Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics
Aquaculture is a growing sector, providing several products for human consumption, and it is therefore important to guarantee its quality and safety. This study aimed to contribute to the knowledge of bacterial composition of <i>Crassostrea gigas</i>, <i>Mytilus</i> spp. and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/9/1135 |
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| author | Vanessa Salgueiro Lígia Reis Eugénia Ferreira Maria João Botelho Vera Manageiro Manuela Caniça |
| author_facet | Vanessa Salgueiro Lígia Reis Eugénia Ferreira Maria João Botelho Vera Manageiro Manuela Caniça |
| author_sort | Vanessa Salgueiro |
| collection | DOAJ |
| description | Aquaculture is a growing sector, providing several products for human consumption, and it is therefore important to guarantee its quality and safety. This study aimed to contribute to the knowledge of bacterial composition of <i>Crassostrea gigas</i>, <i>Mytilus</i> spp. and <i>Ruditapes decussatus</i>, and the antibiotic resistances/resistance genes present in aquaculture environments. Two hundred and twenty-two bacterial strains were recovered from all bivalve mollusks samples belonging to the <i>Aeromonadaceae</i>, <i>Bacillaceae</i>, <i>Comamonadaceae</i>, <i>Enterobacteriaceae</i>, <i>Enterococcaceae</i>, <i>Micrococcaceae</i>, <i>Moraxellaceae</i>, <i>Morganellaceae</i>, <i>Pseudomonadaceae</i>, <i>Shewanellaceae</i>, <i>Staphylococcaceae</i>, <i>Streptococcaceae</i>, <i>Vibrionaceae</i>, and <i>Yersiniaceae</i> families. Decreased susceptibility to oxytetracycline prevails in all bivalve species, aquaculture farms and seasons. Decreased susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefotaxime, cefoxitin, ceftazidime, chloramphenicol, florfenicol, colistin, ciprofloxacin, flumequine, nalidixic acid and trimethoprim/sulfamethoxazole were also found. This study detected six <i>qnrA</i> genes among <i>Shewanella algae</i>, ten <i>qnrB</i> genes among <i>Citrobacter</i> spp. and <i>Escherichia coli</i>, three <i>oqxAB</i> genes from <i>Raoultella ornithinolytica</i> and <i>bla</i><sub>TEM-1</sub> in eight <i>E. coli</i> strains harboring a <i>qnrB19</i> gene. Our results suggest that the bacteria and antibiotic resistances/resistance genes present in bivalve mollusks depend on several factors, such as host species and respective life stage, bacterial family, farm’s location and season, and that is important to study each aquaculture farm individually to implement the most suitable measures to prevent outbreaks. |
| first_indexed | 2024-03-10T07:56:48Z |
| format | Article |
| id | doaj.art-16c9030e1c954788a958c208e22b2ee3 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T07:56:48Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-16c9030e1c954788a958c208e22b2ee32023-11-22T11:46:47ZengMDPI AGAntibiotics2079-63822021-09-01109113510.3390/antibiotics10091135Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to AntibioticsVanessa Salgueiro0Lígia Reis1Eugénia Ferreira2Maria João Botelho3Vera Manageiro4Manuela Caniça5National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalNational Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalNational Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalDivision of Oceanography and Marine Environment, Portuguese Institute for the Sea and Atmosphere, 1749-077 Lisbon, PortugalNational Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalNational Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalAquaculture is a growing sector, providing several products for human consumption, and it is therefore important to guarantee its quality and safety. This study aimed to contribute to the knowledge of bacterial composition of <i>Crassostrea gigas</i>, <i>Mytilus</i> spp. and <i>Ruditapes decussatus</i>, and the antibiotic resistances/resistance genes present in aquaculture environments. Two hundred and twenty-two bacterial strains were recovered from all bivalve mollusks samples belonging to the <i>Aeromonadaceae</i>, <i>Bacillaceae</i>, <i>Comamonadaceae</i>, <i>Enterobacteriaceae</i>, <i>Enterococcaceae</i>, <i>Micrococcaceae</i>, <i>Moraxellaceae</i>, <i>Morganellaceae</i>, <i>Pseudomonadaceae</i>, <i>Shewanellaceae</i>, <i>Staphylococcaceae</i>, <i>Streptococcaceae</i>, <i>Vibrionaceae</i>, and <i>Yersiniaceae</i> families. Decreased susceptibility to oxytetracycline prevails in all bivalve species, aquaculture farms and seasons. Decreased susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefotaxime, cefoxitin, ceftazidime, chloramphenicol, florfenicol, colistin, ciprofloxacin, flumequine, nalidixic acid and trimethoprim/sulfamethoxazole were also found. This study detected six <i>qnrA</i> genes among <i>Shewanella algae</i>, ten <i>qnrB</i> genes among <i>Citrobacter</i> spp. and <i>Escherichia coli</i>, three <i>oqxAB</i> genes from <i>Raoultella ornithinolytica</i> and <i>bla</i><sub>TEM-1</sub> in eight <i>E. coli</i> strains harboring a <i>qnrB19</i> gene. Our results suggest that the bacteria and antibiotic resistances/resistance genes present in bivalve mollusks depend on several factors, such as host species and respective life stage, bacterial family, farm’s location and season, and that is important to study each aquaculture farm individually to implement the most suitable measures to prevent outbreaks.https://www.mdpi.com/2079-6382/10/9/1135bivalve mollusksaquacultureantibiotic resistanceoxytetracyclinePMQR |
| spellingShingle | Vanessa Salgueiro Lígia Reis Eugénia Ferreira Maria João Botelho Vera Manageiro Manuela Caniça Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics Antibiotics bivalve mollusks aquaculture antibiotic resistance oxytetracycline PMQR |
| title | Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics |
| title_full | Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics |
| title_fullStr | Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics |
| title_full_unstemmed | Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics |
| title_short | Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics |
| title_sort | assessing the bacterial community composition of bivalve mollusks collected in aquaculture farms and respective susceptibility to antibiotics |
| topic | bivalve mollusks aquaculture antibiotic resistance oxytetracycline PMQR |
| url | https://www.mdpi.com/2079-6382/10/9/1135 |
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