Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea
This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2023-09-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/upload/pdf/RPD-2023-29-3-276.pdf |
| _version_ | 1827793530383237120 |
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| author | Hong-Kyu Lee Seongju Han Sangmin Bak Minseok Kim Jean Geung Min Hak ju Kim Dong Hyun Kang Minhui Kim Wonyoung Jeong Seungbin Baek Minjoo Yang Taegun Lim Chanhoon An Tae-Dong Kim Chung Youl Park Jae Sun Moon Su-Heon Lee |
| author_facet | Hong-Kyu Lee Seongju Han Sangmin Bak Minseok Kim Jean Geung Min Hak ju Kim Dong Hyun Kang Minhui Kim Wonyoung Jeong Seungbin Baek Minjoo Yang Taegun Lim Chanhoon An Tae-Dong Kim Chung Youl Park Jae Sun Moon Su-Heon Lee |
| author_sort | Hong-Kyu Lee |
| collection | DOAJ |
| description | This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associ-ated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea. |
| first_indexed | 2024-03-11T18:19:48Z |
| format | Article |
| id | doaj.art-169c68f633c8419d9f892317212b426b |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-03-11T18:19:48Z |
| publishDate | 2023-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-169c68f633c8419d9f892317212b426b2023-10-15T23:30:41ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912023-09-0129327628510.5423/RPD.2023.29.3.2761808Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in KoreaHong-Kyu Lee0Seongju Han1Sangmin Bak2Minseok Kim3Jean Geung Min4Hak ju Kim5Dong Hyun Kang6Minhui Kim7Wonyoung Jeong8Seungbin Baek9Minjoo Yang10Taegun Lim11Chanhoon An12Tae-Dong Kim13Chung Youl Park14Jae Sun Moon15Su-Heon Lee16School of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biology, Kyungpook National University, Daegu 41566, KoreaDivision of Forest Tree Improvement and Biotechnology, National Institute of Forest Science, Suwon 16631, KoreaDivision of Special Forest Resources, National Institute of Forest Science, Suwon 16631, KoreaDivision of Wild Plant Seed Research, Baekdudaegan National Arboretum, Bonghwa 36209, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu 41566, KoreaThis work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associ-ated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.http://www.online-rpd.org/upload/pdf/RPD-2023-29-3-276.pdfjubvjujubejumavjymavmetatranscriptome analysis |
| spellingShingle | Hong-Kyu Lee Seongju Han Sangmin Bak Minseok Kim Jean Geung Min Hak ju Kim Dong Hyun Kang Minhui Kim Wonyoung Jeong Seungbin Baek Minjoo Yang Taegun Lim Chanhoon An Tae-Dong Kim Chung Youl Park Jae Sun Moon Su-Heon Lee Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea Research in Plant Disease jubv jujube jumav jymav metatranscriptome analysis |
| title | Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea |
| title_full | Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea |
| title_fullStr | Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea |
| title_full_unstemmed | Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea |
| title_short | Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea |
| title_sort | metatranscriptome based analysis of viral incidence in jujube ziziphus jujuba in korea |
| topic | jubv jujube jumav jymav metatranscriptome analysis |
| url | http://www.online-rpd.org/upload/pdf/RPD-2023-29-3-276.pdf |
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