Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay
In order to emphasize modifications on living organisms resulted from interaction with heavy metals, we applied a simple, sensitive, quick, inexpensive and reproducible method. It evaluates modifications through determining released amino acids. This method comprises three stages: treating plants wi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Galati University Press
2014-03-01
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| Series: | The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science |
| Subjects: | |
| Online Access: | https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2647 |
| _version_ | 1819280004146528256 |
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| author | Olga PINTILIE Marius ZAHARIA Lucia TUDORACHI Sabina BĂNCILĂ Gabi DROCHIOIU Ion SANDU |
| author_facet | Olga PINTILIE Marius ZAHARIA Lucia TUDORACHI Sabina BĂNCILĂ Gabi DROCHIOIU Ion SANDU |
| author_sort | Olga PINTILIE |
| collection | DOAJ |
| description | In order to emphasize modifications on living organisms resulted from interaction with heavy metals, we applied a simple, sensitive, quick, inexpensive and reproducible method. It evaluates modifications through determining released amino acids. This method comprises three stages: treating plants with the investigated heavy metals, extracting resulted amino acids and dosing them with a ninhydrin reagent. The colored solution resulted from ninhydrin dosing is spectrophotometrically measured at 516 nm. The Lambert-Beer law is valid within the concentration field of 0-20 μg mL–1.
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| first_indexed | 2024-12-24T00:36:54Z |
| format | Article |
| id | doaj.art-16be6abecb4a404d9cd459d18a3a8bb0 |
| institution | Directory Open Access Journal |
| issn | 2668-4748 2668-4756 |
| language | English |
| last_indexed | 2024-12-24T00:36:54Z |
| publishDate | 2014-03-01 |
| publisher | Galati University Press |
| record_format | Article |
| series | The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science |
| spelling | doaj.art-16be6abecb4a404d9cd459d18a3a8bb02022-12-21T17:24:04ZengGalati University PressThe Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science2668-47482668-47562014-03-013712647Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids AssayOlga PINTILIE0Marius ZAHARIA1Lucia TUDORACHI2Sabina BĂNCILĂ3Gabi DROCHIOIU4Ion SANDU5Al. I. Cuza University of Iaşi, RomaniaAl. I. Cuza University of Iaşi, RomaniaAl. I. Cuza University of Iaşi, RomaniaAl. I. Cuza University of Iaşi, RomaniaAl. I. Cuza University of Iaşi, RomaniaAl. I. Cuza University of Iaşi, RomaniaIn order to emphasize modifications on living organisms resulted from interaction with heavy metals, we applied a simple, sensitive, quick, inexpensive and reproducible method. It evaluates modifications through determining released amino acids. This method comprises three stages: treating plants with the investigated heavy metals, extracting resulted amino acids and dosing them with a ninhydrin reagent. The colored solution resulted from ninhydrin dosing is spectrophotometrically measured at 516 nm. The Lambert-Beer law is valid within the concentration field of 0-20 μg mL–1. .https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2647biostructuredetermining amino acidshypoxiagermination testsheavy metalsninhydrin reagent |
| spellingShingle | Olga PINTILIE Marius ZAHARIA Lucia TUDORACHI Sabina BĂNCILĂ Gabi DROCHIOIU Ion SANDU Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science biostructure determining amino acids hypoxia germination tests heavy metals ninhydrin reagent |
| title | Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay |
| title_full | Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay |
| title_fullStr | Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay |
| title_full_unstemmed | Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay |
| title_short | Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay |
| title_sort | emphasizing the toxicity of heavy metal ions on plants monitoring with an amino acids assay |
| topic | biostructure determining amino acids hypoxia germination tests heavy metals ninhydrin reagent |
| url | https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2647 |
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