Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses
IntroductionPlant-specific Class III peroxidases (PRXs) play a crucial role in lignification, cell elongation, seed germination, and biotic and abiotic stresses.MethodsThe class III peroxidase gene family in sugarcane were identified by bioinformatics methods and realtime fluorescence quantitative P...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1101665/full |
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| author | Heyang Shang Heyang Shang Linqi Fang Lifang Qin Hongtao Jiang Hongtao Jiang Zhenzhen Duan Hai Zhang Zongtao Yang Guangyuan Cheng Yixue Bao Jingsheng Xu Jingsheng Xu Wei Yao Muqing Zhang Muqing Zhang |
| author_facet | Heyang Shang Heyang Shang Linqi Fang Lifang Qin Hongtao Jiang Hongtao Jiang Zhenzhen Duan Hai Zhang Zongtao Yang Guangyuan Cheng Yixue Bao Jingsheng Xu Jingsheng Xu Wei Yao Muqing Zhang Muqing Zhang |
| author_sort | Heyang Shang |
| collection | DOAJ |
| description | IntroductionPlant-specific Class III peroxidases (PRXs) play a crucial role in lignification, cell elongation, seed germination, and biotic and abiotic stresses.MethodsThe class III peroxidase gene family in sugarcane were identified by bioinformatics methods and realtime fluorescence quantitative PCR.ResultsEighty-two PRX proteins were characterized with a conserved PRX domain as members of the class III PRX gene family in R570 STP. The ShPRX family genes were divided into six groups by the phylogenetic analysis of sugarcane, Saccharum spontaneum, sorghum, rice, and Arabidopsis thaliana. The analysis of promoter cis-acting elements revealed that most ShPRX family genes contained cis-acting regulatory elements involved in ABA, MeJA, light responsiveness, anaerobic induction, and drought inducibility. An evolutionary analysis indicated that ShPRXs was formed after Poaceae and Bromeliaceae diverged, and tandem duplication events played a critical role in the expansion of ShPRX genes of sugarcane. Purifying selection maintained the function of ShPRX proteins. SsPRX genes were differentially expressed in stems and leaves at different growth stages in S. spontaneum. However, ShPRX genes were differentially expressed in the SCMV-inoculated sugarcane plants. A qRT-PCR analysis showed that SCMV, Cd, and salt could specifically induce the expression of PRX genes of sugarcane.DiscussionThese results help elucidate the structure, evolution, and functions of the class III PRX gene family in sugarcane and provide ideas for the phytoremediation of Cd-contaminated soil and breeding new sugarcane varieties resistant to sugarcane mosaic disease, salt, and Cd stresses. |
| first_indexed | 2024-04-10T19:10:41Z |
| format | Article |
| id | doaj.art-16aeaef2228c494aafceb78a67437037 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T19:10:41Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-16aeaef2228c494aafceb78a674370372023-01-30T15:58:05ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011410.3389/fpls.2023.11016651101665Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stressesHeyang Shang0Heyang Shang1Linqi Fang2Lifang Qin3Hongtao Jiang4Hongtao Jiang5Zhenzhen Duan6Hai Zhang7Zongtao Yang8Guangyuan Cheng9Yixue Bao10Jingsheng Xu11Jingsheng Xu12Wei Yao13Muqing Zhang14Muqing Zhang15National Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources & Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, ChinaIntroductionPlant-specific Class III peroxidases (PRXs) play a crucial role in lignification, cell elongation, seed germination, and biotic and abiotic stresses.MethodsThe class III peroxidase gene family in sugarcane were identified by bioinformatics methods and realtime fluorescence quantitative PCR.ResultsEighty-two PRX proteins were characterized with a conserved PRX domain as members of the class III PRX gene family in R570 STP. The ShPRX family genes were divided into six groups by the phylogenetic analysis of sugarcane, Saccharum spontaneum, sorghum, rice, and Arabidopsis thaliana. The analysis of promoter cis-acting elements revealed that most ShPRX family genes contained cis-acting regulatory elements involved in ABA, MeJA, light responsiveness, anaerobic induction, and drought inducibility. An evolutionary analysis indicated that ShPRXs was formed after Poaceae and Bromeliaceae diverged, and tandem duplication events played a critical role in the expansion of ShPRX genes of sugarcane. Purifying selection maintained the function of ShPRX proteins. SsPRX genes were differentially expressed in stems and leaves at different growth stages in S. spontaneum. However, ShPRX genes were differentially expressed in the SCMV-inoculated sugarcane plants. A qRT-PCR analysis showed that SCMV, Cd, and salt could specifically induce the expression of PRX genes of sugarcane.DiscussionThese results help elucidate the structure, evolution, and functions of the class III PRX gene family in sugarcane and provide ideas for the phytoremediation of Cd-contaminated soil and breeding new sugarcane varieties resistant to sugarcane mosaic disease, salt, and Cd stresses.https://www.frontiersin.org/articles/10.3389/fpls.2023.1101665/fullsugarcaneclass III peroxidasesugarcane mosaic viruscadmiumsalt stress |
| spellingShingle | Heyang Shang Heyang Shang Linqi Fang Lifang Qin Hongtao Jiang Hongtao Jiang Zhenzhen Duan Hai Zhang Zongtao Yang Guangyuan Cheng Yixue Bao Jingsheng Xu Jingsheng Xu Wei Yao Muqing Zhang Muqing Zhang Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses Frontiers in Plant Science sugarcane class III peroxidase sugarcane mosaic virus cadmium salt stress |
| title | Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses |
| title_full | Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses |
| title_fullStr | Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses |
| title_full_unstemmed | Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses |
| title_short | Genome-wide identification of the class III peroxidase gene family of sugarcane and its expression profiles under stresses |
| title_sort | genome wide identification of the class iii peroxidase gene family of sugarcane and its expression profiles under stresses |
| topic | sugarcane class III peroxidase sugarcane mosaic virus cadmium salt stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1101665/full |
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