Isolation and identification of bacteria from rotary drum compost of water hyacinth
Abstract Purpose The present work aims to isolate and identify bacterial community from the rotary drum compost of green waste such as the water hyacinth. Compost quality was also investigated with the physicochemical parameters and the heavy metal analysis. Methods For rotary drum composting, a was...
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| Format: | Article |
| Language: | English |
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Islamic Azad University-Isfahan (Khorasgan) Branch- Iran
2017-08-01
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| Series: | International Journal of Recycling of Organic Waste in Agriculture |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007/s40093-017-0172-8 |
| _version_ | 1797332162980085760 |
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| author | Isha Vishan Senthilkumar Sivaprakasam Ajay Kalamdhad |
| author_facet | Isha Vishan Senthilkumar Sivaprakasam Ajay Kalamdhad |
| author_sort | Isha Vishan |
| collection | DOAJ |
| description | Abstract Purpose The present work aims to isolate and identify bacterial community from the rotary drum compost of green waste such as the water hyacinth. Compost quality was also investigated with the physicochemical parameters and the heavy metal analysis. Methods For rotary drum composting, a waste mixture of 150 kg with water hyacinth, cow dung and sawdust were prepared in the proportion of 6:3:1, respectively. The physicochemical parameters such as pH, temperature, volatile solids and the electrical conductivity were analyzed to detect the compost quality. Pb, Ni, Zn, and Cd were investigated for total heavy metals, toxicity characteristic leaching procedure (TCLP), diethylenetriaminepentaacetic acid (DTPA) and water solubility tests. Consistent and active bacterial community were isolated from the rotary drum compost of water hyacinth. Culture-dependent and culture-independent techniques were approached for the isolation process. Results Twelve bacteria were isolated and identified by 16S rDNA sequencing and phylogenetic analysis; they majorly belonged to the Bacillus and Enterobacter family. The analysis of temperature, pH, EC, VS and heavy metals depicted the good quality of compost. Heavy metals concentration was in Pb > Ni > Zn > Cd; however, for Pb, Cd and Ni water solubility remained non-detectable, DTPA concentration for Pb and Cd was also found to be non-detectable. Conclusions The microbes in water hyacinth compost are metabolically active degrading the organic matter, surviving in the heavy metal-loaded compost environment. Thus, green waste (water hyacinth) can be utilized to isolate bacterial species in anticipation of their application in heavy metal removal in liquid and solid waste sources for micro-bioremediation. |
| first_indexed | 2024-03-08T07:45:35Z |
| format | Article |
| id | doaj.art-169da5073594408bade5d172686e3ef6 |
| institution | Directory Open Access Journal |
| issn | 2195-3228 2251-7715 |
| language | English |
| last_indexed | 2024-03-08T07:45:35Z |
| publishDate | 2017-08-01 |
| publisher | Islamic Azad University-Isfahan (Khorasgan) Branch- Iran |
| record_format | Article |
| series | International Journal of Recycling of Organic Waste in Agriculture |
| spelling | doaj.art-169da5073594408bade5d172686e3ef62024-02-02T16:16:17ZengIslamic Azad University-Isfahan (Khorasgan) Branch- IranInternational Journal of Recycling of Organic Waste in Agriculture2195-32282251-77152017-08-016324525310.1007/s40093-017-0172-8Isolation and identification of bacteria from rotary drum compost of water hyacinthIsha Vishan0Senthilkumar Sivaprakasam1Ajay Kalamdhad2Centre for the Environment, Indian Institute of Technology Guwahati (IIT G)Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati (IIT G)Department of Civil Engineering, Indian Institute of Technology Guwahati (IITG)Abstract Purpose The present work aims to isolate and identify bacterial community from the rotary drum compost of green waste such as the water hyacinth. Compost quality was also investigated with the physicochemical parameters and the heavy metal analysis. Methods For rotary drum composting, a waste mixture of 150 kg with water hyacinth, cow dung and sawdust were prepared in the proportion of 6:3:1, respectively. The physicochemical parameters such as pH, temperature, volatile solids and the electrical conductivity were analyzed to detect the compost quality. Pb, Ni, Zn, and Cd were investigated for total heavy metals, toxicity characteristic leaching procedure (TCLP), diethylenetriaminepentaacetic acid (DTPA) and water solubility tests. Consistent and active bacterial community were isolated from the rotary drum compost of water hyacinth. Culture-dependent and culture-independent techniques were approached for the isolation process. Results Twelve bacteria were isolated and identified by 16S rDNA sequencing and phylogenetic analysis; they majorly belonged to the Bacillus and Enterobacter family. The analysis of temperature, pH, EC, VS and heavy metals depicted the good quality of compost. Heavy metals concentration was in Pb > Ni > Zn > Cd; however, for Pb, Cd and Ni water solubility remained non-detectable, DTPA concentration for Pb and Cd was also found to be non-detectable. Conclusions The microbes in water hyacinth compost are metabolically active degrading the organic matter, surviving in the heavy metal-loaded compost environment. Thus, green waste (water hyacinth) can be utilized to isolate bacterial species in anticipation of their application in heavy metal removal in liquid and solid waste sources for micro-bioremediation.http://link.springer.com/article/10.1007/s40093-017-0172-8Rotary drum compostingBacillusEnterobacterHeavy metalsPCR (polymerase chain reaction)Micro-bioremediation |
| spellingShingle | Isha Vishan Senthilkumar Sivaprakasam Ajay Kalamdhad Isolation and identification of bacteria from rotary drum compost of water hyacinth International Journal of Recycling of Organic Waste in Agriculture Rotary drum composting Bacillus Enterobacter Heavy metals PCR (polymerase chain reaction) Micro-bioremediation |
| title | Isolation and identification of bacteria from rotary drum compost of water hyacinth |
| title_full | Isolation and identification of bacteria from rotary drum compost of water hyacinth |
| title_fullStr | Isolation and identification of bacteria from rotary drum compost of water hyacinth |
| title_full_unstemmed | Isolation and identification of bacteria from rotary drum compost of water hyacinth |
| title_short | Isolation and identification of bacteria from rotary drum compost of water hyacinth |
| title_sort | isolation and identification of bacteria from rotary drum compost of water hyacinth |
| topic | Rotary drum composting Bacillus Enterobacter Heavy metals PCR (polymerase chain reaction) Micro-bioremediation |
| url | http://link.springer.com/article/10.1007/s40093-017-0172-8 |
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