Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101
Abstract Background Enterotoxigenic Escherichia coli (ETEC) is classically associated with acute secretory diarrhea, which induces 2 million people death in developing countries over a year, predominantly children in the first years of life. Previously, tannins (47.75%) were extracted from Galla Chi...
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-021-03261-x |
| _version_ | 1818617290256547840 |
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| author | Xu Song Yi Yang Junzhi Li Mengxue He Yuanfeng Zou Renyong Jia Lixia Li Juan Hang Min Cui Lu Bai Zhongqiong Yin |
| author_facet | Xu Song Yi Yang Junzhi Li Mengxue He Yuanfeng Zou Renyong Jia Lixia Li Juan Hang Min Cui Lu Bai Zhongqiong Yin |
| author_sort | Xu Song |
| collection | DOAJ |
| description | Abstract Background Enterotoxigenic Escherichia coli (ETEC) is classically associated with acute secretory diarrhea, which induces 2 million people death in developing countries over a year, predominantly children in the first years of life. Previously, tannins (47.75%) were extracted from Galla Chinensis and prepared as Galla Chinensis oral solution (GOS) which showed significant antidiarrheal activity in a castor oil-induced diarrhea in mice. Whether the tannins extract were also effective in treatment of ETEC-induced diarrhea was determined in this study. Methods Mice were randomly divided into 6 groups (n = 22). The mice in the normal and untreated groups were given normal saline. Three GOS-treated groups were received different concentrations of GOS (5, 10 and 15%, respectively) at a dose of 10 mL/kg. Mice in the positive control group were fed with loperamide (10 mg/kg). The treatment with GOS started 3 days before infection with ETEC and continued for 4 consecutive days after infection. On day 3, mice were all infected with one dose of LD50 of ETEC, except those in the normal group. Survival of mice was observed daily and recorded throughout the study. On days 4 and 7, samples were collected from 6 mice in each group. Results GOS could increase the survival rate up to 75%, while in the untreated group it is 43.75%. The body weights of mice treated with 15% GOS were significantly increased on day 7 in comparison with the untreated group and the normal group. GOS-treatment recovered the small intestine coefficient enhanced by ETEC-infection. The diarrhea index of mice treated with GOS was significantly decreased. GOS increased the levels of IgG and sIgA in the terminal ileum and decreased the levels of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6 and IL-8) in serum. GOS could increase the amount of intestinal probiotics, Lactobacilli and Bifidobacteria. GOS could alleviate colon lesions induced by ETEC-infection. GOS showed higher potency than loperamide. Conclusions GOS could be a promising drug candidate for treating ETEC infections. |
| first_indexed | 2024-12-16T17:03:21Z |
| format | Article |
| id | doaj.art-16a204c533e24246ac873ebf14475a62 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-16T17:03:21Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-16a204c533e24246ac873ebf14475a622022-12-21T22:23:39ZengBMCBMC Complementary Medicine and Therapies2662-76712021-03-0121111010.1186/s12906-021-03261-xTannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101Xu Song0Yi Yang1Junzhi Li2Mengxue He3Yuanfeng Zou4Renyong Jia5Lixia Li6Juan Hang7Min Cui8Lu Bai9Zhongqiong Yin10Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityCollege of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityCollege of Veterinary Medicine, Sichuan Agricultural UniversityCollege of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural UniversityAbstract Background Enterotoxigenic Escherichia coli (ETEC) is classically associated with acute secretory diarrhea, which induces 2 million people death in developing countries over a year, predominantly children in the first years of life. Previously, tannins (47.75%) were extracted from Galla Chinensis and prepared as Galla Chinensis oral solution (GOS) which showed significant antidiarrheal activity in a castor oil-induced diarrhea in mice. Whether the tannins extract were also effective in treatment of ETEC-induced diarrhea was determined in this study. Methods Mice were randomly divided into 6 groups (n = 22). The mice in the normal and untreated groups were given normal saline. Three GOS-treated groups were received different concentrations of GOS (5, 10 and 15%, respectively) at a dose of 10 mL/kg. Mice in the positive control group were fed with loperamide (10 mg/kg). The treatment with GOS started 3 days before infection with ETEC and continued for 4 consecutive days after infection. On day 3, mice were all infected with one dose of LD50 of ETEC, except those in the normal group. Survival of mice was observed daily and recorded throughout the study. On days 4 and 7, samples were collected from 6 mice in each group. Results GOS could increase the survival rate up to 75%, while in the untreated group it is 43.75%. The body weights of mice treated with 15% GOS were significantly increased on day 7 in comparison with the untreated group and the normal group. GOS-treatment recovered the small intestine coefficient enhanced by ETEC-infection. The diarrhea index of mice treated with GOS was significantly decreased. GOS increased the levels of IgG and sIgA in the terminal ileum and decreased the levels of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6 and IL-8) in serum. GOS could increase the amount of intestinal probiotics, Lactobacilli and Bifidobacteria. GOS could alleviate colon lesions induced by ETEC-infection. GOS showed higher potency than loperamide. Conclusions GOS could be a promising drug candidate for treating ETEC infections.https://doi.org/10.1186/s12906-021-03261-xGalla ChinensisEnterotoxigenic Escherichia coliDiarrheaTannins |
| spellingShingle | Xu Song Yi Yang Junzhi Li Mengxue He Yuanfeng Zou Renyong Jia Lixia Li Juan Hang Min Cui Lu Bai Zhongqiong Yin Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101 BMC Complementary Medicine and Therapies Galla Chinensis Enterotoxigenic Escherichia coli Diarrhea Tannins |
| title | Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101 |
| title_full | Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101 |
| title_fullStr | Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101 |
| title_full_unstemmed | Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101 |
| title_short | Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101 |
| title_sort | tannins extract from galla chinensis can protect mice from infection by enterotoxigenic escherichia coli o101 |
| topic | Galla Chinensis Enterotoxigenic Escherichia coli Diarrhea Tannins |
| url | https://doi.org/10.1186/s12906-021-03261-x |
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