Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan
Background: Pseudomonas aeruginosa is a nosocomial pathogen which especially causes infections among burn patients. Carbapenems are extensively used for the treatment of infections caused by multidrug-resistant P. aeruginosa isolates. The emergence of carbapenemases producing isolates is an outcome...
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Alborz University of Medical Sciencs
2016-02-01
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| Series: | Bihdād |
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| Online Access: | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-129&slc_lang=en&sid=1 |
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| author | M. Pourbabaee F. Firoozeh M. Saffari M. Zibaei M. Sehat M. Radan |
| author_facet | M. Pourbabaee F. Firoozeh M. Saffari M. Zibaei M. Sehat M. Radan |
| author_sort | M. Pourbabaee |
| collection | DOAJ |
| description | Background: Pseudomonas aeruginosa is a nosocomial pathogen which especially causes infections among burn patients. Carbapenems are extensively used for the treatment of infections caused by multidrug-resistant P. aeruginosa isolates. The emergence of carbapenemases producing isolates is an outcome of increased utilization of carbapenems. The aim of this study was to determine the bla-IMP-1 and bla-IMP-2 genes in metallo-β-lactamase (MBL) -producing Pseudomonas aeruginosa isolated from burn patients in Isfahan.
Material and Methods: A total of 150 P. aeruginosa were isolated from burn patients hospitalized in Imam-Mousakazem hospital in Isfahan. Antimicrobial susceptibility was determined using disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Double Disk Synergy Test (DDST) was carried out for screening of MBL production in imipenem-resistant strains. PCR assays were used for detection of bla-IMP-1 and bla-IMP-2 genes among metallo-β-lactamase-producing Pseudomonas aeruginosa isolates. The purified PCR products were sequenced.
Results: Of 150 Pseudomonas aeruginosa isolates, %100 identified as multi-drug resistant strains. The most resistance rates were seen against ciprofloxacin, tobromycin, meropenem and imipenem. All of 144 imipenem-resistant Pseudomonas aeruginosa isolates were MBL producing by DDST test. Twenty-nine (19.3%) and 8(5.3%) MBL producing Pseudomonas aeruginosa isolates harbored bla-IMP-1 and bla-IMP-2 genes respectively.
Conclusions: According to results of this study high level resistance to imipenem and MBl genes carriage was seen among Pseudomonas aeruginosa isolated from burn patient infections in our region. |
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| institution | Directory Open Access Journal |
| issn | 1735-6679 |
| language | fas |
| last_indexed | 2024-12-20T03:17:15Z |
| publishDate | 2016-02-01 |
| publisher | Alborz University of Medical Sciencs |
| record_format | Article |
| series | Bihdād |
| spelling | doaj.art-169ef23c3b6b4d53bf9f40911dac763e2022-12-21T19:55:18ZfasAlborz University of Medical SciencsBihdād1735-66792016-02-015117Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in IsfahanM. Pourbabaee0F. Firoozeh1M. Saffari2M. Zibaei3M. Sehat4M. Radan5 Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan,. Iran Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan,. Iran Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan,. Iran Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Trauma Research Center, Kashan University of Medical Sciences, Kashan, Iran Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan,. Iran Background: Pseudomonas aeruginosa is a nosocomial pathogen which especially causes infections among burn patients. Carbapenems are extensively used for the treatment of infections caused by multidrug-resistant P. aeruginosa isolates. The emergence of carbapenemases producing isolates is an outcome of increased utilization of carbapenems. The aim of this study was to determine the bla-IMP-1 and bla-IMP-2 genes in metallo-β-lactamase (MBL) -producing Pseudomonas aeruginosa isolated from burn patients in Isfahan. Material and Methods: A total of 150 P. aeruginosa were isolated from burn patients hospitalized in Imam-Mousakazem hospital in Isfahan. Antimicrobial susceptibility was determined using disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Double Disk Synergy Test (DDST) was carried out for screening of MBL production in imipenem-resistant strains. PCR assays were used for detection of bla-IMP-1 and bla-IMP-2 genes among metallo-β-lactamase-producing Pseudomonas aeruginosa isolates. The purified PCR products were sequenced. Results: Of 150 Pseudomonas aeruginosa isolates, %100 identified as multi-drug resistant strains. The most resistance rates were seen against ciprofloxacin, tobromycin, meropenem and imipenem. All of 144 imipenem-resistant Pseudomonas aeruginosa isolates were MBL producing by DDST test. Twenty-nine (19.3%) and 8(5.3%) MBL producing Pseudomonas aeruginosa isolates harbored bla-IMP-1 and bla-IMP-2 genes respectively. Conclusions: According to results of this study high level resistance to imipenem and MBl genes carriage was seen among Pseudomonas aeruginosa isolated from burn patient infections in our region.http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-129&slc_lang=en&sid=1P. aeruginosa Burn patients Metallo-β-lactamase bla-IMP-1 bla-IMP-2 |
| spellingShingle | M. Pourbabaee F. Firoozeh M. Saffari M. Zibaei M. Sehat M. Radan Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan Bihdād P. aeruginosa Burn patients Metallo-β-lactamase bla-IMP-1 bla-IMP-2 |
| title | Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan |
| title_full | Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan |
| title_fullStr | Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan |
| title_full_unstemmed | Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan |
| title_short | Detection of bla-IMP-1 and bla-IMP-2 Genes Among Metallo-β-lactamase-Producing Pseudomonas Aeruginosa Isolated from Burn Patients in Isfahan |
| title_sort | detection of bla imp 1 and bla imp 2 genes among metallo β lactamase producing pseudomonas aeruginosa isolated from burn patients in isfahan |
| topic | P. aeruginosa Burn patients Metallo-β-lactamase bla-IMP-1 bla-IMP-2 |
| url | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-129&slc_lang=en&sid=1 |
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