Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway
Objective Long non-coding (lnc) RNAs are critical regulators in carcinogenesis. The novel lncRNA DEPDC1 antisense RNA 1 ( DEPDC1-AS1 ) was recently associated with poor prognosis in triple-negative breast cancer and lung adenocarcinoma. However, its role in regulating the malignant progression of ga...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-04-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/03000605221093135 |
| _version_ | 1817984046029864960 |
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| author | Wei Xu Juan Wang Jinfu Xu Shenyi Li Ranran Zhang Cong Shen Min Xie Bo Zheng Menghui Gu |
| author_facet | Wei Xu Juan Wang Jinfu Xu Shenyi Li Ranran Zhang Cong Shen Min Xie Bo Zheng Menghui Gu |
| author_sort | Wei Xu |
| collection | DOAJ |
| description | Objective Long non-coding (lnc) RNAs are critical regulators in carcinogenesis. The novel lncRNA DEPDC1 antisense RNA 1 ( DEPDC1-AS1 ) was recently associated with poor prognosis in triple-negative breast cancer and lung adenocarcinoma. However, its role in regulating the malignant progression of gastric cancer (GC) and its molecular mechanism are unclear. We herein explored the functions of DEPDC1-AS1 in GC progression. Methods DEPDC1-AS1 expression and prognosis in GC tissues were examined by bioinformatics analysis and quantitative reverse transcription polymerase chain reaction. The DEPDC1-AS1 function in GC cells was explored by the cell counting kit-8 assay, colony formation assay, Transwell assay, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, 5-ethynyl-2′-deoxyuridine-incorporation, and the xenograft tumor model. The DEPDC1-AS1 and human antigen (Hu)R interaction was determined by RNA pull-down and RNA immunoprecipitation. Results DEPDC1-AS1 was overexpressed in GC tissues and cell lines, and associated with a worse prognosis in GC patients. In vitro and in vivo assays showed that DEPDC1-AS1 promoted HGC-27 cell proliferation and migration. Mechanistically, DEPDC1-AS1 served as a scaffold by combining with HuR to target the specific mRNA F11R. Conclusion DEPDC1-AS1 plays a crucial role in GC development and progression and is a potential biomarker for the early detection or prognosis of GC. |
| first_indexed | 2024-04-13T23:40:27Z |
| format | Article |
| id | doaj.art-16bb6579fa2842bbb68edb639b88a67f |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-04-13T23:40:27Z |
| publishDate | 2022-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-16bb6579fa2842bbb68edb639b88a67f2022-12-22T02:24:31ZengSAGE PublishingJournal of International Medical Research1473-23002022-04-015010.1177/03000605221093135Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathwayWei XuJuan WangJinfu XuShenyi LiRanran ZhangCong ShenMin XieBo ZhengMenghui GuObjective Long non-coding (lnc) RNAs are critical regulators in carcinogenesis. The novel lncRNA DEPDC1 antisense RNA 1 ( DEPDC1-AS1 ) was recently associated with poor prognosis in triple-negative breast cancer and lung adenocarcinoma. However, its role in regulating the malignant progression of gastric cancer (GC) and its molecular mechanism are unclear. We herein explored the functions of DEPDC1-AS1 in GC progression. Methods DEPDC1-AS1 expression and prognosis in GC tissues were examined by bioinformatics analysis and quantitative reverse transcription polymerase chain reaction. The DEPDC1-AS1 function in GC cells was explored by the cell counting kit-8 assay, colony formation assay, Transwell assay, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, 5-ethynyl-2′-deoxyuridine-incorporation, and the xenograft tumor model. The DEPDC1-AS1 and human antigen (Hu)R interaction was determined by RNA pull-down and RNA immunoprecipitation. Results DEPDC1-AS1 was overexpressed in GC tissues and cell lines, and associated with a worse prognosis in GC patients. In vitro and in vivo assays showed that DEPDC1-AS1 promoted HGC-27 cell proliferation and migration. Mechanistically, DEPDC1-AS1 served as a scaffold by combining with HuR to target the specific mRNA F11R. Conclusion DEPDC1-AS1 plays a crucial role in GC development and progression and is a potential biomarker for the early detection or prognosis of GC.https://doi.org/10.1177/03000605221093135 |
| spellingShingle | Wei Xu Juan Wang Jinfu Xu Shenyi Li Ranran Zhang Cong Shen Min Xie Bo Zheng Menghui Gu Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway Journal of International Medical Research |
| title | Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway |
| title_full | Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway |
| title_fullStr | Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway |
| title_full_unstemmed | Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway |
| title_short | Long non-coding RNA promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R–F11R pathway |
| title_sort | long non coding rna promotes proliferation and migration of human gastric cancer cells hgc 27 via the human antigen r f11r pathway |
| url | https://doi.org/10.1177/03000605221093135 |
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