Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i>
Fructan 1-exohydrolase (1-FEH) is one of the major enzymes in water-soluble carbohydrate (WSC) remobilisation for grains in wheat. We investigated the functional role of <i>1-FEH w1</i>, <i>w2</i>, and <i>w3</i> isoforms in WSC remobilisation under post-anthesis w...
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MDPI AG
2023-08-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/8/419 |
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| author | Nusrat Khan Jingjuan Zhang Shahidul Islam Rudi Appels Bernard Dell |
| author_facet | Nusrat Khan Jingjuan Zhang Shahidul Islam Rudi Appels Bernard Dell |
| author_sort | Nusrat Khan |
| collection | DOAJ |
| description | Fructan 1-exohydrolase (1-FEH) is one of the major enzymes in water-soluble carbohydrate (WSC) remobilisation for grains in wheat. We investigated the functional role of <i>1-FEH w1</i>, <i>w2</i>, and <i>w3</i> isoforms in WSC remobilisation under post-anthesis water deficit using mutation lines derived from the Australian wheat variety Chara. F1 seeds, developed by backcrossing the <i>1-FEH w1</i>, <i>w2</i>, and <i>w3</i> mutation lines with Chara, were genotyped using the Infinium 90K SNP iSelect platform to characterise the mutated region. Putative deletions were identified in <i>FEH</i> mutation lines encompassing the <i>FEH</i> genomic regions. Mapping analysis demonstrated that mutations affected significantly longer regions than the target <i>FEH</i> gene regions. Functional roles of the non-target genes were carried out utilising bioinformatics and confirmed that the non-target genes were unlikely to confound the effects considered to be due to the influence of <i>1-FEH</i> gene functions. Glasshouse experiments revealed that the <i>1-FEH w3</i> mutation line had a slower degradation and remobilisation of fructans than the <i>1-FEH w2</i> and <i>w1</i> mutation lines and Chara, which reduced grain filling and grain yield. Thus, <i>1-FEH w3</i> plays a vital role in reducing yield loss under drought. This insight into the distinct role of the <i>1-FEH</i> isoforms provides new gene targets for water-deficit-tolerant wheat breeding. |
| first_indexed | 2024-03-11T00:01:57Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T00:01:57Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-16c7307a67824d3b8881ad66e3fd8cf82023-11-19T00:41:57ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-08-014586634665010.3390/cimb45080419Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i>Nusrat Khan0Jingjuan Zhang1Shahidul Islam2Rudi Appels3Bernard Dell4Centre for Crop and Food Innovation, Food Futures Institute, Murdoch University, 90 South Street, Murdoch, WA 6163, AustraliaCentre for Crop and Food Innovation, Food Futures Institute, Murdoch University, 90 South Street, Murdoch, WA 6163, AustraliaCentre for Crop and Food Innovation, Food Futures Institute, Murdoch University, 90 South Street, Murdoch, WA 6163, AustraliaFaculty of Science, University of Melbourne, Parkville, VIC 3010, AustraliaCentre for Crop and Food Innovation, Food Futures Institute, Murdoch University, 90 South Street, Murdoch, WA 6163, AustraliaFructan 1-exohydrolase (1-FEH) is one of the major enzymes in water-soluble carbohydrate (WSC) remobilisation for grains in wheat. We investigated the functional role of <i>1-FEH w1</i>, <i>w2</i>, and <i>w3</i> isoforms in WSC remobilisation under post-anthesis water deficit using mutation lines derived from the Australian wheat variety Chara. F1 seeds, developed by backcrossing the <i>1-FEH w1</i>, <i>w2</i>, and <i>w3</i> mutation lines with Chara, were genotyped using the Infinium 90K SNP iSelect platform to characterise the mutated region. Putative deletions were identified in <i>FEH</i> mutation lines encompassing the <i>FEH</i> genomic regions. Mapping analysis demonstrated that mutations affected significantly longer regions than the target <i>FEH</i> gene regions. Functional roles of the non-target genes were carried out utilising bioinformatics and confirmed that the non-target genes were unlikely to confound the effects considered to be due to the influence of <i>1-FEH</i> gene functions. Glasshouse experiments revealed that the <i>1-FEH w3</i> mutation line had a slower degradation and remobilisation of fructans than the <i>1-FEH w2</i> and <i>w1</i> mutation lines and Chara, which reduced grain filling and grain yield. Thus, <i>1-FEH w3</i> plays a vital role in reducing yield loss under drought. This insight into the distinct role of the <i>1-FEH</i> isoforms provides new gene targets for water-deficit-tolerant wheat breeding.https://www.mdpi.com/1467-3045/45/8/419<i>1-FEH</i> isoformsgene mutationwater deficitwater-soluble carbohydratewheat |
| spellingShingle | Nusrat Khan Jingjuan Zhang Shahidul Islam Rudi Appels Bernard Dell Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i> Current Issues in Molecular Biology <i>1-FEH</i> isoforms gene mutation water deficit water-soluble carbohydrate wheat |
| title | Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i> |
| title_full | Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i> |
| title_fullStr | Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i> |
| title_full_unstemmed | Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i> |
| title_short | Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit by <i>1-FEH w3</i> |
| title_sort | wheat water soluble carbohydrate remobilisation under water deficit by i 1 feh w3 i |
| topic | <i>1-FEH</i> isoforms gene mutation water deficit water-soluble carbohydrate wheat |
| url | https://www.mdpi.com/1467-3045/45/8/419 |
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