Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining
In the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also relentlessly culture them so far as a cell stage aiming...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2015-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=306&vmd=Full |
| _version_ | 1818967131465711616 |
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| author | Kyoung Guk Kim Young Sik Park |
| author_facet | Kyoung Guk Kim Young Sik Park |
| author_sort | Kyoung Guk Kim |
| collection | DOAJ |
| description | In the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also relentlessly culture them so far as a cell stage aiming at experimental applications. For developing the efficient method to identify the stage of sperm cells, the morphological characteristics of sperm cells were investigated by staining the cells with blue fluorescent dye Hoechst 33258, and a criterion for male germ cell classification was elicited from results of the previous investigation, then the efficiency of the criterion was verified by applying it to assort the germ cells recovered from male mice in age from 6 to 35 days. As morphological characteristics, spermatogonia significantly differed from spermatocytes in size, appearance and fluorescent patches of nucleus, and spermatids could also be distinguished from spermatozoa by making a difference in the volume and shape of nucleus and the shape and fluorescence of tail. Aforesaid criterion was applicable for classifying in vitro cultured sperm cells by verifying its efficiency and propriety for assorting the stages of testicular germ cells. However, the fluorescent staining showed that germ cells in mouse testis should be dramatically differentiated and developed at 21 days and 35 days of age, which were known as times of sexual puberty and maturity in male mice, respectively. In conclusion, the results indicated that this simple criterion for sperm cell classification using fluorescence staining with Hoechst 33258 may be highly efficient and reasonable for spermatogenesis study. |
| first_indexed | 2024-12-20T13:43:55Z |
| format | Article |
| id | doaj.art-16c0e4b03cbe47ba88255dd23cb56943 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-20T13:43:55Z |
| publishDate | 2015-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-16c0e4b03cbe47ba88255dd23cb569432022-12-21T19:38:44ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632015-09-0130321321810.12750/JET.2015.30.3.213Simple Classification of Male Mouse Germ Cells using Hoechst 33258 StainingKyoung Guk Kim0Young Sik Park1Dept. of Animal Science, Agricultural College of Yanbian University, Yanji Jilin 133002, ChinaDept. of Animal Science and Biotechnology, College of Ecology and Environment Science, Kyungpook National University, Sangju 742-711, KoreaIn the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also relentlessly culture them so far as a cell stage aiming at experimental applications. For developing the efficient method to identify the stage of sperm cells, the morphological characteristics of sperm cells were investigated by staining the cells with blue fluorescent dye Hoechst 33258, and a criterion for male germ cell classification was elicited from results of the previous investigation, then the efficiency of the criterion was verified by applying it to assort the germ cells recovered from male mice in age from 6 to 35 days. As morphological characteristics, spermatogonia significantly differed from spermatocytes in size, appearance and fluorescent patches of nucleus, and spermatids could also be distinguished from spermatozoa by making a difference in the volume and shape of nucleus and the shape and fluorescence of tail. Aforesaid criterion was applicable for classifying in vitro cultured sperm cells by verifying its efficiency and propriety for assorting the stages of testicular germ cells. However, the fluorescent staining showed that germ cells in mouse testis should be dramatically differentiated and developed at 21 days and 35 days of age, which were known as times of sexual puberty and maturity in male mice, respectively. In conclusion, the results indicated that this simple criterion for sperm cell classification using fluorescence staining with Hoechst 33258 may be highly efficient and reasonable for spermatogenesis study.http://www.e-jarb.org/journal/view.html?uid=306&vmd=Fullspermatogoniain vitro spermatogenesishoechst 33258germ cell classification |
| spellingShingle | Kyoung Guk Kim Young Sik Park Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining Journal of Animal Reproduction and Biotechnology spermatogonia in vitro spermatogenesis hoechst 33258 germ cell classification |
| title | Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining |
| title_full | Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining |
| title_fullStr | Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining |
| title_full_unstemmed | Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining |
| title_short | Simple Classification of Male Mouse Germ Cells using Hoechst 33258 Staining |
| title_sort | simple classification of male mouse germ cells using hoechst 33258 staining |
| topic | spermatogonia in vitro spermatogenesis hoechst 33258 germ cell classification |
| url | http://www.e-jarb.org/journal/view.html?uid=306&vmd=Full |
| work_keys_str_mv | AT kyounggukkim simpleclassificationofmalemousegermcellsusinghoechst33258staining AT youngsikpark simpleclassificationofmalemousegermcellsusinghoechst33258staining |