Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals
Background: Paroxetine is one of the well-known antidepressants. Recent studies have focused on paroxetine’s probable immuno-modulatory effects, since findings have indicated inflammation’s role in the pathophysiology of depression. Therefore, in the present study, TLR2 and TLR4 mRNA genes expressio...
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| Format: | Article |
| Language: | fas |
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Tabriz University of Medical Sciences
2021-08-01
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| Series: | مجله پزشکی دانشگاه علوم پزشکی تبریز |
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| Online Access: | https://mj.tbzmed.ac.ir/PDF/mj-43-246.pdf |
| _version_ | 1828106110835359744 |
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| author | Fatemeh Ganjalishahi Anahid Hemmatpur Mahdi Dehghan manshadi Hossein Hadi nedoushan Alireza Karimollah |
| author_facet | Fatemeh Ganjalishahi Anahid Hemmatpur Mahdi Dehghan manshadi Hossein Hadi nedoushan Alireza Karimollah |
| author_sort | Fatemeh Ganjalishahi |
| collection | DOAJ |
| description | Background: Paroxetine is one of the well-known antidepressants. Recent studies have focused on paroxetine’s probable immuno-modulatory effects, since findings have indicated inflammation’s role in the pathophysiology of depression. Therefore, in the present study, TLR2 and TLR4 mRNA genes expression was assessed in paroxetine-treated peripheral blood mononuclear cells (PBMCs). Methods: Venous blood samples were drawn from five healthy men (20-40 years old). Peripheral blood mononuclear cells (PBMCs) were isolated from samples and were cultured. After the first incubation for 24h, phytohemagglutinin plus lipopolysaccharide were added to the cells and then were incubated for 24h. Thereafter, cells were treated with different concentrations of paroxetine in the presence or absence of inhibitors of 5-HT2 and 5-HT7 receptors. After incubation for 48h, RNA was extracted and cDNA was synthesized. Using the real-Time PCR technique, TLR2 and TLR4 genes mRNA expression were evaluated. Statistical analysis of data were carried out using GraphPad Prism 7. Results: TLR2 and TLR4 mRNA expression were significantly increased in response to paroxetine at all concentrations. Furthermore, the co-culture of cells with the drug and the 5-HT2R and 5HT7R inhibitor simultaneously revealed that paroxetine’s immuno-modulatory effects viaTLR2 are dependent on serotonin, while it is independent of serotonin in the case of TLR4. Conclusion: Considering paroxetine’s effect in modulating immune responses via increasing TLR2 and TLR4 expression, paroxetine could have therapeutic potentials in diseases with a deficiency in these receptors. |
| first_indexed | 2024-04-11T10:12:31Z |
| format | Article |
| id | doaj.art-16cf666ebd7b4f0f99951adc678ba1d6 |
| institution | Directory Open Access Journal |
| issn | 2783-2031 2783-204X |
| language | fas |
| last_indexed | 2024-04-11T10:12:31Z |
| publishDate | 2021-08-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | مجله پزشکی دانشگاه علوم پزشکی تبریز |
| spelling | doaj.art-16cf666ebd7b4f0f99951adc678ba1d62022-12-22T04:30:04ZfasTabriz University of Medical Sciencesمجله پزشکی دانشگاه علوم پزشکی تبریز2783-20312783-204X2021-08-0143324625310.34172/mj.2021.05027273Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individualsFatemeh Ganjalishahi0Anahid Hemmatpur1Mahdi Dehghan manshadi2Hossein Hadi nedoushan3Alireza Karimollah4Department of Pharmacology, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranReproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranReproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Pharmacology, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Paroxetine is one of the well-known antidepressants. Recent studies have focused on paroxetine’s probable immuno-modulatory effects, since findings have indicated inflammation’s role in the pathophysiology of depression. Therefore, in the present study, TLR2 and TLR4 mRNA genes expression was assessed in paroxetine-treated peripheral blood mononuclear cells (PBMCs). Methods: Venous blood samples were drawn from five healthy men (20-40 years old). Peripheral blood mononuclear cells (PBMCs) were isolated from samples and were cultured. After the first incubation for 24h, phytohemagglutinin plus lipopolysaccharide were added to the cells and then were incubated for 24h. Thereafter, cells were treated with different concentrations of paroxetine in the presence or absence of inhibitors of 5-HT2 and 5-HT7 receptors. After incubation for 48h, RNA was extracted and cDNA was synthesized. Using the real-Time PCR technique, TLR2 and TLR4 genes mRNA expression were evaluated. Statistical analysis of data were carried out using GraphPad Prism 7. Results: TLR2 and TLR4 mRNA expression were significantly increased in response to paroxetine at all concentrations. Furthermore, the co-culture of cells with the drug and the 5-HT2R and 5HT7R inhibitor simultaneously revealed that paroxetine’s immuno-modulatory effects viaTLR2 are dependent on serotonin, while it is independent of serotonin in the case of TLR4. Conclusion: Considering paroxetine’s effect in modulating immune responses via increasing TLR2 and TLR4 expression, paroxetine could have therapeutic potentials in diseases with a deficiency in these receptors.https://mj.tbzmed.ac.ir/PDF/mj-43-246.pdfparoxetinepbmctlr2tlr4 |
| spellingShingle | Fatemeh Ganjalishahi Anahid Hemmatpur Mahdi Dehghan manshadi Hossein Hadi nedoushan Alireza Karimollah Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals مجله پزشکی دانشگاه علوم پزشکی تبریز paroxetine pbmc tlr2 tlr4 |
| title | Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals |
| title_full | Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals |
| title_fullStr | Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals |
| title_full_unstemmed | Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals |
| title_short | Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals |
| title_sort | paroxetine effect on expression of tlr2 and tlr4 genes in pbmcs of mentally normal individuals |
| topic | paroxetine pbmc tlr2 tlr4 |
| url | https://mj.tbzmed.ac.ir/PDF/mj-43-246.pdf |
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