Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain
Porcine epidemic diarrhea virus (PEDV), which re-emerged in China in 2010, has caused severe economic losses to the global pig industry. In this study, a PEDV strain, designated PEDV WMB, was isolated from piglets with severe diarrhea on a pig farm in Henan Province of China. Whole-genome sequencing...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1203893/full |
| _version_ | 1797824420559978496 |
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| author | Xueying Han Yangkun Liu Yan Wang Tiejun Wang Ning Li Feng Hao Lunguang Yao Kangkang Guo |
| author_facet | Xueying Han Yangkun Liu Yan Wang Tiejun Wang Ning Li Feng Hao Lunguang Yao Kangkang Guo |
| author_sort | Xueying Han |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV), which re-emerged in China in 2010, has caused severe economic losses to the global pig industry. In this study, a PEDV strain, designated PEDV WMB, was isolated from piglets with severe diarrhea on a pig farm in Henan Province of China. Whole-genome sequencing and analysis revealed that the PEDV WMB strain belongs to subtype G2c and has a unique continuous mutation in the S10 antigenic epitope of the S protein. Moreover, the virus-neutralization (VN) test indicated that polyclonal antibodies against the S10 protein of other G1 and G2 strains showed reduced VN reactivity to PEDV WMB. The pathogenicity of PEDV WMB was further investigated in 3 day-old piglets. PEDV infection-related clinical symptoms and morphological lesions were observed and confirmed by histopathological and immunohistochemical examination (IHC). These results illustrated that continuous mutation of the S10 epitope might affect the immunogenicity or pathogenicity of PEDV, providing evidence of the need to monitor the genetic diversity of the virus and develop effective measures to prevent and control PEDV. |
| first_indexed | 2024-03-13T10:38:42Z |
| format | Article |
| id | doaj.art-16b47c2aa8be4a6f985ea52c0cc29dec |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T10:38:42Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-16b47c2aa8be4a6f985ea52c0cc29dec2023-05-18T05:09:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.12038931203893Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domainXueying Han0Yangkun Liu1Yan Wang2Tiejun Wang3Ning Li4Feng Hao5Lunguang Yao6Kangkang Guo7College of Veterinary Medicine, Northwest A&F University, Shaanxi, ChinaHenan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, ChinaCenter of Advanced Analysis & Gene Sequencing, Zhengzhou University, Zhengzhou, Henan, ChinaHenan Province Engineering Technology Research Center of Animal Disease Control and Prevention, Nanyang Vocational College of Agriculture, Nanyang, Henan, ChinaCollege of Veterinary Medicine, Northwest A&F University, Shaanxi, ChinaCollege of Veterinary Medicine, Northwest A&F University, Shaanxi, ChinaHenan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, ChinaCollege of Veterinary Medicine, Northwest A&F University, Shaanxi, ChinaPorcine epidemic diarrhea virus (PEDV), which re-emerged in China in 2010, has caused severe economic losses to the global pig industry. In this study, a PEDV strain, designated PEDV WMB, was isolated from piglets with severe diarrhea on a pig farm in Henan Province of China. Whole-genome sequencing and analysis revealed that the PEDV WMB strain belongs to subtype G2c and has a unique continuous mutation in the S10 antigenic epitope of the S protein. Moreover, the virus-neutralization (VN) test indicated that polyclonal antibodies against the S10 protein of other G1 and G2 strains showed reduced VN reactivity to PEDV WMB. The pathogenicity of PEDV WMB was further investigated in 3 day-old piglets. PEDV infection-related clinical symptoms and morphological lesions were observed and confirmed by histopathological and immunohistochemical examination (IHC). These results illustrated that continuous mutation of the S10 epitope might affect the immunogenicity or pathogenicity of PEDV, providing evidence of the need to monitor the genetic diversity of the virus and develop effective measures to prevent and control PEDV.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1203893/fullporcine epidemic diarrhea virusS10 domaincontinuous mutationpathogenicityisolation |
| spellingShingle | Xueying Han Yangkun Liu Yan Wang Tiejun Wang Ning Li Feng Hao Lunguang Yao Kangkang Guo Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain Frontiers in Microbiology porcine epidemic diarrhea virus S10 domain continuous mutation pathogenicity isolation |
| title | Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain |
| title_full | Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain |
| title_fullStr | Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain |
| title_full_unstemmed | Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain |
| title_short | Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain |
| title_sort | isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the s10 domain |
| topic | porcine epidemic diarrhea virus S10 domain continuous mutation pathogenicity isolation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1203893/full |
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