New synchronization method for <it>Plasmodium falciparum</it>
<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>is usually asynchronous during <it>in vitro </it>culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A ne...
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| Format: | Article |
| Language: | English |
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BMC
2010-06-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/9/1/170 |
| _version_ | 1811284045375471616 |
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| author | Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C |
| author_facet | Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C |
| author_sort | Mwangi Jonathan M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>is usually asynchronous during <it>in vitro </it>culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cultures with an age range as low as 30 minutes.</p> <p>Methods</p> <p>Trophozoites and schizonts are enriched using Plasmion. The enriched late stage parasites are immobilized as a monolayer onto plastic Petri dishes using concanavalin A. Uninfected erythrocytes are placed onto the monolayer for a limited time period, during which time schizonts on the monolayer rupture and the released merozoites invade the fresh erythrocytes. The overlay is then taken off into a culture flask, resulting in a highly synchronized population of parasites.</p> <p>Results</p> <p>Plasmion treatment results in a 10- to 13-fold enrichment of late stage parasites. The monolayer method results in highly synchronized cultures of parasites where invasion has occurred within a very limited time window, which can be as low as 30 minutes. The method is simple, requiring no specialized equipment and relatively cheap reagents.</p> <p>Conclusions</p> <p>The new method for parasite synchronization results in highly synchronized populations of parasites, which will be useful for studies of the parasite asexual cell cycle.</p> |
| first_indexed | 2024-04-13T02:22:44Z |
| format | Article |
| id | doaj.art-168d5b0223cf4f7b93303c20d655f662 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-13T02:22:44Z |
| publishDate | 2010-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-168d5b0223cf4f7b93303c20d655f6622022-12-22T03:06:53ZengBMCMalaria Journal1475-28752010-06-019117010.1186/1475-2875-9-170New synchronization method for <it>Plasmodium falciparum</it>Mwangi Jonathan MHumphreys Georgina SSinha AbhinavRanford-Cartwright Lisa C<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>is usually asynchronous during <it>in vitro </it>culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cultures with an age range as low as 30 minutes.</p> <p>Methods</p> <p>Trophozoites and schizonts are enriched using Plasmion. The enriched late stage parasites are immobilized as a monolayer onto plastic Petri dishes using concanavalin A. Uninfected erythrocytes are placed onto the monolayer for a limited time period, during which time schizonts on the monolayer rupture and the released merozoites invade the fresh erythrocytes. The overlay is then taken off into a culture flask, resulting in a highly synchronized population of parasites.</p> <p>Results</p> <p>Plasmion treatment results in a 10- to 13-fold enrichment of late stage parasites. The monolayer method results in highly synchronized cultures of parasites where invasion has occurred within a very limited time window, which can be as low as 30 minutes. The method is simple, requiring no specialized equipment and relatively cheap reagents.</p> <p>Conclusions</p> <p>The new method for parasite synchronization results in highly synchronized populations of parasites, which will be useful for studies of the parasite asexual cell cycle.</p>http://www.malariajournal.com/content/9/1/170 |
| spellingShingle | Mwangi Jonathan M Humphreys Georgina S Sinha Abhinav Ranford-Cartwright Lisa C New synchronization method for <it>Plasmodium falciparum</it> Malaria Journal |
| title | New synchronization method for <it>Plasmodium falciparum</it> |
| title_full | New synchronization method for <it>Plasmodium falciparum</it> |
| title_fullStr | New synchronization method for <it>Plasmodium falciparum</it> |
| title_full_unstemmed | New synchronization method for <it>Plasmodium falciparum</it> |
| title_short | New synchronization method for <it>Plasmodium falciparum</it> |
| title_sort | new synchronization method for it plasmodium falciparum it |
| url | http://www.malariajournal.com/content/9/1/170 |
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