Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i>
The efficient culture and purification of antimicrobial peptides (AMPs), along with intense antioxidant activity, have drawn the interest to study antioxidant activity mechanism. We report the culture conditions optimization, efficient biosynthesis, and purification of an antioxidant peptide MS15 fr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/10/934 |
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| author | Md Maruf Khan Young Kyun Kim Tahmina Bilkis Joo-Won Suh Dae Young Lee Jin Cheol Yoo |
| author_facet | Md Maruf Khan Young Kyun Kim Tahmina Bilkis Joo-Won Suh Dae Young Lee Jin Cheol Yoo |
| author_sort | Md Maruf Khan |
| collection | DOAJ |
| description | The efficient culture and purification of antimicrobial peptides (AMPs), along with intense antioxidant activity, have drawn the interest to study antioxidant activity mechanism. We report the culture conditions optimization, efficient biosynthesis, and purification of an antioxidant peptide MS15 from <i>Bacillus velezensis</i> obtained from fermented food that would generate heme oxygenase-1 (HO-1) expression and lead to nuclear factor erythroid 2-related factor-2 (Nrf2) nuclear translocation. We explored the ability of kinetics and potency for the bacterial killing to work against various pathogenic bacteria. A bioassay showed the lysis zone of MS15 by tricine SDS-PAGE near at 6 kDa. MALDI-TOF/MS verified molecular weight, and the existence of a molecular mass of 6091 Da was reported by purity. The MIC of MS15 ranged from 2.5–160 μg/mL for many pathogenic bacteria, showing greater potency. In macrophage RAW 264.7 cells, MS15 was exposed to assess its inhibitory effect against the generation of reactive oxygen species (ROS) in oxidative stress. In the sample treated group, the translation, and transcriptional levels of CAT (catalase), GPx (glutathione peroxidase), and SOD (superoxide dismutase) were significantly greater. In short, MS15 has significant antioxidant properties, reducing ROS production in RAW 264.7 cells, and raising the translation and transcriptional rates of antioxidant enzymes with stimulating HO-1 induction facilitated by Nrf2. |
| first_indexed | 2024-03-09T06:05:58Z |
| format | Article |
| id | doaj.art-16baa754f27a481e9c82057a0d33e2ed |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T06:05:58Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-16baa754f27a481e9c82057a0d33e2ed2023-12-03T12:02:44ZengMDPI AGAntioxidants2076-39212020-09-0191093410.3390/antiox9100934Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i>Md Maruf Khan0Young Kyun Kim1Tahmina Bilkis2Joo-Won Suh3Dae Young Lee4Jin Cheol Yoo5Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, KoreaDepartment of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, KoreaDepartment of Biomedical Sciences, Chosun University, Gwangju 501-759, KoreaCenter for Nutraceutical and Pharmaceutical Materials, Myongji University, Myongji-ro 116, Cheoin-gu, Yongin 17058, Gyeonggi-Do, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, KoreaThe efficient culture and purification of antimicrobial peptides (AMPs), along with intense antioxidant activity, have drawn the interest to study antioxidant activity mechanism. We report the culture conditions optimization, efficient biosynthesis, and purification of an antioxidant peptide MS15 from <i>Bacillus velezensis</i> obtained from fermented food that would generate heme oxygenase-1 (HO-1) expression and lead to nuclear factor erythroid 2-related factor-2 (Nrf2) nuclear translocation. We explored the ability of kinetics and potency for the bacterial killing to work against various pathogenic bacteria. A bioassay showed the lysis zone of MS15 by tricine SDS-PAGE near at 6 kDa. MALDI-TOF/MS verified molecular weight, and the existence of a molecular mass of 6091 Da was reported by purity. The MIC of MS15 ranged from 2.5–160 μg/mL for many pathogenic bacteria, showing greater potency. In macrophage RAW 264.7 cells, MS15 was exposed to assess its inhibitory effect against the generation of reactive oxygen species (ROS) in oxidative stress. In the sample treated group, the translation, and transcriptional levels of CAT (catalase), GPx (glutathione peroxidase), and SOD (superoxide dismutase) were significantly greater. In short, MS15 has significant antioxidant properties, reducing ROS production in RAW 264.7 cells, and raising the translation and transcriptional rates of antioxidant enzymes with stimulating HO-1 induction facilitated by Nrf2.https://www.mdpi.com/2076-3921/9/10/934peptide MS15oxidative stressROS (reactive oxygen species)antioxidantNrf2/HO-1 (Nuclear factor erythroid 2-related factor-2/heme oxygenase-1) |
| spellingShingle | Md Maruf Khan Young Kyun Kim Tahmina Bilkis Joo-Won Suh Dae Young Lee Jin Cheol Yoo Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i> Antioxidants peptide MS15 oxidative stress ROS (reactive oxygen species) antioxidant Nrf2/HO-1 (Nuclear factor erythroid 2-related factor-2/heme oxygenase-1) |
| title | Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i> |
| title_full | Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i> |
| title_fullStr | Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i> |
| title_full_unstemmed | Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i> |
| title_short | Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from <i>Bacillus velezensis</i> |
| title_sort | reduction of oxidative stress through activating the nrf2 mediated ho 1 antioxidant efficacy signaling pathway by ms15 an antimicrobial peptide from i bacillus velezensis i |
| topic | peptide MS15 oxidative stress ROS (reactive oxygen species) antioxidant Nrf2/HO-1 (Nuclear factor erythroid 2-related factor-2/heme oxygenase-1) |
| url | https://www.mdpi.com/2076-3921/9/10/934 |
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