Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002
Metabolic engineering of cyanobacteria has received much attention as a sustainable strategy to convert CO2 to various longer carbon chain fuels. Pinene has become increasingly attractive since pinene dimers contain high volumetric energy and have been proposed to act as potential aircraft fuels. Ho...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.779437/full |
| _version_ | 1819239011974119424 |
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| author | Ruigang Yang Lingyun Zhu Tao Li Lv-yun Zhu Zi Ye Dongyi Zhang |
| author_facet | Ruigang Yang Lingyun Zhu Tao Li Lv-yun Zhu Zi Ye Dongyi Zhang |
| author_sort | Ruigang Yang |
| collection | DOAJ |
| description | Metabolic engineering of cyanobacteria has received much attention as a sustainable strategy to convert CO2 to various longer carbon chain fuels. Pinene has become increasingly attractive since pinene dimers contain high volumetric energy and have been proposed to act as potential aircraft fuels. However, cyanobacteria cannot directly convert geranyl pyrophosphate into pinene due to the lack of endogenous pinene synthase. Herein, we integrated the gene encoding Abies grandis pinene synthase into the model cyanobacterium Synechococcus sp. PCC 7002 through homologous recombination. The genetically modified cyanobacteria achieved a pinene titer of 1.525 ± 0.l45 mg L−1 in the lab-scale tube photobioreactor with CO2 aeration. Specifically, the results showed a mixture of α- and β-pinene (∼33:67 ratio). The ratio of β-pinene in the product was significantly increased compared with that previously reported in the engineered Escherichia coli. Furthermore, we investigated the photoautotrophic growth performances of Synechococcus overlaid with different concentrations of dodecane. The work demonstrates that the engineered Synechococcus is a suitable potential platform for β-pinene production. |
| first_indexed | 2024-12-23T13:45:21Z |
| format | Article |
| id | doaj.art-16c4bdaa39c5423daae139a7d8263389 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-23T13:45:21Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-16c4bdaa39c5423daae139a7d82633892022-12-21T17:44:44ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-12-01910.3389/fbioe.2021.779437779437Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002Ruigang Yang0Lingyun Zhu1Tao Li2Lv-yun Zhu3Zi Ye4Dongyi Zhang5Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, ChinaDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaHunan Key Laboratory of Economic Crops, Genetic Improvement, and Integrated Utilization, School of Life Sciences, Hunan University of Science and Technology, Xiangtan, ChinaMetabolic engineering of cyanobacteria has received much attention as a sustainable strategy to convert CO2 to various longer carbon chain fuels. Pinene has become increasingly attractive since pinene dimers contain high volumetric energy and have been proposed to act as potential aircraft fuels. However, cyanobacteria cannot directly convert geranyl pyrophosphate into pinene due to the lack of endogenous pinene synthase. Herein, we integrated the gene encoding Abies grandis pinene synthase into the model cyanobacterium Synechococcus sp. PCC 7002 through homologous recombination. The genetically modified cyanobacteria achieved a pinene titer of 1.525 ± 0.l45 mg L−1 in the lab-scale tube photobioreactor with CO2 aeration. Specifically, the results showed a mixture of α- and β-pinene (∼33:67 ratio). The ratio of β-pinene in the product was significantly increased compared with that previously reported in the engineered Escherichia coli. Furthermore, we investigated the photoautotrophic growth performances of Synechococcus overlaid with different concentrations of dodecane. The work demonstrates that the engineered Synechococcus is a suitable potential platform for β-pinene production.https://www.frontiersin.org/articles/10.3389/fbioe.2021.779437/fullterpenoidspinenecyanobacteriapinene synthasedodecane |
| spellingShingle | Ruigang Yang Lingyun Zhu Tao Li Lv-yun Zhu Zi Ye Dongyi Zhang Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002 Frontiers in Bioengineering and Biotechnology terpenoids pinene cyanobacteria pinene synthase dodecane |
| title | Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002 |
| title_full | Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002 |
| title_fullStr | Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002 |
| title_full_unstemmed | Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002 |
| title_short | Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002 |
| title_sort | photosynthetic conversion of co2 into pinene using engineered synechococcus sp pcc 7002 |
| topic | terpenoids pinene cyanobacteria pinene synthase dodecane |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.779437/full |
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