Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)
Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity in two natural populations of C. amaena. A total of 50 ISSR primers were used and 13 primers producing polymorphic and reproducible pr...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2022-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2022/0354-46642200031A.pdf |
| _version_ | 1811155203494248448 |
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| author | Atasagun Bayram |
| author_facet | Atasagun Bayram |
| author_sort | Atasagun Bayram |
| collection | DOAJ |
| description | Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity in two natural populations of C. amaena. A total of 50 ISSR primers were used and 13 primers producing polymorphic and reproducible products were selected. These primers yielded 102 amplified discernible loci, of which 80 (78%) were polymorphic. A high level of genetic diversity was detected both at population and species levels; the effective number of alleles (Ne) was 1.544, the observed number of alleles (Na) was 1.784, the Nei’s genetic diversity (H) was 0.306, and Shannon’s information index was 0.447. The established gene flow (Nm) was 2.329, indicating a high migration rate between the populations. A moderate level of genetic differentiation (GST: 0.176) was also observed. Analysis of molecular variance (AMOVA) revealed that 24.89% of the total genetic diversity resided among populations, while 75.10% was within the populations. Cluster analysis showed that samples from the same locality clustered together and there was no cross-clustering between the samples. The patterns of genetic variation indicate that existing C. amaena populations should be conserved. |
| first_indexed | 2024-04-10T04:30:16Z |
| format | Article |
| id | doaj.art-16c51f205c0b4fa2a54de3cba815ed25 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-10T04:30:16Z |
| publishDate | 2022-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-16c51f205c0b4fa2a54de3cba815ed252023-03-10T08:03:10ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392022-01-0174432533210.2298/ABS220826031A0354-46642200031AAssessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)Atasagun Bayram0Vocational School of Health Services, Selçuk University, Selçuklu, Konya, TurkeyCentaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity in two natural populations of C. amaena. A total of 50 ISSR primers were used and 13 primers producing polymorphic and reproducible products were selected. These primers yielded 102 amplified discernible loci, of which 80 (78%) were polymorphic. A high level of genetic diversity was detected both at population and species levels; the effective number of alleles (Ne) was 1.544, the observed number of alleles (Na) was 1.784, the Nei’s genetic diversity (H) was 0.306, and Shannon’s information index was 0.447. The established gene flow (Nm) was 2.329, indicating a high migration rate between the populations. A moderate level of genetic differentiation (GST: 0.176) was also observed. Analysis of molecular variance (AMOVA) revealed that 24.89% of the total genetic diversity resided among populations, while 75.10% was within the populations. Cluster analysis showed that samples from the same locality clustered together and there was no cross-clustering between the samples. The patterns of genetic variation indicate that existing C. amaena populations should be conserved.http://www.doiserbia.nb.rs/img/doi/0354-4664/2022/0354-46642200031A.pdfcentaureaendemicgenetic diversityissr |
| spellingShingle | Atasagun Bayram Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae) Archives of Biological Sciences centaurea endemic genetic diversity issr |
| title | Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae) |
| title_full | Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae) |
| title_fullStr | Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae) |
| title_full_unstemmed | Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae) |
| title_short | Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae) |
| title_sort | assessment of the genetic diversity of a critically endangered species centaurea amaena asteraceae |
| topic | centaurea endemic genetic diversity issr |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2022/0354-46642200031A.pdf |
| work_keys_str_mv | AT atasagunbayram assessmentofthegeneticdiversityofacriticallyendangeredspeciescentaureaamaenaasteraceae |