Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale
Summary: Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by p...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000290 |
| Summary: | Summary: Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a “mouse pain scale.” This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex.For complete details on the use and execution of this protocol, please refer to Abdus-Saboor et al. (2019). |
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| ISSN: | 2666-1667 |