Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens
A pipette-free and fully integrated device that can be used to accurately recognize the presence of infectious pathogens is an important and useful tool in point-of-care testing, particularly when aiming to decrease the unpredictable threats posed by disease outbreak. In this study, a paper device i...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/23/22/9112 |
| _version_ | 1827638742330900480 |
|---|---|
| author | Hanh An Nguyen Nae Yoon Lee |
| author_facet | Hanh An Nguyen Nae Yoon Lee |
| author_sort | Hanh An Nguyen |
| collection | DOAJ |
| description | A pipette-free and fully integrated device that can be used to accurately recognize the presence of infectious pathogens is an important and useful tool in point-of-care testing, particularly when aiming to decrease the unpredictable threats posed by disease outbreak. In this study, a paper device is developed to integrate the three main processes required for detecting infectious pathogens, including DNA extraction, loop-mediated isothermal amplification (LAMP), and detection. All key reagents, including sodium dodecyl sulfate (SDS), NaOH, LAMP reagents, and carmoisine, are placed on the paper device. The paper device is operated simply via sliding and folding without using any bulky equipment, and the results can be directly observed by the naked eye. The optimized concentrations of sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and carmoisine were found to be 0.1%, 0.1 M, and 0.5 mg/mL, respectively. The paper device was used to detect <i>Enterococcus faecium</i> at concentrations as low as 10<sup>2</sup> CFU/mL within 60 min. Also, <i>E. faecium</i> spiked in milk was successfully detected using the paper device, demonstrating the feasible application in real sample analysis. |
| first_indexed | 2024-03-09T16:28:09Z |
| format | Article |
| id | doaj.art-16c389c78e86422e99be743075f624a7 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T16:28:09Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-16c389c78e86422e99be743075f624a72023-11-24T15:05:23ZengMDPI AGSensors1424-82202023-11-012322911210.3390/s23229112Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious PathogensHanh An Nguyen0Nae Yoon Lee1Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Republic of KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Republic of KoreaA pipette-free and fully integrated device that can be used to accurately recognize the presence of infectious pathogens is an important and useful tool in point-of-care testing, particularly when aiming to decrease the unpredictable threats posed by disease outbreak. In this study, a paper device is developed to integrate the three main processes required for detecting infectious pathogens, including DNA extraction, loop-mediated isothermal amplification (LAMP), and detection. All key reagents, including sodium dodecyl sulfate (SDS), NaOH, LAMP reagents, and carmoisine, are placed on the paper device. The paper device is operated simply via sliding and folding without using any bulky equipment, and the results can be directly observed by the naked eye. The optimized concentrations of sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and carmoisine were found to be 0.1%, 0.1 M, and 0.5 mg/mL, respectively. The paper device was used to detect <i>Enterococcus faecium</i> at concentrations as low as 10<sup>2</sup> CFU/mL within 60 min. Also, <i>E. faecium</i> spiked in milk was successfully detected using the paper device, demonstrating the feasible application in real sample analysis.https://www.mdpi.com/1424-8220/23/22/9112Pipette-free and fully integrated paper devicecarmoisine-based colorimetric detection<i>Enterococcus faecium</i>point-of-care testing |
| spellingShingle | Hanh An Nguyen Nae Yoon Lee Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens Sensors Pipette-free and fully integrated paper device carmoisine-based colorimetric detection <i>Enterococcus faecium</i> point-of-care testing |
| title | Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens |
| title_full | Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens |
| title_fullStr | Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens |
| title_full_unstemmed | Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens |
| title_short | Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens |
| title_sort | pipette free and fully integrated paper device employing dna extraction isothermal amplification and carmoisine based colorimetric detection for determining infectious pathogens |
| topic | Pipette-free and fully integrated paper device carmoisine-based colorimetric detection <i>Enterococcus faecium</i> point-of-care testing |
| url | https://www.mdpi.com/1424-8220/23/22/9112 |
| work_keys_str_mv | AT hanhannguyen pipettefreeandfullyintegratedpaperdeviceemployingdnaextractionisothermalamplificationandcarmoisinebasedcolorimetricdetectionfordetermininginfectiouspathogens AT naeyoonlee pipettefreeandfullyintegratedpaperdeviceemployingdnaextractionisothermalamplificationandcarmoisinebasedcolorimetricdetectionfordetermininginfectiouspathogens |