Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering.
In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that obtained in standard quartz...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3366972?pdf=render |
| _version_ | 1811319990084698112 |
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| author | Dominik Oberthuer Emilio Melero-García Karsten Dierks Arne Meyer Christian Betzel Alfonso Garcia-Caballero Jose A Gavira |
| author_facet | Dominik Oberthuer Emilio Melero-García Karsten Dierks Arne Meyer Christian Betzel Alfonso Garcia-Caballero Jose A Gavira |
| author_sort | Dominik Oberthuer |
| collection | DOAJ |
| description | In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that obtained in standard quartz cuvettes. Then, we have carried out DLS measurements of a counter-diffusion crystallization experiment of glucose isomerase in capillaries of different diameters (0.1, 0.2 and 0.3 mm) in order to follow the temporal evolution of protein supersaturation. Finally, we have compared DLS data with optical recordings of the progression of the crystallization front and with a simulation model of counter-diffusion in 1D. |
| first_indexed | 2024-04-13T12:51:47Z |
| format | Article |
| id | doaj.art-16c00205d49b4e508a262698d082b5df |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T12:51:47Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-16c00205d49b4e508a262698d082b5df2022-12-22T02:46:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3354510.1371/journal.pone.0033545Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering.Dominik OberthuerEmilio Melero-GarcíaKarsten DierksArne MeyerChristian BetzelAlfonso Garcia-CaballeroJose A GaviraIn this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that obtained in standard quartz cuvettes. Then, we have carried out DLS measurements of a counter-diffusion crystallization experiment of glucose isomerase in capillaries of different diameters (0.1, 0.2 and 0.3 mm) in order to follow the temporal evolution of protein supersaturation. Finally, we have compared DLS data with optical recordings of the progression of the crystallization front and with a simulation model of counter-diffusion in 1D.http://europepmc.org/articles/PMC3366972?pdf=render |
| spellingShingle | Dominik Oberthuer Emilio Melero-García Karsten Dierks Arne Meyer Christian Betzel Alfonso Garcia-Caballero Jose A Gavira Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering. PLoS ONE |
| title | Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering. |
| title_full | Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering. |
| title_fullStr | Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering. |
| title_full_unstemmed | Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering. |
| title_short | Monitoring and scoring counter-diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering. |
| title_sort | monitoring and scoring counter diffusion protein crystallization experiments in capillaries by in situ dynamic light scattering |
| url | http://europepmc.org/articles/PMC3366972?pdf=render |
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