Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study
Increasing antimicrobial resistance (AMR) colonizing domesticated animals is a global concern threatening food safety. This study aimed at determining the prevalence of multidrug resistance (MDR) and epidemiological cut-off values (ECVs) of <i>E. coli</i> isolated from poultry and pigs i...
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MDPI AG
2022-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/7/835 |
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| author | Conjester I. Mtemisika Helmut Nyawale Ronald J. Benju Joseph M. Genchwere Vitus Silago Martha F. Mushi Joseph Mwanga Eveline Konje Mariam M. Mirambo Stephen E. Mshana |
| author_facet | Conjester I. Mtemisika Helmut Nyawale Ronald J. Benju Joseph M. Genchwere Vitus Silago Martha F. Mushi Joseph Mwanga Eveline Konje Mariam M. Mirambo Stephen E. Mshana |
| author_sort | Conjester I. Mtemisika |
| collection | DOAJ |
| description | Increasing antimicrobial resistance (AMR) colonizing domesticated animals is a global concern threatening food safety. This study aimed at determining the prevalence of multidrug resistance (MDR) and epidemiological cut-off values (ECVs) of <i>E. coli</i> isolated from poultry and pigs in Mwanza, Tanzania. This cross-sectional study was conducted between June and August 2021, involving 297 pigs, 191 broilers, and 203 layers. Rectal and cloacal swabs were collected and processed following standard guidelines. ECVs were determined using normalized resistance interpretation (NRI), a computer software, and descriptive analysis was performed using STATA version 13.0. The overall prevalence of MDR <i>E.</i><i>coli</i> was 63.2%, whereas poultry (87.5% layers and 86.3% broilers) were more colonized than pigs (31.8%) (<i>p</i> < 0.001). Based on ECVs of antibiotics tested, <i>E. coli</i> from broilers, layers, and pigs exhibited different resistance patterns hence different populations. Exotic breed (<i>p</i> < 0.001) and recent antimicrobial use (<i>p</i> < 0.001) significantly predicted colonization with MDR <i>E. coli</i>. Veterinary officers should implement regulations that prohibit the inappropriate use of antimicrobial agents in livestock keeping. |
| first_indexed | 2024-03-09T12:11:27Z |
| format | Article |
| id | doaj.art-169c33127cfa42ada64087e9e07e8983 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T12:11:27Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-169c33127cfa42ada64087e9e07e89832023-11-30T22:51:03ZengMDPI AGAnimals2076-26152022-03-0112783510.3390/ani12070835Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section StudyConjester I. Mtemisika0Helmut Nyawale1Ronald J. Benju2Joseph M. Genchwere3Vitus Silago4Martha F. Mushi5Joseph Mwanga6Eveline Konje7Mariam M. Mirambo8Stephen E. Mshana9Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaTanzania Veterinary Laboratory Agency, Ministry of Livestock and Fisheries, Mwanza P.O. Box 129, TanzaniaTanzania Veterinary Laboratory Agency, Ministry of Livestock and Fisheries, Mwanza P.O. Box 129, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Epidemiology, Biostatistics and Behavioral Sciences, School of Public Health, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Epidemiology, Biostatistics and Behavioral Sciences, School of Public Health, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaIncreasing antimicrobial resistance (AMR) colonizing domesticated animals is a global concern threatening food safety. This study aimed at determining the prevalence of multidrug resistance (MDR) and epidemiological cut-off values (ECVs) of <i>E. coli</i> isolated from poultry and pigs in Mwanza, Tanzania. This cross-sectional study was conducted between June and August 2021, involving 297 pigs, 191 broilers, and 203 layers. Rectal and cloacal swabs were collected and processed following standard guidelines. ECVs were determined using normalized resistance interpretation (NRI), a computer software, and descriptive analysis was performed using STATA version 13.0. The overall prevalence of MDR <i>E.</i><i>coli</i> was 63.2%, whereas poultry (87.5% layers and 86.3% broilers) were more colonized than pigs (31.8%) (<i>p</i> < 0.001). Based on ECVs of antibiotics tested, <i>E. coli</i> from broilers, layers, and pigs exhibited different resistance patterns hence different populations. Exotic breed (<i>p</i> < 0.001) and recent antimicrobial use (<i>p</i> < 0.001) significantly predicted colonization with MDR <i>E. coli</i>. Veterinary officers should implement regulations that prohibit the inappropriate use of antimicrobial agents in livestock keeping.https://www.mdpi.com/2076-2615/12/7/835antimicrobial resistanceepidemiological cut-off values<i>Escherichia coli</i>poultrypigs |
| spellingShingle | Conjester I. Mtemisika Helmut Nyawale Ronald J. Benju Joseph M. Genchwere Vitus Silago Martha F. Mushi Joseph Mwanga Eveline Konje Mariam M. Mirambo Stephen E. Mshana Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study Animals antimicrobial resistance epidemiological cut-off values <i>Escherichia coli</i> poultry pigs |
| title | Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study |
| title_full | Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study |
| title_fullStr | Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study |
| title_full_unstemmed | Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study |
| title_short | Epidemiological Cut-Off Values and Multidrug Resistance of <i>Escherichia coli</i> Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study |
| title_sort | epidemiological cut off values and multidrug resistance of i escherichia coli i isolated from domesticated poultry and pigs reared in mwanza tanzania a cross section study |
| topic | antimicrobial resistance epidemiological cut-off values <i>Escherichia coli</i> poultry pigs |
| url | https://www.mdpi.com/2076-2615/12/7/835 |
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