Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study
Abstract The present study sought to evaluate the protective effect of Epigallocatechin-3-gallate (EGCG) and commercial green tea (GT) on eroded dentin using in vitro and in situ experimental models. For the in vitro experiment, matrix metalloproteinases (MMPs) were extracted from demineralized huma...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2021-11-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100281&tlng=en |
| _version_ | 1818945491308642304 |
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| author | Maria Denise Rodrigues DE MORAES Vanara Florêncio PASSOS Gislaine Cristina PADOVANI Lady Clarissa Brito da Rocha BEZERRA Ilka Maria VASCONCELOS Sérgio Lima SANTIAGO |
| author_facet | Maria Denise Rodrigues DE MORAES Vanara Florêncio PASSOS Gislaine Cristina PADOVANI Lady Clarissa Brito da Rocha BEZERRA Ilka Maria VASCONCELOS Sérgio Lima SANTIAGO |
| author_sort | Maria Denise Rodrigues DE MORAES |
| collection | DOAJ |
| description | Abstract The present study sought to evaluate the protective effect of Epigallocatechin-3-gallate (EGCG) and commercial green tea (GT) on eroded dentin using in vitro and in situ experimental models. For the in vitro experiment, matrix metalloproteinases (MMPs) were extracted from demineralized human coronary dentin powder (citric acid, pH 2.3) and assessed via a colorimetric assay and electrophoresis in gelatin. The gels were exposed to buffers with: control (no treatment), 0.05% sodium fluoride (NaF), 0.12% chlorhexidine digluconate (CHX), GT infusion, and 0.1% EGCG, and their respective activity was analyzed by zymography. For the in situ experiment, 20 healthy volunteers (aged 20-32 years) participated in this single-center, blind, crossover study. The subjects wore upper removable devices containing four human dentin blocks. Erosive challenge (coke-1 min) was performed four times/day/5 days. Blocks were treated for 1 min with: control (No treatment), 0.05% NaF, 0.1% EGCG, and GT. Thereafter, the specimens were subjected to stylus profilometry and SEM. ANOVA was used to evaluate dentin roughness and wear, with a significance level of 5%. In the zymography analysis, 0.12% CHX, GT, and 0.1% EGCG were found to inhibit the action of MMPs; however, in the colorimetric assay, only green tea inhibited the activity of MMPs. There were no significant differences observed in dentin roughness or wear (p > 0.05). Herein, EGCG and GT inhibited the activity of endogenous proteases, resulting in protection against erosion-induced dentin damage; however, they could not prevent tooth tissue loss in situ. |
| first_indexed | 2024-12-20T07:59:57Z |
| format | Article |
| id | doaj.art-1695c7c81a7f4c0289e780c187f8e567 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-20T07:59:57Z |
| publishDate | 2021-11-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-1695c7c81a7f4c0289e780c187f8e5672022-12-21T19:47:33ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072021-11-013510.1590/1807-3107bor-2021.vol35.0108Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ studyMaria Denise Rodrigues DE MORAEShttps://orcid.org/0000-0003-3977-2545Vanara Florêncio PASSOShttps://orcid.org/0000-0001-5121-0436Gislaine Cristina PADOVANIhttps://orcid.org/0000-0003-4053-2530Lady Clarissa Brito da Rocha BEZERRAhttps://orcid.org/0000-0002-5910-9129Ilka Maria VASCONCELOShttps://orcid.org/0000-0002-7207-8734Sérgio Lima SANTIAGOhttps://orcid.org/0000-0002-6808-092XAbstract The present study sought to evaluate the protective effect of Epigallocatechin-3-gallate (EGCG) and commercial green tea (GT) on eroded dentin using in vitro and in situ experimental models. For the in vitro experiment, matrix metalloproteinases (MMPs) were extracted from demineralized human coronary dentin powder (citric acid, pH 2.3) and assessed via a colorimetric assay and electrophoresis in gelatin. The gels were exposed to buffers with: control (no treatment), 0.05% sodium fluoride (NaF), 0.12% chlorhexidine digluconate (CHX), GT infusion, and 0.1% EGCG, and their respective activity was analyzed by zymography. For the in situ experiment, 20 healthy volunteers (aged 20-32 years) participated in this single-center, blind, crossover study. The subjects wore upper removable devices containing four human dentin blocks. Erosive challenge (coke-1 min) was performed four times/day/5 days. Blocks were treated for 1 min with: control (No treatment), 0.05% NaF, 0.1% EGCG, and GT. Thereafter, the specimens were subjected to stylus profilometry and SEM. ANOVA was used to evaluate dentin roughness and wear, with a significance level of 5%. In the zymography analysis, 0.12% CHX, GT, and 0.1% EGCG were found to inhibit the action of MMPs; however, in the colorimetric assay, only green tea inhibited the activity of MMPs. There were no significant differences observed in dentin roughness or wear (p > 0.05). Herein, EGCG and GT inhibited the activity of endogenous proteases, resulting in protection against erosion-induced dentin damage; however, they could not prevent tooth tissue loss in situ.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100281&tlng=enTooth ErosionTooth WearCollagenMatrix MetalloproteinasesCamellia sinensis |
| spellingShingle | Maria Denise Rodrigues DE MORAES Vanara Florêncio PASSOS Gislaine Cristina PADOVANI Lady Clarissa Brito da Rocha BEZERRA Ilka Maria VASCONCELOS Sérgio Lima SANTIAGO Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study Brazilian Oral Research Tooth Erosion Tooth Wear Collagen Matrix Metalloproteinases Camellia sinensis |
| title | Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study |
| title_full | Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study |
| title_fullStr | Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study |
| title_full_unstemmed | Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study |
| title_short | Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study |
| title_sort | protective effect of green tea catechins on eroded human dentin an in vitro in situ study |
| topic | Tooth Erosion Tooth Wear Collagen Matrix Metalloproteinases Camellia sinensis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100281&tlng=en |
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