In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells

In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells

Summary: Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro...

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Bibliographic Details
Main Authors: Renée R.C.E. Schreurs, Martin E. Baumdick, Agata Drewniak, Madeleine J. Bunders
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell culture
Immunology
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002264

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