Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model
In this study, collagen hydrolysates (CHDs) were fabricated with honey-propolis wax (HPW), structurally modified as a sponge matrix, and experimentalized on wound healing in a mouse model. The scaffold was characterized by means of in vitro enzymatic degradation; in vitro HPW release; and in vivo wo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/20/7090 |
| _version_ | 1797470804711047168 |
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| author | Hairul-Islam Mohamed Ibrahim Muthukumar Thangavelu Ashraf Khalifa |
| author_facet | Hairul-Islam Mohamed Ibrahim Muthukumar Thangavelu Ashraf Khalifa |
| author_sort | Hairul-Islam Mohamed Ibrahim |
| collection | DOAJ |
| description | In this study, collagen hydrolysates (CHDs) were fabricated with honey-propolis wax (HPW), structurally modified as a sponge matrix, and experimentalized on wound healing in a mouse model. The scaffold was characterized by means of in vitro enzymatic degradation; in vitro HPW release; and in vivo wound-healing mouse model, wound-healing-specific RNA, transcripts, and protein markers. The functional activity of the HPW extracted from raw propolis was determined using total flavonoids, antioxidant scavenging assays, and anti-hemolytic principles. The results indicated that HPW had a high flavonoid content (20 μg/mL of wax) and antioxidant activities. The effective concentration (EC50) of HPW was estimated (28 mg/mL) and was then used in the subsequent in vivo experiments. Additionally, the dopped mixture of CHDs and HPW substantially enhanced the wound-healing process and regulated wound biochemical markers such as hexoseamine and melondialdehyde. CHDs- HPW upregulated the expression of growth factors including vascular endothelial growth factor (VEGF) (2.3-fold), fibroblast growth factor (FGF) and epidermal growth factor (EGF) (1.7-fold), and transforming growth factor-beta (TGF-β) (3.1-fold), indicating their potential capacity to perform wound re-epithelialization and the loading of ground tissue. Pro-inflammatory markers IL-1 β (51 pg/mL) and TNF-α (220 pg/mL) were significantly reduced in the CHD-HPW-treated wound. These interesting results were further confirmed using mRNA and protein growth factors from the wound, which enhanced the load of collagen-I in the wound site. In conclusion, CHDs-HPW exhibited a significant reduction in inflammation and inflammatory markers and helped to obtain a faster wound-healing process in a mouse model. The newly engineered biosponge could be developed as a promising therapeutic approach for the regeneration and repair of damaged human skin in the future. |
| first_indexed | 2024-03-09T19:41:07Z |
| format | Article |
| id | doaj.art-1694a31612cd4e92a6acf99dfe536738 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:41:07Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1694a31612cd4e92a6acf99dfe5367382023-11-24T01:37:12ZengMDPI AGMolecules1420-30492022-10-012720709010.3390/molecules27207090Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse ModelHairul-Islam Mohamed Ibrahim0Muthukumar Thangavelu1Ashraf Khalifa2Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaDepartment BIN Convergence Tech, Jeonbuk National University, 567 Baekje-dearo, Deokjin, Jeonju 54896, Jeollabuk-do, KoreaBiological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaIn this study, collagen hydrolysates (CHDs) were fabricated with honey-propolis wax (HPW), structurally modified as a sponge matrix, and experimentalized on wound healing in a mouse model. The scaffold was characterized by means of in vitro enzymatic degradation; in vitro HPW release; and in vivo wound-healing mouse model, wound-healing-specific RNA, transcripts, and protein markers. The functional activity of the HPW extracted from raw propolis was determined using total flavonoids, antioxidant scavenging assays, and anti-hemolytic principles. The results indicated that HPW had a high flavonoid content (20 μg/mL of wax) and antioxidant activities. The effective concentration (EC50) of HPW was estimated (28 mg/mL) and was then used in the subsequent in vivo experiments. Additionally, the dopped mixture of CHDs and HPW substantially enhanced the wound-healing process and regulated wound biochemical markers such as hexoseamine and melondialdehyde. CHDs- HPW upregulated the expression of growth factors including vascular endothelial growth factor (VEGF) (2.3-fold), fibroblast growth factor (FGF) and epidermal growth factor (EGF) (1.7-fold), and transforming growth factor-beta (TGF-β) (3.1-fold), indicating their potential capacity to perform wound re-epithelialization and the loading of ground tissue. Pro-inflammatory markers IL-1 β (51 pg/mL) and TNF-α (220 pg/mL) were significantly reduced in the CHD-HPW-treated wound. These interesting results were further confirmed using mRNA and protein growth factors from the wound, which enhanced the load of collagen-I in the wound site. In conclusion, CHDs-HPW exhibited a significant reduction in inflammation and inflammatory markers and helped to obtain a faster wound-healing process in a mouse model. The newly engineered biosponge could be developed as a promising therapeutic approach for the regeneration and repair of damaged human skin in the future.https://www.mdpi.com/1420-3049/27/20/7090collagenpropolisskinwound progressionVEGF |
| spellingShingle | Hairul-Islam Mohamed Ibrahim Muthukumar Thangavelu Ashraf Khalifa Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model Molecules collagen propolis skin wound progression VEGF |
| title | Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model |
| title_full | Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model |
| title_fullStr | Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model |
| title_full_unstemmed | Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model |
| title_short | Honey-Propolis-Engineered Collagen Peptides as Promising Wound-Healing Matrix in Mouse Model |
| title_sort | honey propolis engineered collagen peptides as promising wound healing matrix in mouse model |
| topic | collagen propolis skin wound progression VEGF |
| url | https://www.mdpi.com/1420-3049/27/20/7090 |
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