ObjectiveThe aim of this study was to produce dense granules of tricalcium phosphate (β-TCP) and magnesium (Mg) substituted β-TCP, also known as β-TCMP (Mg/Ca=0.15 mol), in order to evaluate the impact of Mg incorporation on the physicochemical parameters and in vitro bioc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of São Paulo
2013-03-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000100037 |
| _version_ | 1818451014173327360 |
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| author | Débora dos Santos TAVARES Leticia de Oliveira CASTRO Gloria Dulce de Almeida SOARES Gutemberg Gomes ALVES José Mauro GRANJEIRO |
| author_facet | Débora dos Santos TAVARES Leticia de Oliveira CASTRO Gloria Dulce de Almeida SOARES Gutemberg Gomes ALVES José Mauro GRANJEIRO |
| author_sort | Débora dos Santos TAVARES |
| collection | DOAJ |
| description | ObjectiveThe aim of this study was to produce dense granules of tricalcium phosphate (β-TCP) and magnesium (Mg) substituted β-TCP, also known as β-TCMP (Mg/Ca=0.15 mol), in order to evaluate the impact of Mg incorporation on the physicochemical parameters and in vitro biocompatibility of this novel material.Material and MethodsThe materials were characterized using X-ray diffraction (XRD), infrared spectroscopy (FTIR), electron microscopy and inductively coupled plasma (ICP). Biocompatibility was assayed according to ISO 10993-12:2007 and 7405:2008, by two different tests of cell survival and integrity (XTT and CVDE).ResultsThe XRD profile presented the main peaks of β-TCP (JCPDS 090169) and β-TCMP (JCPDS 130404). The characteristic absorption bands of TCP were also identified by FTIR. The ICP results of β-TCMP granules extract showed a precipitation of calcium and release of Mg into the culture medium. Regarding the cytotoxicity assays, β-TCMP dense granules did not significantly affect the mitochondrial activity and relative cell density in relation to β-TCP dense granules, despite the release of Mg from granules into the cell culture medium.Conclusionβ-TCMP granules were successfully produced and were able to release Mg into media without cytotoxicity, indicating the suitability of this promising material for further biological studies on its adequacy for bone therapy. |
| first_indexed | 2024-12-14T21:00:27Z |
| format | Article |
| id | doaj.art-168c6d945d404d3094ddeaf824589d15 |
| institution | Directory Open Access Journal |
| issn | 1678-7757 1678-7765 |
| language | English |
| last_indexed | 2024-12-14T21:00:27Z |
| publishDate | 2013-03-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-168c6d945d404d3094ddeaf824589d152022-12-21T22:47:35ZengUniversity of São PauloJournal of Applied Oral Science1678-77571678-77652013-03-012113742Débora dos Santos TAVARESLeticia de Oliveira CASTROGloria Dulce de Almeida SOARESGutemberg Gomes ALVESJosé Mauro GRANJEIROObjectiveThe aim of this study was to produce dense granules of tricalcium phosphate (β-TCP) and magnesium (Mg) substituted β-TCP, also known as β-TCMP (Mg/Ca=0.15 mol), in order to evaluate the impact of Mg incorporation on the physicochemical parameters and in vitro biocompatibility of this novel material.Material and MethodsThe materials were characterized using X-ray diffraction (XRD), infrared spectroscopy (FTIR), electron microscopy and inductively coupled plasma (ICP). Biocompatibility was assayed according to ISO 10993-12:2007 and 7405:2008, by two different tests of cell survival and integrity (XTT and CVDE).ResultsThe XRD profile presented the main peaks of β-TCP (JCPDS 090169) and β-TCMP (JCPDS 130404). The characteristic absorption bands of TCP were also identified by FTIR. The ICP results of β-TCMP granules extract showed a precipitation of calcium and release of Mg into the culture medium. Regarding the cytotoxicity assays, β-TCMP dense granules did not significantly affect the mitochondrial activity and relative cell density in relation to β-TCP dense granules, despite the release of Mg from granules into the cell culture medium.Conclusionβ-TCMP granules were successfully produced and were able to release Mg into media without cytotoxicity, indicating the suitability of this promising material for further biological studies on its adequacy for bone therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000100037MagnesiumBone substitutesBiocompatibility |
| spellingShingle | Débora dos Santos TAVARES Leticia de Oliveira CASTRO Gloria Dulce de Almeida SOARES Gutemberg Gomes ALVES José Mauro GRANJEIRO Journal of Applied Oral Science Magnesium Bone substitutes Biocompatibility |
| topic | Magnesium Bone substitutes Biocompatibility |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572013000100037 |