Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage
The acid mine drainage (AMD) impacted creek of the Carnoulès mine (Southern France) is characterized by acid waters with a high heavy metal content. The microbial community inhabiting this AMD was extensively studied using isolation, metagenomic and metaproteomic methods, and the results showed that...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00003/full |
| _version_ | 1818264578575826944 |
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| author | Agnès eHovasse Odile eBruneel Corinne eCasiot Angélique eDesoeuvre Julien eFarasin Marina eHery Alain eVan Dorsselaer Christine eCarapito Florence eArsene-Ploetze |
| author_facet | Agnès eHovasse Odile eBruneel Corinne eCasiot Angélique eDesoeuvre Julien eFarasin Marina eHery Alain eVan Dorsselaer Christine eCarapito Florence eArsene-Ploetze |
| author_sort | Agnès eHovasse |
| collection | DOAJ |
| description | The acid mine drainage (AMD) impacted creek of the Carnoulès mine (Southern France) is characterized by acid waters with a high heavy metal content. The microbial community inhabiting this AMD was extensively studied using isolation, metagenomic and metaproteomic methods, and the results showed that a natural arsenic (and iron) attenuation process involving the arsenite oxidase activity of several Thiomonas strains occurs at this site. A sensitive quantitative Selected Reaction Monitoring (SRM)-based proteomic approach was developed for detecting and quantifying the two subunits of the arsenite oxidase and RpoA of two different Thiomonas groups. Using this approach combined with 16S rRNA gene sequence analysis based on pyrosequencing and FISH, it was established here for the first time that these Thiomonas strains are ubiquitously present in minor proportions in this AMD and that they express the key enzymes involved in natural remediation processes at various locations and time points. In addition to these findings, this study also confirms that targeted proteomics applied at the community level can be used to detect weakly abundant proteins in situ. |
| first_indexed | 2024-12-12T19:37:09Z |
| format | Article |
| id | doaj.art-16abb72154554bf99522dd14ae535b8d |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-12T19:37:09Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-16abb72154554bf99522dd14ae535b8d2022-12-22T00:14:17ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2016-02-01410.3389/fcell.2016.00003169472Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine DrainageAgnès eHovasse0Odile eBruneel1Corinne eCasiot2Angélique eDesoeuvre3Julien eFarasin4Marina eHery5Alain eVan Dorsselaer6Christine eCarapito7Florence eArsene-Ploetze8IPHC, Université de Strasbourg, CNRS,CNRS, Université Montpellier, IRDCNRS, Université Montpellier, IRDCNRS, Université Montpellier, IRDUniversité de Strasbourg, CNRSCNRS, Université Montpellier, IRDIPHC, Université de Strasbourg, CNRS,IPHC, Université de Strasbourg, CNRS,Université de Strasbourg, CNRSThe acid mine drainage (AMD) impacted creek of the Carnoulès mine (Southern France) is characterized by acid waters with a high heavy metal content. The microbial community inhabiting this AMD was extensively studied using isolation, metagenomic and metaproteomic methods, and the results showed that a natural arsenic (and iron) attenuation process involving the arsenite oxidase activity of several Thiomonas strains occurs at this site. A sensitive quantitative Selected Reaction Monitoring (SRM)-based proteomic approach was developed for detecting and quantifying the two subunits of the arsenite oxidase and RpoA of two different Thiomonas groups. Using this approach combined with 16S rRNA gene sequence analysis based on pyrosequencing and FISH, it was established here for the first time that these Thiomonas strains are ubiquitously present in minor proportions in this AMD and that they express the key enzymes involved in natural remediation processes at various locations and time points. In addition to these findings, this study also confirms that targeted proteomics applied at the community level can be used to detect weakly abundant proteins in situ.http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00003/fullpyrosequencingfishacid mine drainagetargeted proteomicsCommunity proteomicsLC-SRM |
| spellingShingle | Agnès eHovasse Odile eBruneel Corinne eCasiot Angélique eDesoeuvre Julien eFarasin Marina eHery Alain eVan Dorsselaer Christine eCarapito Florence eArsene-Ploetze Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage Frontiers in Cell and Developmental Biology pyrosequencing fish acid mine drainage targeted proteomics Community proteomics LC-SRM |
| title | Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage |
| title_full | Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage |
| title_fullStr | Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage |
| title_full_unstemmed | Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage |
| title_short | Spatio-temporal detection of the Thiomonas population and the Thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the Carnoulès Acid Mine Drainage |
| title_sort | spatio temporal detection of the thiomonas population and the thiomonas arsenite oxidase involved in natural arsenite attenuation processes in the carnoul 232 s acid mine drainage |
| topic | pyrosequencing fish acid mine drainage targeted proteomics Community proteomics LC-SRM |
| url | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00003/full |
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