Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects
Background: Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO<sub>3</sub> scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with thi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/6/306 |
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| author | Leticia Faustino Adolpho Larissa Mayra Silva Ribeiro Gileade Pereira Freitas Helena Bacha Lopes Maria Paula Oliveira Gomes Emanuela Prado Ferraz Rossano Gimenes Marcio Mateus Beloti Adalberto Luiz Rosa |
| author_facet | Leticia Faustino Adolpho Larissa Mayra Silva Ribeiro Gileade Pereira Freitas Helena Bacha Lopes Maria Paula Oliveira Gomes Emanuela Prado Ferraz Rossano Gimenes Marcio Mateus Beloti Adalberto Luiz Rosa |
| author_sort | Leticia Faustino Adolpho |
| collection | DOAJ |
| description | Background: Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO<sub>3</sub> scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with this scaffold and photobiomodulation (PBM) on bone repair. Methods and results: P(VDF-TrFE)/BaTiO<sub>3</sub> was synthesized using an electrospinning technique and presented physical and chemical properties suitable for bone tissue engineering. This scaffold was implanted in rat calvarial defects (unilateral, 5 mm in diameter) and, 2 weeks post-implantation, MSCs were locally injected into these defects (<i>n</i> = 12/group). Photobiomodulation was then applied immediately, and again 48 and 96 h post-injection. The μCT and histological analyses showed an increment in bone formation, which exhibited a positive correlation with the treatments combined with the scaffold, with MSCs and PBM inducing more bone repair, followed by the scaffold combined with PBM, the scaffold combined with MSCs, and finally the scaffold alone (ANOVA, <i>p</i> ≤ 0.05). Conclusions: The P(VDF-TrFE)/BaTiO<sub>3</sub> scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects. These findings emphasize the need to combine a range of techniques to regenerate large bone defects and provide avenues for further investigations on innovative tissue engineering approaches. |
| first_indexed | 2024-03-11T02:17:02Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T02:17:02Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-16ca86218dde4e2f9b132cf5395b3ee02023-11-18T11:03:14ZengMDPI AGJournal of Functional Biomaterials2079-49832023-06-0114630610.3390/jfb14060306Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial DefectsLeticia Faustino Adolpho0Larissa Mayra Silva Ribeiro1Gileade Pereira Freitas2Helena Bacha Lopes3Maria Paula Oliveira Gomes4Emanuela Prado Ferraz5Rossano Gimenes6Marcio Mateus Beloti7Adalberto Luiz Rosa8Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilInstitute of Physics and Chemistry, University of Itajubá, Itajubá 37500-903, MG, BrazilBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilInstitute of Physics and Chemistry, University of Itajubá, Itajubá 37500-903, MG, BrazilBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, SP, BrazilBackground: Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO<sub>3</sub> scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with this scaffold and photobiomodulation (PBM) on bone repair. Methods and results: P(VDF-TrFE)/BaTiO<sub>3</sub> was synthesized using an electrospinning technique and presented physical and chemical properties suitable for bone tissue engineering. This scaffold was implanted in rat calvarial defects (unilateral, 5 mm in diameter) and, 2 weeks post-implantation, MSCs were locally injected into these defects (<i>n</i> = 12/group). Photobiomodulation was then applied immediately, and again 48 and 96 h post-injection. The μCT and histological analyses showed an increment in bone formation, which exhibited a positive correlation with the treatments combined with the scaffold, with MSCs and PBM inducing more bone repair, followed by the scaffold combined with PBM, the scaffold combined with MSCs, and finally the scaffold alone (ANOVA, <i>p</i> ≤ 0.05). Conclusions: The P(VDF-TrFE)/BaTiO<sub>3</sub> scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects. These findings emphasize the need to combine a range of techniques to regenerate large bone defects and provide avenues for further investigations on innovative tissue engineering approaches.https://www.mdpi.com/2079-4983/14/6/306bone repairelectrospinninglaser therapymesenchymal stem cellPVDF scaffold |
| spellingShingle | Leticia Faustino Adolpho Larissa Mayra Silva Ribeiro Gileade Pereira Freitas Helena Bacha Lopes Maria Paula Oliveira Gomes Emanuela Prado Ferraz Rossano Gimenes Marcio Mateus Beloti Adalberto Luiz Rosa Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects Journal of Functional Biomaterials bone repair electrospinning laser therapy mesenchymal stem cell PVDF scaffold |
| title | Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects |
| title_full | Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects |
| title_fullStr | Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects |
| title_full_unstemmed | Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects |
| title_short | Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO<sub>3</sub> Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects |
| title_sort | mesenchymal stem cells combined with a p vdf trfe batio sub 3 sub scaffold and photobiomodulation therapy enhance bone repair in rat calvarial defects |
| topic | bone repair electrospinning laser therapy mesenchymal stem cell PVDF scaffold |
| url | https://www.mdpi.com/2079-4983/14/6/306 |
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