28ZengMDPI AGFoods2304-81582019-11-0181154310.3390/foods8110543foods8110543Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DCPing-Chen Tu0Chih-Ju Chan1Yi-Chen Liu2Yueh-Hsiung Kuo3Ming-Kuem Lin4Meng-Shiou Lee5Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung 404, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, TaiwanInstitute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, Taichung 402, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan<i>Uraria crinita</i> is used as a functional food ingredient. Little is known about the association between its immunomodulatory activity and its metabolites. We applied a precise strategy for screening metabolites using immunomodulatory fractions from a <i>U. crinata</i> root methanolic extract (UCME) in combination with bioactivity-guided fractionation and NMR-based identification. The fractions from UCME were evaluated in terms of their inhibitory activity against the production of pro-inflammatory cytokines (IL-6 and TNF-<i>α</i>) by lipopolysaccharide (LPS)-stimulated mouse bone marrow-derived dendritic cells (BMDC). The role of the isoflavone genistein was indicated by the <sup>1</sup>H NMR profiling of immunomodulatory subfractions (D-4 and D-5) and supported by the result that genistein-knockout subfractions (D-4 <i>w</i>/<i>o</i> and D-5 <i>w</i>/<i>o</i>) had a lower inhibitory activity compared to genistein-containing subfractions. This study suggests that genistein contributes to the immunomodulatory activity of UCME and will help in the standardization of functional food.https://www.mdpi.com/2304-8158/8/11/543<i>uraria crinita</i>isoflavonegenisteinnmr-based identificationdendritic cells |
| spellingShingle | Ping-Chen Tu Chih-Ju Chan Yi-Chen Liu Yueh-Hsiung Kuo Ming-Kuem Lin Meng-Shiou Lee Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC Foods <i>uraria crinita</i> isoflavone genistein nmr-based identification dendritic cells |
| title | Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC |
| title_full | Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC |
| title_fullStr | Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC |
| title_full_unstemmed | Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC |
| title_short | Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of <i>Uraria crinita</i> (L.) Desv. ex DC |
| title_sort | bioactivity guided fractionation and nmr based identification of the immunomodulatory isoflavone from the roots of i uraria crinita i l desv ex dc |
| topic | <i>uraria crinita</i> isoflavone genistein nmr-based identification dendritic cells |
| url | https://www.mdpi.com/2304-8158/8/11/543 |
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