Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study
Introduction: Chronic Obstructive Pulmonary Disease (COPD) is characterized by partially reversible airflow obstruction and chronic inflammation in the pulmonary as well as in systemic compartment. Interleukin 8 (IL-8) is one of the most potent inflammatory mediators and it is involved in the mi...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2017-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/10849/28279_CE(RA1)_F(T)_PF1(PB_AP)_PFA(MJ_GG)_PF2(MJ_SS).pdf |
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| author | Prashant Mani Tripathi Surya Kant Ravi Shanker Yadav R.A.S. Kushwaha Ved Prakash Abbas Ali Mahdi Abhishek Dubey |
| author_facet | Prashant Mani Tripathi Surya Kant Ravi Shanker Yadav R.A.S. Kushwaha Ved Prakash Abbas Ali Mahdi Abhishek Dubey |
| author_sort | Prashant Mani Tripathi |
| collection | DOAJ |
| description | Introduction: Chronic Obstructive Pulmonary Disease (COPD)
is characterized by partially reversible airflow obstruction and
chronic inflammation in the pulmonary as well as in systemic
compartment. Interleukin 8 (IL-8) is one of the most potent
inflammatory mediators and it is involved in the migration and
activation of neutrophils. Previous studies demonstrated the
increased levels of IL-8 in plasma and sputum of COPD, but
peripheral blood neutrophils mediated IL-8 level including their
relationship to smoking pack years and spirometric parameters
is still unknown.
Aim: To assess the levels of IL-8 in sputum and peripheral blood
neutrophils of COPD patients and to compare them with healthy
controls.
Materials and Methods: This case control study was done
from March 2014 to November 2016 in the department of
respiratory medicine KGMU. A total of 81 COPD patients and
81 healthy controls were recruited in this study. Peripheral
blood neutrophils were isolated from COPD patients and
healthy controls and stimulated with Lipopolysaccharide (LPS).
The neutrophils cell culture supernatant was assessed for IL-8
level using ELISA techniques, whilst IL-8 mRNA expression was
measured using real-time polymerase chain reaction. Induced
sputum was obtained from all participants and processed. The
sputum supernatant was also assayed for IL-8 level.
Results: COPD patients had significantly higher protein level
of IL-8 in peripheral blood neutrophils (p=0.018) and sputum
supernatant (p=0.011) compared to healthy controls. Gene
expression of IL-8 was also significantly higher in peripheral
blood neutrophils of COPD patients (p=0.001). In the patients
with COPD, the smoke pack year was significantly correlated
with the level of IL-8 in peripheral blood neutrophils (r=0.42;
p=0.009) and sputum (r=0.48; p=0.002). The smoke pack years
was found negatively correlated with FEV1 % predicted (r=-
0.37; p=0.044) and FEV1 /FVC (r=-0.47; p=0.007) in the COPD
patients.
Conclusion: The increased level of IL-8 in peripheral blood
neutrophils and sputum support the hypothesis that COPD is a
multicomponent disease. IL-8 level may be helpful in predicting
development of COPD in smokers. |
| first_indexed | 2024-12-19T21:18:12Z |
| format | Article |
| id | doaj.art-16a7d4695ff34970b930edb9c75b3a8b |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-19T21:18:12Z |
| publishDate | 2017-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-16a7d4695ff34970b930edb9c75b3a8b2022-12-21T20:05:18ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-11-011111OC13OC1710.7860/JCDR/2017/28279.10849Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control StudyPrashant Mani Tripathi0Surya Kant1Ravi Shanker Yadav2R.A.S. Kushwaha3Ved Prakash4Abbas Ali Mahdi5Abhishek Dubey6Research Scholar, Department of Respiratory Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India.Professor and Head, Department of Respiratory Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India.Research Scholar, Department of Respiratory Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India.Professor, Department of Respiratory Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India.Assistant Professor, Department of Respiratory Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India.Professor and Head, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, India.Research Scholar, Department of Respiratory Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India.Introduction: Chronic Obstructive Pulmonary Disease (COPD) is characterized by partially reversible airflow obstruction and chronic inflammation in the pulmonary as well as in systemic compartment. Interleukin 8 (IL-8) is one of the most potent inflammatory mediators and it is involved in the migration and activation of neutrophils. Previous studies demonstrated the increased levels of IL-8 in plasma and sputum of COPD, but peripheral blood neutrophils mediated IL-8 level including their relationship to smoking pack years and spirometric parameters is still unknown. Aim: To assess the levels of IL-8 in sputum and peripheral blood neutrophils of COPD patients and to compare them with healthy controls. Materials and Methods: This case control study was done from March 2014 to November 2016 in the department of respiratory medicine KGMU. A total of 81 COPD patients and 81 healthy controls were recruited in this study. Peripheral blood neutrophils were isolated from COPD patients and healthy controls and stimulated with Lipopolysaccharide (LPS). The neutrophils cell culture supernatant was assessed for IL-8 level using ELISA techniques, whilst IL-8 mRNA expression was measured using real-time polymerase chain reaction. Induced sputum was obtained from all participants and processed. The sputum supernatant was also assayed for IL-8 level. Results: COPD patients had significantly higher protein level of IL-8 in peripheral blood neutrophils (p=0.018) and sputum supernatant (p=0.011) compared to healthy controls. Gene expression of IL-8 was also significantly higher in peripheral blood neutrophils of COPD patients (p=0.001). In the patients with COPD, the smoke pack year was significantly correlated with the level of IL-8 in peripheral blood neutrophils (r=0.42; p=0.009) and sputum (r=0.48; p=0.002). The smoke pack years was found negatively correlated with FEV1 % predicted (r=- 0.37; p=0.044) and FEV1 /FVC (r=-0.47; p=0.007) in the COPD patients. Conclusion: The increased level of IL-8 in peripheral blood neutrophils and sputum support the hypothesis that COPD is a multicomponent disease. IL-8 level may be helpful in predicting development of COPD in smokers.https://jcdr.net/articles/PDF/10849/28279_CE(RA1)_F(T)_PF1(PB_AP)_PFA(MJ_GG)_PF2(MJ_SS).pdfairflow obstructioninflammationlipopolysaccharide |
| spellingShingle | Prashant Mani Tripathi Surya Kant Ravi Shanker Yadav R.A.S. Kushwaha Ved Prakash Abbas Ali Mahdi Abhishek Dubey Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study Journal of Clinical and Diagnostic Research airflow obstruction inflammation lipopolysaccharide |
| title | Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study |
| title_full | Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study |
| title_fullStr | Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study |
| title_full_unstemmed | Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study |
| title_short | Effect of Chronic Obstructive Pulmonary Disease on Interleukin-8 Level in Sputum and Peripheral Blood Neutrophils: A Case Control Study |
| title_sort | effect of chronic obstructive pulmonary disease on interleukin 8 level in sputum and peripheral blood neutrophils a case control study |
| topic | airflow obstruction inflammation lipopolysaccharide |
| url | https://jcdr.net/articles/PDF/10849/28279_CE(RA1)_F(T)_PF1(PB_AP)_PFA(MJ_GG)_PF2(MJ_SS).pdf |
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