Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy
With the aim improving drug delivery, liposomes have been employed as carriers for chemotherapeutics achieving promising results; their co-encapsulation with magnetic nanoparticles is evaluated in this work. The objective of this study was to examine the physicochemical characteristics, the pharmaco...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/9/2272 |
| _version_ | 1817984530827444224 |
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| author | Alfonso Toro-Cordova Mario Flores-Cruz Jaime Santoyo-Salazar Ernesto Carrillo-Nava Rafael Jurado Pavel A. Figueroa-Rodriguez Pedro Lopez-Sanchez Luis A. Medina Patricia Garcia-Lopez |
| author_facet | Alfonso Toro-Cordova Mario Flores-Cruz Jaime Santoyo-Salazar Ernesto Carrillo-Nava Rafael Jurado Pavel A. Figueroa-Rodriguez Pedro Lopez-Sanchez Luis A. Medina Patricia Garcia-Lopez |
| author_sort | Alfonso Toro-Cordova |
| collection | DOAJ |
| description | With the aim improving drug delivery, liposomes have been employed as carriers for chemotherapeutics achieving promising results; their co-encapsulation with magnetic nanoparticles is evaluated in this work. The objective of this study was to examine the physicochemical characteristics, the pharmacokinetic behaviour, and the efficacy of pegylated liposomes loaded with cisplatin and magnetic nanoparticles (magnetite) (Cis-MLs). Cis-MLs were prepared by a modified reverse-phase evaporation method. To characterize their physicochemical properties, an evaluation was made of particle size, ζ-potential, phospholipid and cholesterol concentration, phase transition temperature (Tm), the encapsulation efficiency of cisplatin and magnetite, and drug release profiles. Additionally, pharmacokinetic studies were conducted on normal Wistar rats, while apoptosis and the cytotoxic effect were assessed with HeLa cells. We present a method for simultaneously encapsulating cisplatin at the core and also embedding magnetite nanoparticles on the membrane of liposomes with a mean vesicular size of 104.4 ± 11.5 nm and a ζ-potential of −40.5 ± 0.8 mV, affording a stable formulation with a safe pharmacokinetic profile. These liposomes elicited a significant effect on cell viability and triggered apoptosis in HeLa cells. |
| first_indexed | 2024-04-13T23:46:51Z |
| format | Article |
| id | doaj.art-16906d84a00d4b91af272270250b92b3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T23:46:51Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-16906d84a00d4b91af272270250b92b32022-12-22T02:24:17ZengMDPI AGMolecules1420-30492018-09-01239227210.3390/molecules23092272molecules23092272Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro EfficacyAlfonso Toro-Cordova0Mario Flores-Cruz1Jaime Santoyo-Salazar2Ernesto Carrillo-Nava3Rafael Jurado4Pavel A. Figueroa-Rodriguez5Pedro Lopez-Sanchez6Luis A. Medina7Patricia Garcia-Lopez8Laboratorio de Farmacología, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, 14080 CDMX, MexicoLaboratorio de Farmacología, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, 14080 CDMX, MexicoDepartamento de Física, Centro de Investigacion y de Estudios Avanzados del Instituto Politécnico Nacional, CINVESTAV-IPN, Zacatenco, 07360 CDMX, MexicoLaboratorio de Biofisicoquímica, Departamento de Fisicoquímica, Facultad de Química, Universidad Nacional Autónoma de México, 014510 CDMX, MexicoLaboratorio de Farmacología, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, 14080 CDMX, MexicoUnidad de Investigación Biomédica en Cáncer INCan-UNAM, Instituto Nacional de Cancerología, 14080 CDMX, MexicoSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, 11340 CDMX, MexicoUnidad de Investigación Biomédica en Cáncer INCan-UNAM, Instituto Nacional de Cancerología, 14080 CDMX, MexicoLaboratorio de Farmacología, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, 14080 CDMX, MexicoWith the aim improving drug delivery, liposomes have been employed as carriers for chemotherapeutics achieving promising results; their co-encapsulation with magnetic nanoparticles is evaluated in this work. The objective of this study was to examine the physicochemical characteristics, the pharmacokinetic behaviour, and the efficacy of pegylated liposomes loaded with cisplatin and magnetic nanoparticles (magnetite) (Cis-MLs). Cis-MLs were prepared by a modified reverse-phase evaporation method. To characterize their physicochemical properties, an evaluation was made of particle size, ζ-potential, phospholipid and cholesterol concentration, phase transition temperature (Tm), the encapsulation efficiency of cisplatin and magnetite, and drug release profiles. Additionally, pharmacokinetic studies were conducted on normal Wistar rats, while apoptosis and the cytotoxic effect were assessed with HeLa cells. We present a method for simultaneously encapsulating cisplatin at the core and also embedding magnetite nanoparticles on the membrane of liposomes with a mean vesicular size of 104.4 ± 11.5 nm and a ζ-potential of −40.5 ± 0.8 mV, affording a stable formulation with a safe pharmacokinetic profile. These liposomes elicited a significant effect on cell viability and triggered apoptosis in HeLa cells.http://www.mdpi.com/1420-3049/23/9/2272cancercisplatindrug deliverymagnetiteliposomes pharmacokinetics |
| spellingShingle | Alfonso Toro-Cordova Mario Flores-Cruz Jaime Santoyo-Salazar Ernesto Carrillo-Nava Rafael Jurado Pavel A. Figueroa-Rodriguez Pedro Lopez-Sanchez Luis A. Medina Patricia Garcia-Lopez Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy Molecules cancer cisplatin drug delivery magnetite liposomes pharmacokinetics |
| title | Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy |
| title_full | Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy |
| title_fullStr | Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy |
| title_full_unstemmed | Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy |
| title_short | Liposomes Loaded with Cisplatin and Magnetic Nanoparticles: Physicochemical Characterization, Pharmacokinetics, and In-Vitro Efficacy |
| title_sort | liposomes loaded with cisplatin and magnetic nanoparticles physicochemical characterization pharmacokinetics and in vitro efficacy |
| topic | cancer cisplatin drug delivery magnetite liposomes pharmacokinetics |
| url | http://www.mdpi.com/1420-3049/23/9/2272 |
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