Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat
The Objective of this study was to identify the existence of Campylobacter jejuni based on phenotypic and genotypic characteristic in local chicken and chicken meats. Samples of local chicken intestine and meat were tested for the bacterial existence. Phenotypic examination was carried out by mea...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science
2010-05-01
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| Series: | Animal Production: Indonesian Journal of Animal Production |
| Subjects: | |
| Online Access: | http://animalproduction.net/index.php/JAP/article/view/258 |
| _version_ | 1827297366523248640 |
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| author | A Rosyidi S Budhiharta W Asmara D Yudhabuntara |
| author_facet | A Rosyidi S Budhiharta W Asmara D Yudhabuntara |
| author_sort | A Rosyidi |
| collection | DOAJ |
| description | The Objective of this study was to identify the existence of Campylobacter jejuni based on phenotypic and genotypic characteristic in local chicken and chicken meats. Samples of local chicken intestine and meat were tested for the bacterial existence. Phenotypic examination was carried out by means of cultivation followed by gram staining and biochemical tests. Genotypic examination was conducted by polymerase chain reaction (PCR) using genus specific16S rRNA gene at 816 bp and membrane-associated protein A (mapA) gene at 589 bp as Campylobacter jejuni species-specific gene. The result of phenotypic detection revealed the existence of Campylobacter spp as gram negative, curved rod shape, oxidase positive, urease negative and motile. Genotypic examination also indicated the existence of bacteria using both primers. However, no Campylobacter jejuni detected from meat of the chickens. The results suggest that the method of PCR using a primer detecting species-specific gene of Campylobacter jejuni gives a rapid and accurate detection of the bacteria as compared to that using phenotypic and biochemical test. Identification of Campylobacter spp from chicken meats should be improved with enrichment method and sample collection. (Animal Production 12(2): 128-134 (2010)Key Words: Campylobacter jejuni, mapA gene, local chicken |
| first_indexed | 2024-04-24T14:54:15Z |
| format | Article |
| id | doaj.art-16c928396a76449facd247c03877af45 |
| institution | Directory Open Access Journal |
| issn | 1411-2027 |
| language | English |
| last_indexed | 2024-04-24T14:54:15Z |
| publishDate | 2010-05-01 |
| publisher | Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science |
| record_format | Article |
| series | Animal Production: Indonesian Journal of Animal Production |
| spelling | doaj.art-16c928396a76449facd247c03877af452024-04-02T19:27:59ZengUniversitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production: Indonesian Journal of Animal Production1411-20272010-05-01122128134Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken MeatA RosyidiS BudhihartaW AsmaraD YudhabuntaraThe Objective of this study was to identify the existence of Campylobacter jejuni based on phenotypic and genotypic characteristic in local chicken and chicken meats. Samples of local chicken intestine and meat were tested for the bacterial existence. Phenotypic examination was carried out by means of cultivation followed by gram staining and biochemical tests. Genotypic examination was conducted by polymerase chain reaction (PCR) using genus specific16S rRNA gene at 816 bp and membrane-associated protein A (mapA) gene at 589 bp as Campylobacter jejuni species-specific gene. The result of phenotypic detection revealed the existence of Campylobacter spp as gram negative, curved rod shape, oxidase positive, urease negative and motile. Genotypic examination also indicated the existence of bacteria using both primers. However, no Campylobacter jejuni detected from meat of the chickens. The results suggest that the method of PCR using a primer detecting species-specific gene of Campylobacter jejuni gives a rapid and accurate detection of the bacteria as compared to that using phenotypic and biochemical test. Identification of Campylobacter spp from chicken meats should be improved with enrichment method and sample collection. (Animal Production 12(2): 128-134 (2010)Key Words: Campylobacter jejuni, mapA gene, local chickenhttp://animalproduction.net/index.php/JAP/article/view/258Campylobacter jejunimapA genelocal chicken |
| spellingShingle | A Rosyidi S Budhiharta W Asmara D Yudhabuntara Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat Animal Production: Indonesian Journal of Animal Production Campylobacter jejuni mapA gene local chicken |
| title | Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat |
| title_full | Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat |
| title_fullStr | Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat |
| title_full_unstemmed | Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat |
| title_short | Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat |
| title_sort | phenotypic and genotypic detection of campylobacter jejuni at local chicken and chicken meat |
| topic | Campylobacter jejuni mapA gene local chicken |
| url | http://animalproduction.net/index.php/JAP/article/view/258 |
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