Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells
Snail2 has an important role in the epithelial-mesenchymal transition (EMT) and tumor metastasis. Here, we report that Snail2 is highly expressed during TGF-β induced EMT in HL-7702 cells. Additionally, overexpression of Snail2 successfully promotes the migration and invasion of these cells, both in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00752/full |
| _version_ | 1819019323078868992 |
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| author | Yue Hu Qing Nie Mingrui Dai Fangfang Chen Hui Wu Hui Wu |
| author_facet | Yue Hu Qing Nie Mingrui Dai Fangfang Chen Hui Wu Hui Wu |
| author_sort | Yue Hu |
| collection | DOAJ |
| description | Snail2 has an important role in the epithelial-mesenchymal transition (EMT) and tumor metastasis. Here, we report that Snail2 is highly expressed during TGF-β induced EMT in HL-7702 cells. Additionally, overexpression of Snail2 successfully promotes the migration and invasion of these cells, both in vitro and in a mouse model. Furthermore, our results show that HDAC1 and HDAC3 could suppress the Snail2 gene promoter. Moreover, we find that the acetylation of H3K4 and H3K56 are significantly reduced during the EMT process of liver HL-7702 cells. Thus, our results indicate that HDAC1 and HDAC3 epigenetically suppress the expression of Snail2 during the EMT of liver cells, revealing an opposing function of HDACs during the migration of malignant tumors. |
| first_indexed | 2024-12-21T03:33:29Z |
| format | Article |
| id | doaj.art-16a1e738b9df4556993a6afbf4f94780 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-21T03:33:29Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-16a1e738b9df4556993a6afbf4f947802022-12-21T19:17:24ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-08-01810.3389/fcell.2020.00752552934Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma CellsYue Hu0Qing Nie1Mingrui Dai2Fangfang Chen3Hui Wu4Hui Wu5Department of Gastrointestinal and Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Gastrointestinal and Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaNational Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, ChinaDepartment of Gastrointestinal and Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaNational Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, ChinaSnail2 has an important role in the epithelial-mesenchymal transition (EMT) and tumor metastasis. Here, we report that Snail2 is highly expressed during TGF-β induced EMT in HL-7702 cells. Additionally, overexpression of Snail2 successfully promotes the migration and invasion of these cells, both in vitro and in a mouse model. Furthermore, our results show that HDAC1 and HDAC3 could suppress the Snail2 gene promoter. Moreover, we find that the acetylation of H3K4 and H3K56 are significantly reduced during the EMT process of liver HL-7702 cells. Thus, our results indicate that HDAC1 and HDAC3 epigenetically suppress the expression of Snail2 during the EMT of liver cells, revealing an opposing function of HDACs during the migration of malignant tumors.https://www.frontiersin.org/article/10.3389/fcell.2020.00752/fullepithelial-mesenchymal transitionSnail2metastasishistone deacetylaseshepatocellular carcinoma |
| spellingShingle | Yue Hu Qing Nie Mingrui Dai Fangfang Chen Hui Wu Hui Wu Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells Frontiers in Cell and Developmental Biology epithelial-mesenchymal transition Snail2 metastasis histone deacetylases hepatocellular carcinoma |
| title | Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells |
| title_full | Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells |
| title_fullStr | Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells |
| title_full_unstemmed | Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells |
| title_short | Histone Deacetylases Inhibit the Snail2-Mediated EMT During Metastasis of Hepatocellular Carcinoma Cells |
| title_sort | histone deacetylases inhibit the snail2 mediated emt during metastasis of hepatocellular carcinoma cells |
| topic | epithelial-mesenchymal transition Snail2 metastasis histone deacetylases hepatocellular carcinoma |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00752/full |
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