Transport stress affects the fecal microbiota in healthy donkeys
Abstract Background With the development of large‐scale donkey farming in China, long‐distance transportation has become common practice, and the incidence of intestinal diseases after transportation has increased. The intestinal microbiota is important in health and disease, and whether or not tran...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-09-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16235 |
| _version_ | 1819149092870160384 |
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| author | Guimiao Jiang Xinhao Zhang Weiping Gao Chuanliang Ji Yantao Wang Peixiang Feng Yulong Feng Zhiping Zhang Lin Li Fuwei Zhao |
| author_facet | Guimiao Jiang Xinhao Zhang Weiping Gao Chuanliang Ji Yantao Wang Peixiang Feng Yulong Feng Zhiping Zhang Lin Li Fuwei Zhao |
| author_sort | Guimiao Jiang |
| collection | DOAJ |
| description | Abstract Background With the development of large‐scale donkey farming in China, long‐distance transportation has become common practice, and the incidence of intestinal diseases after transportation has increased. The intestinal microbiota is important in health and disease, and whether or not transportation disturbs the intestinal microbiota in donkeys has not been investigated. Objectives To determine the effects of transportation on the fecal microbiota of healthy donkeys using 16S rRNA sequencing. Animals Fecal and blood samples were collected from 12 Dezhou donkeys before and after transportation. Methods Prospective controlled study. Cortisol, ACTH, and heat‐shock protein 90 (HSP90) concentrations were measured. Sequencing of 16S rRNA was used to assess the microbial composition. Alpha diversity and beta diversity were assessed. Results Results showed significant (P < .05) increases in cortisol (58.1 ± 14.6 to 71.1 ± 9.60 ng/mL), ACTH (163.8 ± 31.9 to 315.8 ± 27.9 pg/mL), and HSP90 (10.8 ± 1.67 to 14.6 ± 1.75 ng/mL) on the day of arrival. A significantly lower (P = .04) level of bacterial richness was found in fecal samples after transportation, compared with that before transportation without distinct changes in diversity. Most notably, donkeys had significant decreases in Atopostipes, Eubacterium, Streptococcus, and Coriobacteriaceae. Conclusions and Clinical Importance Transportation can induce stress in healthy donkeys and have some effect on the composition of the in fecal microbiota. Additional studies are required to understand the potential effect of these microbiota changes, especially significantly decreased bacteria, on the development intestinal diseases in donkeys during recovery from transportation. |
| first_indexed | 2024-12-22T13:56:07Z |
| format | Article |
| id | doaj.art-16950f9ff27849e096b3bf76567583d9 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-12-22T13:56:07Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-16950f9ff27849e096b3bf76567583d92022-12-21T18:23:32ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762021-09-013552449245710.1111/jvim.16235Transport stress affects the fecal microbiota in healthy donkeysGuimiao Jiang0Xinhao Zhang1Weiping Gao2Chuanliang Ji3Yantao Wang4Peixiang Feng5Yulong Feng6Zhiping Zhang7Lin Li8Fuwei Zhao9Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine Shenyang Agricultural University Shenyang ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaThe College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine Shenyang Agricultural University Shenyang ChinaNational Engineering Research Center for Gelatin‐based TCM, Dong‐E E‐Jiao Co., Ltd Liaocheng Shandong Province ChinaAbstract Background With the development of large‐scale donkey farming in China, long‐distance transportation has become common practice, and the incidence of intestinal diseases after transportation has increased. The intestinal microbiota is important in health and disease, and whether or not transportation disturbs the intestinal microbiota in donkeys has not been investigated. Objectives To determine the effects of transportation on the fecal microbiota of healthy donkeys using 16S rRNA sequencing. Animals Fecal and blood samples were collected from 12 Dezhou donkeys before and after transportation. Methods Prospective controlled study. Cortisol, ACTH, and heat‐shock protein 90 (HSP90) concentrations were measured. Sequencing of 16S rRNA was used to assess the microbial composition. Alpha diversity and beta diversity were assessed. Results Results showed significant (P < .05) increases in cortisol (58.1 ± 14.6 to 71.1 ± 9.60 ng/mL), ACTH (163.8 ± 31.9 to 315.8 ± 27.9 pg/mL), and HSP90 (10.8 ± 1.67 to 14.6 ± 1.75 ng/mL) on the day of arrival. A significantly lower (P = .04) level of bacterial richness was found in fecal samples after transportation, compared with that before transportation without distinct changes in diversity. Most notably, donkeys had significant decreases in Atopostipes, Eubacterium, Streptococcus, and Coriobacteriaceae. Conclusions and Clinical Importance Transportation can induce stress in healthy donkeys and have some effect on the composition of the in fecal microbiota. Additional studies are required to understand the potential effect of these microbiota changes, especially significantly decreased bacteria, on the development intestinal diseases in donkeys during recovery from transportation.https://doi.org/10.1111/jvim.16235donkeyfecal microbiotahormonal changestransport stress |
| spellingShingle | Guimiao Jiang Xinhao Zhang Weiping Gao Chuanliang Ji Yantao Wang Peixiang Feng Yulong Feng Zhiping Zhang Lin Li Fuwei Zhao Transport stress affects the fecal microbiota in healthy donkeys Journal of Veterinary Internal Medicine donkey fecal microbiota hormonal changes transport stress |
| title | Transport stress affects the fecal microbiota in healthy donkeys |
| title_full | Transport stress affects the fecal microbiota in healthy donkeys |
| title_fullStr | Transport stress affects the fecal microbiota in healthy donkeys |
| title_full_unstemmed | Transport stress affects the fecal microbiota in healthy donkeys |
| title_short | Transport stress affects the fecal microbiota in healthy donkeys |
| title_sort | transport stress affects the fecal microbiota in healthy donkeys |
| topic | donkey fecal microbiota hormonal changes transport stress |
| url | https://doi.org/10.1111/jvim.16235 |
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