Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties
We report the design and fabrication by laser direct writing via two photons polymerization of innovative hierarchical structures with cell-repellency capability. The structures were designed in the shape of “mushrooms”, consisting of an underside (mushroom’s leg) acting as a support structure and a...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/11/5653 |
| _version_ | 1797532574631854080 |
|---|---|
| author | Irina A. Paun Bogdan S. Calin Cosmin C. Mustaciosu Eugenia Tanasa Antoniu Moldovan Agata Niemczyk Maria Dinescu |
| author_facet | Irina A. Paun Bogdan S. Calin Cosmin C. Mustaciosu Eugenia Tanasa Antoniu Moldovan Agata Niemczyk Maria Dinescu |
| author_sort | Irina A. Paun |
| collection | DOAJ |
| description | We report the design and fabrication by laser direct writing via two photons polymerization of innovative hierarchical structures with cell-repellency capability. The structures were designed in the shape of “mushrooms”, consisting of an underside (mushroom’s leg) acting as a support structure and a top side (mushroom’s hat) decorated with micro- and nanostructures. A ripple-like pattern was created on top of the mushrooms, over length scales ranging from several µm (microstructured mushroom-like pillars, MMP) to tens of nm (nanostructured mushroom-like pillars, NMP). The MMP and NMP structures were hydrophobic, with contact angles of (127 ± 2)° and (128 ± 4)°, respectively, whereas flat polymer surfaces were hydrophilic, with a contact angle of (43 ± 1)°. The cell attachment on NMP structures was reduced by 55% as compared to the controls, whereas for the MMP, a reduction of only 21% was observed. Moreover, the MMP structures preserved the native spindle-like with phyllopodia cellular shape, whereas the cells from NMP structures showed a round shape and absence of phyllopodia. Overall, the NMP structures were more effective in impeding the cellular attachment and affected the cell shape to a greater extent than the MMP structures. The influence of the wettability on cell adhesion and shape was less important, the cellular behavior being mainly governed by structures’ topography. |
| first_indexed | 2024-03-10T11:01:10Z |
| format | Article |
| id | doaj.art-16c785e3bf4745ebb316b72bbeeb8bd5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:01:10Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-16c785e3bf4745ebb316b72bbeeb8bd52023-11-21T21:27:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211565310.3390/ijms22115653Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive PropertiesIrina A. Paun0Bogdan S. Calin1Cosmin C. Mustaciosu2Eugenia Tanasa3Antoniu Moldovan4Agata Niemczyk5Maria Dinescu6Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, RomaniaCenter for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, RomaniaHoria Hulubei National Institute for Physics and Nuclear Engineering IFIN-HH, RO-077125 Magurele-Ilfov, RomaniaFaculty of Applied Sciences, University Politehnica of Bucharest, RO-060042 Bucharest, RomaniaNational Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, RomaniaDepartment of Materials Technology, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology in Szczecin, 19 Piastow Ave, 70-310 Szczecin, PolandNational Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, RomaniaWe report the design and fabrication by laser direct writing via two photons polymerization of innovative hierarchical structures with cell-repellency capability. The structures were designed in the shape of “mushrooms”, consisting of an underside (mushroom’s leg) acting as a support structure and a top side (mushroom’s hat) decorated with micro- and nanostructures. A ripple-like pattern was created on top of the mushrooms, over length scales ranging from several µm (microstructured mushroom-like pillars, MMP) to tens of nm (nanostructured mushroom-like pillars, NMP). The MMP and NMP structures were hydrophobic, with contact angles of (127 ± 2)° and (128 ± 4)°, respectively, whereas flat polymer surfaces were hydrophilic, with a contact angle of (43 ± 1)°. The cell attachment on NMP structures was reduced by 55% as compared to the controls, whereas for the MMP, a reduction of only 21% was observed. Moreover, the MMP structures preserved the native spindle-like with phyllopodia cellular shape, whereas the cells from NMP structures showed a round shape and absence of phyllopodia. Overall, the NMP structures were more effective in impeding the cellular attachment and affected the cell shape to a greater extent than the MMP structures. The influence of the wettability on cell adhesion and shape was less important, the cellular behavior being mainly governed by structures’ topography.https://www.mdpi.com/1422-0067/22/11/5653hierarchical structureslaser direct writing via two-photon polymerizationwettabilitycell adhesion |
| spellingShingle | Irina A. Paun Bogdan S. Calin Cosmin C. Mustaciosu Eugenia Tanasa Antoniu Moldovan Agata Niemczyk Maria Dinescu Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties International Journal of Molecular Sciences hierarchical structures laser direct writing via two-photon polymerization wettability cell adhesion |
| title | Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties |
| title_full | Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties |
| title_fullStr | Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties |
| title_full_unstemmed | Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties |
| title_short | Laser Direct Writing via Two-Photon Polymerization of 3D Hierarchical Structures with Cells-Antiadhesive Properties |
| title_sort | laser direct writing via two photon polymerization of 3d hierarchical structures with cells antiadhesive properties |
| topic | hierarchical structures laser direct writing via two-photon polymerization wettability cell adhesion |
| url | https://www.mdpi.com/1422-0067/22/11/5653 |
| work_keys_str_mv | AT irinaapaun laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties AT bogdanscalin laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties AT cosmincmustaciosu laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties AT eugeniatanasa laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties AT antoniumoldovan laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties AT agataniemczyk laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties AT mariadinescu laserdirectwritingviatwophotonpolymerizationof3dhierarchicalstructureswithcellsantiadhesiveproperties |