Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids
Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Debrecen
1999-09-01
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| Series: | International Journal of Horticultural Science |
| Subjects: | |
| Online Access: | https://ojs.lib.unideb.hu/IJHS/article/view/41 |
| _version_ | 1818054903957815296 |
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| author | A. M. Fontes C. W. Otoni S. V. Maia H. S. Brommonschenkel M. Fári |
| author_facet | A. M. Fontes C. W. Otoni S. V. Maia H. S. Brommonschenkel M. Fári |
| author_sort | A. M. Fontes |
| collection | DOAJ |
| description | Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated. |
| first_indexed | 2024-12-10T12:04:27Z |
| format | Article |
| id | doaj.art-16ad8227f6d346a396286268c1b48acc |
| institution | Directory Open Access Journal |
| issn | 1585-0404 2676-931X |
| language | English |
| last_indexed | 2024-12-10T12:04:27Z |
| publishDate | 1999-09-01 |
| publisher | University of Debrecen |
| record_format | Article |
| series | International Journal of Horticultural Science |
| spelling | doaj.art-16ad8227f6d346a396286268c1b48acc2022-12-22T01:49:31ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X1999-09-0153-410.31421/IJHS/5/3-4/41Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybridsA. M. Fontes0C. W. Otoni1S. V. Maia2H. S. Brommonschenkel3M. Fári4Departamento de Biologia Vegetal Av. P. H. Rolphs, sln, Campus Universitario, 36571-000, Vicosa, MG, Brazil; Universidade Federal de Vicosa, Vicosa, MG, BrazilDepartamento de Biologia Vegetal and BIOAGRO, Av. P. H. Rolphs, sln, Campus Universitario, 36571-000, Vicosa, MG, Brazil; Universidade Federal de Vicosa, Vicosa, MG, BrazilBIOAGRO, Av. P. H. Rolphs, sln, Campus Universitario, 36571-000, Vicosa, MG, Brazil; Universidade Federal de Vicosa, Vicosa, MG, Brazil.BIOAGRO, Av. P. H. Rolphs, sln, Campus Universitario, 36571-000, Vicosa, MG, Brazil; Universidade Federal de Vicosa, Vicosa, MG, Brazil.Consultant, Codevasf /Embrapa/ Agroinvest, Embrapa Semi-Arido (CPATSA), Br 428, km 152, Zona Rural, Petrolina—PECotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated.https://ojs.lib.unideb.hu/IJHS/article/view/41Capsicum annuumBrazilian and Hungarian cultivarsprotoplast isolationalginate embeddingmicrocolony formationcallus formation |
| spellingShingle | A. M. Fontes C. W. Otoni S. V. Maia H. S. Brommonschenkel M. Fári Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids International Journal of Horticultural Science Capsicum annuum Brazilian and Hungarian cultivars protoplast isolation alginate embedding microcolony formation callus formation |
| title | Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids |
| title_full | Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids |
| title_fullStr | Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids |
| title_full_unstemmed | Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids |
| title_short | Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids |
| title_sort | comparative investigations on protoplast culture of some brazilian and hungarian sweet pepper cultivars and hybrids |
| topic | Capsicum annuum Brazilian and Hungarian cultivars protoplast isolation alginate embedding microcolony formation callus formation |
| url | https://ojs.lib.unideb.hu/IJHS/article/view/41 |
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