Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate
The concentration of glucose in the body's fluids is an important parameter that can indicate pathological conditions such as the progress of infected wounds. Several wearables and implantable detection approaches have been developed with high selectivity and sensitivity for glucose. However, a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422000564 |
| _version_ | 1818547928250187776 |
|---|---|
| author | Giorgia Giovannini Paolo Cinelli Luciano F. Boesel René M. Rossi |
| author_facet | Giorgia Giovannini Paolo Cinelli Luciano F. Boesel René M. Rossi |
| author_sort | Giorgia Giovannini |
| collection | DOAJ |
| description | The concentration of glucose in the body's fluids is an important parameter that can indicate pathological conditions such as the progress of infected wounds. Several wearables and implantable detection approaches have been developed with high selectivity and sensitivity for glucose. However, all of them have drawbacks such as low stability, limited selectivity, and often require complex technology. In this work, we present a fluorescent-based cost-efficient imprinted hydrogel (MIH_GSH) capable of detecting glucose within 30 min. The imprinting approach allows us to improve the selectivity for glucose, overcoming the low specificity and limited binding efficiency at neutral pH of boronic acid-based detection mechanisms. The binding affinity determined for glucose-MIH_GSH was indeed 6-fold higher than the one determined for the non-imprinted hydrogel with a calculated imprinting factor of 1.7. The limit of detection of MIH_GSH for glucose in artificial wound exudate was calculated as 0.48 mM at pH 7.4 proving the suitability of the proposed approach to diagnose chronic wounds (ca. 1 mM). MIH_GSH was compared with a commercial colorimetric assay for the quantification of glucose in wound exudate specimens collected from hospitalized patients. The results obtained with the two methods were statistically similar confirming the robustness of our approach. Importantly, whereas with the colorimetric assay sample preparation was required to limit the interference of the sample background, the fluorescent signal of MIH_GSH was not affected even when used to measure glucose directly in bloody samples. The sensing mechanism here proposed can pave the way for the development of cost-efficient and wearable point-of-care tools capable of monitoring the glucose level in wound exudate enabling the quick assessment of chronic injuries. |
| first_indexed | 2024-12-12T08:13:12Z |
| format | Article |
| id | doaj.art-169e4d6bd8bd43f092bdb8aa27a14996 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-12-12T08:13:12Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-169e4d6bd8bd43f092bdb8aa27a149962022-12-22T00:31:42ZengElsevierMaterials Today Bio2590-00642022-03-0114100258Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudateGiorgia Giovannini0Paolo Cinelli1Luciano F. Boesel2René M. Rossi3Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014, St.Gallen, Switzerland; Corresponding author.Department of Trauma, University of Zurich, Zurich, SwitzerlandEmpa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014, St.Gallen, SwitzerlandEmpa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014, St.Gallen, Switzerland; Corresponding author.The concentration of glucose in the body's fluids is an important parameter that can indicate pathological conditions such as the progress of infected wounds. Several wearables and implantable detection approaches have been developed with high selectivity and sensitivity for glucose. However, all of them have drawbacks such as low stability, limited selectivity, and often require complex technology. In this work, we present a fluorescent-based cost-efficient imprinted hydrogel (MIH_GSH) capable of detecting glucose within 30 min. The imprinting approach allows us to improve the selectivity for glucose, overcoming the low specificity and limited binding efficiency at neutral pH of boronic acid-based detection mechanisms. The binding affinity determined for glucose-MIH_GSH was indeed 6-fold higher than the one determined for the non-imprinted hydrogel with a calculated imprinting factor of 1.7. The limit of detection of MIH_GSH for glucose in artificial wound exudate was calculated as 0.48 mM at pH 7.4 proving the suitability of the proposed approach to diagnose chronic wounds (ca. 1 mM). MIH_GSH was compared with a commercial colorimetric assay for the quantification of glucose in wound exudate specimens collected from hospitalized patients. The results obtained with the two methods were statistically similar confirming the robustness of our approach. Importantly, whereas with the colorimetric assay sample preparation was required to limit the interference of the sample background, the fluorescent signal of MIH_GSH was not affected even when used to measure glucose directly in bloody samples. The sensing mechanism here proposed can pave the way for the development of cost-efficient and wearable point-of-care tools capable of monitoring the glucose level in wound exudate enabling the quick assessment of chronic injuries.http://www.sciencedirect.com/science/article/pii/S2590006422000564FluorescenceGlucose detectionMolecularly imprinted hydrogelThioflavinBoronic acidWound exudate |
| spellingShingle | Giorgia Giovannini Paolo Cinelli Luciano F. Boesel René M. Rossi Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate Materials Today Bio Fluorescence Glucose detection Molecularly imprinted hydrogel Thioflavin Boronic acid Wound exudate |
| title | Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate |
| title_full | Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate |
| title_fullStr | Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate |
| title_full_unstemmed | Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate |
| title_short | Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate |
| title_sort | thioflavin modified molecularly imprinted hydrogel for fluorescent based non enzymatic glucose detection in wound exudate |
| topic | Fluorescence Glucose detection Molecularly imprinted hydrogel Thioflavin Boronic acid Wound exudate |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422000564 |
| work_keys_str_mv | AT giorgiagiovannini thioflavinmodifiedmolecularlyimprintedhydrogelforfluorescentbasednonenzymaticglucosedetectioninwoundexudate AT paolocinelli thioflavinmodifiedmolecularlyimprintedhydrogelforfluorescentbasednonenzymaticglucosedetectioninwoundexudate AT lucianofboesel thioflavinmodifiedmolecularlyimprintedhydrogelforfluorescentbasednonenzymaticglucosedetectioninwoundexudate AT renemrossi thioflavinmodifiedmolecularlyimprintedhydrogelforfluorescentbasednonenzymaticglucosedetectioninwoundexudate |