In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish
ABSTRACT This study aimed to isolate and select in vitro bacteria with probiotic potential for the Amazon ornamental fish Nannostomus beckfordi. For isolate, twelve fish underwent surgery procedure to remove their intestinal tract, macerate and then inoculate in the plate petri containing de Man Rug...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2022-04-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100111&tlng=en |
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| author | E.M. Lopes A.V. Silva F.A.L. Barros A.F.L. Santos C.A.M. Cordeiro M.V.S. Couto P.E.G. Paixão R.Y. Fujimoto N.C. Sousa |
| author_facet | E.M. Lopes A.V. Silva F.A.L. Barros A.F.L. Santos C.A.M. Cordeiro M.V.S. Couto P.E.G. Paixão R.Y. Fujimoto N.C. Sousa |
| author_sort | E.M. Lopes |
| collection | DOAJ |
| description | ABSTRACT This study aimed to isolate and select in vitro bacteria with probiotic potential for the Amazon ornamental fish Nannostomus beckfordi. For isolate, twelve fish underwent surgery procedure to remove their intestinal tract, macerate and then inoculate in the plate petri containing de Man Rugosa Sharped Agar (MRS). After bacterial growth (48 hours at 35ºC), selected strains were inoculated in MRS broth and submitted to resistance test with NaCl (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%), pH (4, 5, 6, 8 and 9) and bile salts (5% w/v). Inhibition test against pathogenic bacteria Aeromonas hydrophila, Pseudomonas aeroginosa, Streptococcus agalactiae and Aeromonas Jandaei was also performed. Within the isolated strains group (23 strains), only six (S1, S2, S3, S4, S5 and S6) showed probiotic potential. Strains S1 and S6 showed the greater resistance for NaCl (0.5% and 1%) and pH (5 and 6), but only S1 obtained better results to resist the bile salts. Even against pathogenic bacteria, the S1 showed the best results with inhibition halos greater than 9 mm. In the end, this bacterial strain (S1) was identified as Enterococcus faecium 11037CHB. Thus, this is the first report regarding isolated autochthonous bacterium E. faecium with probiotic potential of N. beckfordi. |
| first_indexed | 2024-12-11T07:05:14Z |
| format | Article |
| id | doaj.art-169e8b430eec424f82f4f75395ff7cbb |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-11T07:05:14Z |
| publishDate | 2022-04-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-169e8b430eec424f82f4f75395ff7cbb2022-12-22T01:16:31ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622022-04-0174111111610.1590/1678-4162-12179In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fishE.M. Lopeshttps://orcid.org/0000-0002-1165-5665A.V. Silvahttps://orcid.org/0000-0002-7801-6761F.A.L. Barroshttps://orcid.org/0000-0002-2392-6266A.F.L. Santoshttps://orcid.org/0000-0002-9743-5814C.A.M. Cordeirohttps://orcid.org/0000-0002-2698-6918M.V.S. Coutohttps://orcid.org/0000-0001-9235-0996P.E.G. Paixãohttps://orcid.org/0000-0002-8949-4232R.Y. Fujimotohttps://orcid.org/0000-0002-5039-4445N.C. Sousahttps://orcid.org/0000-0002-7580-5650ABSTRACT This study aimed to isolate and select in vitro bacteria with probiotic potential for the Amazon ornamental fish Nannostomus beckfordi. For isolate, twelve fish underwent surgery procedure to remove their intestinal tract, macerate and then inoculate in the plate petri containing de Man Rugosa Sharped Agar (MRS). After bacterial growth (48 hours at 35ºC), selected strains were inoculated in MRS broth and submitted to resistance test with NaCl (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%), pH (4, 5, 6, 8 and 9) and bile salts (5% w/v). Inhibition test against pathogenic bacteria Aeromonas hydrophila, Pseudomonas aeroginosa, Streptococcus agalactiae and Aeromonas Jandaei was also performed. Within the isolated strains group (23 strains), only six (S1, S2, S3, S4, S5 and S6) showed probiotic potential. Strains S1 and S6 showed the greater resistance for NaCl (0.5% and 1%) and pH (5 and 6), but only S1 obtained better results to resist the bile salts. Even against pathogenic bacteria, the S1 showed the best results with inhibition halos greater than 9 mm. In the end, this bacterial strain (S1) was identified as Enterococcus faecium 11037CHB. Thus, this is the first report regarding isolated autochthonous bacterium E. faecium with probiotic potential of N. beckfordi.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100111&tlng=enAmazon ornamental fishautochthonous probioticin vitro assay |
| spellingShingle | E.M. Lopes A.V. Silva F.A.L. Barros A.F.L. Santos C.A.M. Cordeiro M.V.S. Couto P.E.G. Paixão R.Y. Fujimoto N.C. Sousa In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish Arquivo Brasileiro de Medicina Veterinária e Zootecnia Amazon ornamental fish autochthonous probiotic in vitro assay |
| title | In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish |
| title_full | In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish |
| title_fullStr | In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish |
| title_full_unstemmed | In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish |
| title_short | In vitro bacterial probiotic selection from Nannostomus beckfordi, an Amazon ornamental fish |
| title_sort | in vitro bacterial probiotic selection from nannostomus beckfordi an amazon ornamental fish |
| topic | Amazon ornamental fish autochthonous probiotic in vitro assay |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000100111&tlng=en |
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