Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers
Summary: Metabolism is important for the regulation of hematopoietic stem cells (HSCs) and drives cellular fate. Due to the scarcity of HSCs, it has been technically challenging to perform metabolome analyses gaining insight into HSC metabolic regulatory networks. Here, we present two targeted liqui...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200288X |
| _version_ | 1817980421764284416 |
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| author | Katharina Schönberger Michael Mitterer Joerg M. Buescher Nina Cabezas-Wallscheid |
| author_facet | Katharina Schönberger Michael Mitterer Joerg M. Buescher Nina Cabezas-Wallscheid |
| author_sort | Katharina Schönberger |
| collection | DOAJ |
| description | Summary: Metabolism is important for the regulation of hematopoietic stem cells (HSCs) and drives cellular fate. Due to the scarcity of HSCs, it has been technically challenging to perform metabolome analyses gaining insight into HSC metabolic regulatory networks. Here, we present two targeted liquid chromatography–mass spectrometry approaches that enable the detection of metabolites after fluorescence-activated cell sorting when sample amounts are limited. One protocol covers signaling lipids and retinoids, while the second detects tricarboxylic acid cycle metabolites and amino acids.For complete details on the use and execution of this protocol, please refer to Schönberger et al. (2022). : Publisher's note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T22:53:42Z |
| format | Article |
| id | doaj.art-169b196d841a48ba970b95799f328d22 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T22:53:42Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-169b196d841a48ba970b95799f328d222022-12-22T02:26:06ZengElsevierSTAR Protocols2666-16672022-06-0132101408Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbersKatharina Schönberger0Michael Mitterer1Joerg M. Buescher2Nina Cabezas-Wallscheid3Max Planck Institute of Immunobiology and Epigenetics, Department of Cellular and Molecular Immunology, Stübeweg 51, 79108 Freiburg, Germany; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany; International Max Planck Research School for Molecular and Cellular Biology (IMPRS-MCB), Freiburg, GermanyMax Planck Institute of Immunobiology and Epigenetics, Metabolomics Core Facility, Stübeweg 51, 79108 Freiburg, GermanyMax Planck Institute of Immunobiology and Epigenetics, Metabolomics Core Facility, Stübeweg 51, 79108 Freiburg, GermanyMax Planck Institute of Immunobiology and Epigenetics, Department of Cellular and Molecular Immunology, Stübeweg 51, 79108 Freiburg, Germany; Corresponding authorSummary: Metabolism is important for the regulation of hematopoietic stem cells (HSCs) and drives cellular fate. Due to the scarcity of HSCs, it has been technically challenging to perform metabolome analyses gaining insight into HSC metabolic regulatory networks. Here, we present two targeted liquid chromatography–mass spectrometry approaches that enable the detection of metabolites after fluorescence-activated cell sorting when sample amounts are limited. One protocol covers signaling lipids and retinoids, while the second detects tricarboxylic acid cycle metabolites and amino acids.For complete details on the use and execution of this protocol, please refer to Schönberger et al. (2022). : Publisher's note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672200288XMetabolomicsStem CellsMass Spectrometry |
| spellingShingle | Katharina Schönberger Michael Mitterer Joerg M. Buescher Nina Cabezas-Wallscheid Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers STAR Protocols Metabolomics Stem Cells Mass Spectrometry |
| title | Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers |
| title_full | Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers |
| title_fullStr | Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers |
| title_full_unstemmed | Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers |
| title_short | Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers |
| title_sort | targeted lc ms ms based metabolomics and lipidomics on limited hematopoietic stem cell numbers |
| topic | Metabolomics Stem Cells Mass Spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200288X |
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