An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Abstract Background A neutral, heat-sensitive serine protease (NHSSP) originating from the feather-degrading fungus Onygena corvina (O. corvina) was described and defined as an alkaline serine protease of the subtilisin type S8 family, exhibiting an enzymatic activity at neutral pH. Generally, broad...

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Main Authors: Piotr M. Skowron, Daria Krefft, Robert Brodzik, Paulina Kasperkiewicz, Marcin Drag, Klaus-Peter Koller
格式: 文件
语言:English
出版: BMC 2020-06-01
丛编:Microbial Cell Factories
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在线阅读:http://link.springer.com/article/10.1186/s12934-020-01392-3