Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test
The dorsal raphe nucleus (DRN) is a major source of serotonin in the central nervous system, which is closely related to depression-like behaviors and is modulated by local GABAergic interneurons. Although serotonin neurons are known to be activated by struggling behavior in tail suspension test (TS...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.893465/full |
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| author | Liuchang Zhou Liuchang Zhou Dan Liu Zedan Xie Di Deng Di Deng Guoqi Shi Jinlan Zhao Shasha Bai Lei Yang Rong Zhang Yafei Shi |
| author_facet | Liuchang Zhou Liuchang Zhou Dan Liu Zedan Xie Di Deng Di Deng Guoqi Shi Jinlan Zhao Shasha Bai Lei Yang Rong Zhang Yafei Shi |
| author_sort | Liuchang Zhou |
| collection | DOAJ |
| description | The dorsal raphe nucleus (DRN) is a major source of serotonin in the central nervous system, which is closely related to depression-like behaviors and is modulated by local GABAergic interneurons. Although serotonin neurons are known to be activated by struggling behavior in tail suspension test (TST), the exact electrophysiological characteristics are still unclear. Here, we combined in vivo electrode recording and behavioral test to explore the mice neuron electrophysiology in DRN during TST and observed that gamma oscillation was related to despair-like behaviors whereas burst fraction was crucial for survival-like behaviors. We reported the identification of a subpopulation of DRN neurons which change their firing rates when mice get into and during TST immobile states. Both increase (putative despair units, D units for short) and decrease (putative survival units, S units for short) in firing rate were observed. Furthermore, using optogenetics to identify parvalbumin-positive (PV+) and serotonin transporter-positive (SERT+) neurons, we found that SERT+ neurons were almost S units. Interestingly, those that have been identified PV+ neurons include ~20% of D units and ~50% of S units. These results suggest that electrophysiological characteristics incorporated in despair-like behavior studies can provide new insight into the study of anti-depression targets, and GABAergic interneuron is a complex key hub to the coding and regulation of local neural network. |
| first_indexed | 2024-04-12T10:50:46Z |
| format | Article |
| id | doaj.art-16b4556434de48e5a43e83796af11dbd |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-12T10:50:46Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-16b4556434de48e5a43e83796af11dbd2022-12-22T03:36:15ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532022-05-011610.3389/fnbeh.2022.893465893465Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension TestLiuchang Zhou0Liuchang Zhou1Dan Liu2Zedan Xie3Di Deng4Di Deng5Guoqi Shi6Jinlan Zhao7Shasha Bai8Lei Yang9Rong Zhang10Yafei Shi11School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, ChinaSchool of Foreign Studies, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaJoint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education, International Institute for Translational Chinese Medicine, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Fundamental Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe dorsal raphe nucleus (DRN) is a major source of serotonin in the central nervous system, which is closely related to depression-like behaviors and is modulated by local GABAergic interneurons. Although serotonin neurons are known to be activated by struggling behavior in tail suspension test (TST), the exact electrophysiological characteristics are still unclear. Here, we combined in vivo electrode recording and behavioral test to explore the mice neuron electrophysiology in DRN during TST and observed that gamma oscillation was related to despair-like behaviors whereas burst fraction was crucial for survival-like behaviors. We reported the identification of a subpopulation of DRN neurons which change their firing rates when mice get into and during TST immobile states. Both increase (putative despair units, D units for short) and decrease (putative survival units, S units for short) in firing rate were observed. Furthermore, using optogenetics to identify parvalbumin-positive (PV+) and serotonin transporter-positive (SERT+) neurons, we found that SERT+ neurons were almost S units. Interestingly, those that have been identified PV+ neurons include ~20% of D units and ~50% of S units. These results suggest that electrophysiological characteristics incorporated in despair-like behavior studies can provide new insight into the study of anti-depression targets, and GABAergic interneuron is a complex key hub to the coding and regulation of local neural network.https://www.frontiersin.org/articles/10.3389/fnbeh.2022.893465/fullin vivo electrophysiologytail suspension testdorsal raphe nucleusserotonergic (5-HT) nucleidespair-like behavior |
| spellingShingle | Liuchang Zhou Liuchang Zhou Dan Liu Zedan Xie Di Deng Di Deng Guoqi Shi Jinlan Zhao Shasha Bai Lei Yang Rong Zhang Yafei Shi Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test Frontiers in Behavioral Neuroscience in vivo electrophysiology tail suspension test dorsal raphe nucleus serotonergic (5-HT) nuclei despair-like behavior |
| title | Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test |
| title_full | Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test |
| title_fullStr | Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test |
| title_full_unstemmed | Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test |
| title_short | Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test |
| title_sort | electrophysiological characteristics of dorsal raphe nucleus in tail suspension test |
| topic | in vivo electrophysiology tail suspension test dorsal raphe nucleus serotonergic (5-HT) nuclei despair-like behavior |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.893465/full |
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