Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells
Abstract Obesity is a health condition accompanied by life-threatening comorbidities; hence, there is an increasing need for anti-obesity agents. The anti-cancer effects of the leaves of Asimina triloba (pawpaw) has been reported. However, limited research has been conducted on the potential anti-ob...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-11-01
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| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13765-023-00837-8 |
| _version_ | 1797636664880791552 |
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| author | Chang Jun Lee Young-Soo Kim Jinyoung Hur Guijae Yoo Sang Yoon Choi |
| author_facet | Chang Jun Lee Young-Soo Kim Jinyoung Hur Guijae Yoo Sang Yoon Choi |
| author_sort | Chang Jun Lee |
| collection | DOAJ |
| description | Abstract Obesity is a health condition accompanied by life-threatening comorbidities; hence, there is an increasing need for anti-obesity agents. The anti-cancer effects of the leaves of Asimina triloba (pawpaw) has been reported. However, limited research has been conducted on the potential anti-obesity effects of A. triloba fruit. Therefore, this study aimed to explore the effects of A. triloba fruit extract on murine preadipocytes (3T3-L1). We specifically examined lipid droplet formation in these cells using Oil Red O solution and intracellular pro-adipogenic protein levels were examined using western blot analysis. The results revealed that treatment with A. triloba 70% ethanolic fruit extract effectively suppressed lipid droplet formation. Moreover, the expression of crucial proteins involved in adipogenesis, namely sterol regulatory element-binding protein 1, peroxisome proliferator-activated receptor γ, and fatty acid synthase, were significantly inhibited. These findings suggest that A. triloba fruit has the potential to prevent obesity by inhibiting fat synthesis and may serve as a natural source for anti-obesity functional agents. . |
| first_indexed | 2024-03-11T12:38:20Z |
| format | Article |
| id | doaj.art-1690134c09274a5281f499423e120643 |
| institution | Directory Open Access Journal |
| issn | 2468-0842 |
| language | English |
| last_indexed | 2024-03-11T12:38:20Z |
| publishDate | 2023-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-1690134c09274a5281f499423e1206432023-11-05T12:27:28ZengSpringerOpenApplied Biological Chemistry2468-08422023-11-016611910.1186/s13765-023-00837-8Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cellsChang Jun Lee0Young-Soo Kim1Jinyoung Hur2Guijae Yoo3Sang Yoon Choi4Korea Food Research InstituteDepartment of Food Science and Technology, Jeonbuk National UniversityKorea Food Research InstituteKorea Food Research InstituteKorea Food Research InstituteAbstract Obesity is a health condition accompanied by life-threatening comorbidities; hence, there is an increasing need for anti-obesity agents. The anti-cancer effects of the leaves of Asimina triloba (pawpaw) has been reported. However, limited research has been conducted on the potential anti-obesity effects of A. triloba fruit. Therefore, this study aimed to explore the effects of A. triloba fruit extract on murine preadipocytes (3T3-L1). We specifically examined lipid droplet formation in these cells using Oil Red O solution and intracellular pro-adipogenic protein levels were examined using western blot analysis. The results revealed that treatment with A. triloba 70% ethanolic fruit extract effectively suppressed lipid droplet formation. Moreover, the expression of crucial proteins involved in adipogenesis, namely sterol regulatory element-binding protein 1, peroxisome proliferator-activated receptor γ, and fatty acid synthase, were significantly inhibited. These findings suggest that A. triloba fruit has the potential to prevent obesity by inhibiting fat synthesis and may serve as a natural source for anti-obesity functional agents. .https://doi.org/10.1186/s13765-023-00837-8Asimina trilobaPawpaw3T3-L1 cellObesityLipogenesis |
| spellingShingle | Chang Jun Lee Young-Soo Kim Jinyoung Hur Guijae Yoo Sang Yoon Choi Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells Applied Biological Chemistry Asimina triloba Pawpaw 3T3-L1 cell Obesity Lipogenesis |
| title | Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells |
| title_full | Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells |
| title_fullStr | Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells |
| title_full_unstemmed | Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells |
| title_short | Asimina triloba (pawpaw) fruit extract suppresses adipocyte differentiation and lipogenesis-related protein expression in 3T3-L1 cells |
| title_sort | asimina triloba pawpaw fruit extract suppresses adipocyte differentiation and lipogenesis related protein expression in 3t3 l1 cells |
| topic | Asimina triloba Pawpaw 3T3-L1 cell Obesity Lipogenesis |
| url | https://doi.org/10.1186/s13765-023-00837-8 |
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