Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale
Summary: Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by p...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-03-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000290 |
| _version_ | 1818336600523800576 |
|---|---|
| author | Justin Burdge Nathan T. Fried Ishmail Abdus-Saboor |
| author_facet | Justin Burdge Nathan T. Fried Ishmail Abdus-Saboor |
| author_sort | Justin Burdge |
| collection | DOAJ |
| description | Summary: Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a “mouse pain scale.” This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex.For complete details on the use and execution of this protocol, please refer to Abdus-Saboor et al. (2019). |
| first_indexed | 2024-12-13T14:41:54Z |
| format | Article |
| id | doaj.art-16bb9289b4f5455ca0f5c9545da36169 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T14:41:54Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-16bb9289b4f5455ca0f5c9545da361692022-12-21T23:41:35ZengElsevierSTAR Protocols2666-16672021-03-0121100322Using high-speed videography for objective and reproducible pain measurement on a mouse pain scaleJustin Burdge0Nathan T. Fried1Ishmail Abdus-Saboor2University of Pennsylvania, Department of Biology, Philadelphia, PA 19104, USARutgers University, Department of Biology, Camden, NJ 08102, USAUniversity of Pennsylvania, Department of Biology, Philadelphia, PA 19104, USA; Corresponding authorSummary: Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a “mouse pain scale.” This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex.For complete details on the use and execution of this protocol, please refer to Abdus-Saboor et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166721000290Model organismsNeuroscience |
| spellingShingle | Justin Burdge Nathan T. Fried Ishmail Abdus-Saboor Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale STAR Protocols Model organisms Neuroscience |
| title | Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale |
| title_full | Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale |
| title_fullStr | Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale |
| title_full_unstemmed | Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale |
| title_short | Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale |
| title_sort | using high speed videography for objective and reproducible pain measurement on a mouse pain scale |
| topic | Model organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721000290 |
| work_keys_str_mv | AT justinburdge usinghighspeedvideographyforobjectiveandreproduciblepainmeasurementonamousepainscale AT nathantfried usinghighspeedvideographyforobjectiveandreproduciblepainmeasurementonamousepainscale AT ishmailabdussaboor usinghighspeedvideographyforobjectiveandreproduciblepainmeasurementonamousepainscale |