Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study
This study aims at evaluating the performances of the multiplex PCR Allplex<sup>TM</sup> Gastrointestinal Panel-Parasite Assay (GIPPA), which detects <i>G. duodenalis</i>, <i>Cryptosporidium</i> spp., <i>E. histolytica</i>, <i>D. fragilis</i&g...
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MDPI AG
2020-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/4/569 |
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| author | Brice Autier Jean-Pierre Gangneux Florence Robert-Gangneux |
| author_facet | Brice Autier Jean-Pierre Gangneux Florence Robert-Gangneux |
| author_sort | Brice Autier |
| collection | DOAJ |
| description | This study aims at evaluating the performances of the multiplex PCR Allplex<sup>TM</sup> Gastrointestinal Panel-Parasite Assay (GIPPA), which detects <i>G. duodenalis</i>, <i>Cryptosporidium</i> spp., <i>E. histolytica</i>, <i>D. fragilis</i>, <i>B. hominis</i>, and <i>C. cayetanensis</i>, by comparison to microscopy. A retrospective evaluation was conducted on a series of positive clinical samples (<i>n</i> = 99) stored at −80 °C or at +4 °C. A five-month prospective study was then conducted on all samples sent to our lab for parasite detection (<i>n</i> = 586). In the retrospective cohort, sensitivity was 81% for both <i>G. duodenalis</i> (26/32) and <i>D. fragilis</i> (21/26) and 100% for <i>Cryptosporidium</i> spp. (26/26, including 6 different species), <i>B. hominis</i> (26/26), and <i>C. cayetanensis</i> (4/4). During the prospective study, 95 samples were positive by microscopy and 207 by multiplex PCR assay. The molecular assay showed a significantly higher sensitivity of PCR, especially for <i>G. duodenalis</i> (100% vs. 60.7%, <i>p</i> < 0.01), <i>D. fragilis</i> (97.2% vs. 14.1%, <i>p</i> < 0.001), and <i>B. hominis</i> (99.4% vs. 44.2%, <i>p</i> < 0.001) but also for <i>E. histolytica</i> (100% vs. 50.0%). The sensitivity of the Allplex<sup>TM</sup> GIPPA on the first stool sample was equivalent to the sensitivity of microscopy on multiple stool samples but inferior to multiplex PCR on multiple stool samples. Taken together, the Allplex<sup>TM</sup> GIPPA is suitable for the routine detection of protozoa in fecal samples. |
| first_indexed | 2024-03-10T20:27:45Z |
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| id | doaj.art-169e326f92bc4a76978534a49726eaa2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:27:45Z |
| publishDate | 2020-04-01 |
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| series | Microorganisms |
| spelling | doaj.art-169e326f92bc4a76978534a49726eaa22023-11-19T21:39:14ZengMDPI AGMicroorganisms2076-26072020-04-018456910.3390/microorganisms8040569Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective StudyBrice Autier0Jean-Pierre Gangneux1Florence Robert-Gangneux2Irset (Institut de Recherche en Santé Environnement Travail), Univ Rennes, CHU Rennes, Inserm, EHESP, UMR_S 1085, 35000 Rennes, FranceIrset (Institut de Recherche en Santé Environnement Travail), Univ Rennes, CHU Rennes, Inserm, EHESP, UMR_S 1085, 35000 Rennes, FranceIrset (Institut de Recherche en Santé Environnement Travail), Univ Rennes, CHU Rennes, Inserm, EHESP, UMR_S 1085, 35000 Rennes, FranceThis study aims at evaluating the performances of the multiplex PCR Allplex<sup>TM</sup> Gastrointestinal Panel-Parasite Assay (GIPPA), which detects <i>G. duodenalis</i>, <i>Cryptosporidium</i> spp., <i>E. histolytica</i>, <i>D. fragilis</i>, <i>B. hominis</i>, and <i>C. cayetanensis</i>, by comparison to microscopy. A retrospective evaluation was conducted on a series of positive clinical samples (<i>n</i> = 99) stored at −80 °C or at +4 °C. A five-month prospective study was then conducted on all samples sent to our lab for parasite detection (<i>n</i> = 586). In the retrospective cohort, sensitivity was 81% for both <i>G. duodenalis</i> (26/32) and <i>D. fragilis</i> (21/26) and 100% for <i>Cryptosporidium</i> spp. (26/26, including 6 different species), <i>B. hominis</i> (26/26), and <i>C. cayetanensis</i> (4/4). During the prospective study, 95 samples were positive by microscopy and 207 by multiplex PCR assay. The molecular assay showed a significantly higher sensitivity of PCR, especially for <i>G. duodenalis</i> (100% vs. 60.7%, <i>p</i> < 0.01), <i>D. fragilis</i> (97.2% vs. 14.1%, <i>p</i> < 0.001), and <i>B. hominis</i> (99.4% vs. 44.2%, <i>p</i> < 0.001) but also for <i>E. histolytica</i> (100% vs. 50.0%). The sensitivity of the Allplex<sup>TM</sup> GIPPA on the first stool sample was equivalent to the sensitivity of microscopy on multiple stool samples but inferior to multiplex PCR on multiple stool samples. Taken together, the Allplex<sup>TM</sup> GIPPA is suitable for the routine detection of protozoa in fecal samples.https://www.mdpi.com/2076-2607/8/4/569<i>Giardia duodenalis</i><i>Entamoeba histolytica</i><i>Cryptosporidium</i> spp.<i>Dientamoeba fragilis</i><i>Cyclospora cayetanensis</i><i>Blastocystis hominis</i> |
| spellingShingle | Brice Autier Jean-Pierre Gangneux Florence Robert-Gangneux Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study Microorganisms <i>Giardia duodenalis</i> <i>Entamoeba histolytica</i> <i>Cryptosporidium</i> spp. <i>Dientamoeba fragilis</i> <i>Cyclospora cayetanensis</i> <i>Blastocystis hominis</i> |
| title | Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study |
| title_full | Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study |
| title_fullStr | Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study |
| title_full_unstemmed | Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study |
| title_short | Evaluation of the Allplex<sup>TM</sup> Gastrointestinal Panel—Parasite Assay for Protozoa Detection in Stool Samples: A Retrospective and Prospective Study |
| title_sort | evaluation of the allplex sup tm sup gastrointestinal panel parasite assay for protozoa detection in stool samples a retrospective and prospective study |
| topic | <i>Giardia duodenalis</i> <i>Entamoeba histolytica</i> <i>Cryptosporidium</i> spp. <i>Dientamoeba fragilis</i> <i>Cyclospora cayetanensis</i> <i>Blastocystis hominis</i> |
| url | https://www.mdpi.com/2076-2607/8/4/569 |
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