Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients
Chronic renal allograft dysfunction (CAD) is a major limiting factor of long-term graft survival. The hallmarks of progressive CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs are small, regulatory RNAs involved in many immunological processes. In particular, microRNA-21-5p (miR-2...
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MDPI AG
2020-02-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/2/113 |
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| author | Michal S. Gniewkiewicz Izabela Paszkowska Jolanta Gozdowska Katarzyna Czerwinska Anna Sadowska-Jakubowicz Dominika Deborska-Materkowska Agnieszka Perkowska-Ptasinska Maciej Kosieradzki Magdalena Durlik |
| author_facet | Michal S. Gniewkiewicz Izabela Paszkowska Jolanta Gozdowska Katarzyna Czerwinska Anna Sadowska-Jakubowicz Dominika Deborska-Materkowska Agnieszka Perkowska-Ptasinska Maciej Kosieradzki Magdalena Durlik |
| author_sort | Michal S. Gniewkiewicz |
| collection | DOAJ |
| description | Chronic renal allograft dysfunction (CAD) is a major limiting factor of long-term graft survival. The hallmarks of progressive CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs are small, regulatory RNAs involved in many immunological processes. In particular, microRNA-21-5p (miR-21) is considered to be strongly associated with pathogenesis regarding tubulointerstitium. The aim of this study was to assess urinary miR-21 expression levels in the kidney transplant recipients and determine their application in the evaluation of IFTA and kidney allograft function. The expression levels of miR-21 were quantified in the urine of 31 kidney transplant recipients with biopsy-assessed IFTA (IFTA 0 + I: <i>n</i> = 17; IFTA II + III: <i>n</i> = 14) by real-time quantitative PCR. Urine samples were collected at the time of protocolar biopsies performed 1 or 2 years after kidney transplantation. MicroRNA-191-5p was used as reference gene. MiR-21 was significantly up-regulated in IFTA II + III group compared to IFTA 0 + I group (<i>p</i> = 0.003). MiR-21 correlated significantly with serum concentration of creatinine (r = 0.52, <i>p</i> = 0.003) and eGFR (r = −0.45; <i>p</i> = 0.01). ROC analysis determined the diagnostic value of miR-21 with an area under curve (AUC) of 0.80 (<i>p</i> = 0.0002), sensitivity of 0.86 and specificity of 0.71. miR-21 is associated with renal allograft dysfunction and IFTA. Therefore, it could be considered as a potential diagnostic, non-invasive biomarker for monitoring renal graft function. |
| first_indexed | 2024-04-11T22:10:29Z |
| format | Article |
| id | doaj.art-16c073ff9570490381904c66240d05b5 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-04-11T22:10:29Z |
| publishDate | 2020-02-01 |
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| series | Diagnostics |
| spelling | doaj.art-16c073ff9570490381904c66240d05b52022-12-22T04:00:34ZengMDPI AGDiagnostics2075-44182020-02-0110211310.3390/diagnostics10020113diagnostics10020113Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant RecipientsMichal S. Gniewkiewicz0Izabela Paszkowska1Jolanta Gozdowska2Katarzyna Czerwinska3Anna Sadowska-Jakubowicz4Dominika Deborska-Materkowska5Agnieszka Perkowska-Ptasinska6Maciej Kosieradzki7Magdalena Durlik8Department of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of General and Transplantation Surgery, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Transplantation Medicine, Nephrology and Internal Medicine, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandChronic renal allograft dysfunction (CAD) is a major limiting factor of long-term graft survival. The hallmarks of progressive CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs are small, regulatory RNAs involved in many immunological processes. In particular, microRNA-21-5p (miR-21) is considered to be strongly associated with pathogenesis regarding tubulointerstitium. The aim of this study was to assess urinary miR-21 expression levels in the kidney transplant recipients and determine their application in the evaluation of IFTA and kidney allograft function. The expression levels of miR-21 were quantified in the urine of 31 kidney transplant recipients with biopsy-assessed IFTA (IFTA 0 + I: <i>n</i> = 17; IFTA II + III: <i>n</i> = 14) by real-time quantitative PCR. Urine samples were collected at the time of protocolar biopsies performed 1 or 2 years after kidney transplantation. MicroRNA-191-5p was used as reference gene. MiR-21 was significantly up-regulated in IFTA II + III group compared to IFTA 0 + I group (<i>p</i> = 0.003). MiR-21 correlated significantly with serum concentration of creatinine (r = 0.52, <i>p</i> = 0.003) and eGFR (r = −0.45; <i>p</i> = 0.01). ROC analysis determined the diagnostic value of miR-21 with an area under curve (AUC) of 0.80 (<i>p</i> = 0.0002), sensitivity of 0.86 and specificity of 0.71. miR-21 is associated with renal allograft dysfunction and IFTA. Therefore, it could be considered as a potential diagnostic, non-invasive biomarker for monitoring renal graft function.https://www.mdpi.com/2075-4418/10/2/113iftakidney transplantationmicrornamir-21 |
| spellingShingle | Michal S. Gniewkiewicz Izabela Paszkowska Jolanta Gozdowska Katarzyna Czerwinska Anna Sadowska-Jakubowicz Dominika Deborska-Materkowska Agnieszka Perkowska-Ptasinska Maciej Kosieradzki Magdalena Durlik Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients Diagnostics ifta kidney transplantation microrna mir-21 |
| title | Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients |
| title_full | Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients |
| title_fullStr | Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients |
| title_full_unstemmed | Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients |
| title_short | Urinary MicroRNA-21-5p as Potential Biomarker of Interstitial Fibrosis and Tubular Atrophy (IFTA) in Kidney Transplant Recipients |
| title_sort | urinary microrna 21 5p as potential biomarker of interstitial fibrosis and tubular atrophy ifta in kidney transplant recipients |
| topic | ifta kidney transplantation microrna mir-21 |
| url | https://www.mdpi.com/2075-4418/10/2/113 |
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