Application of Amyloid-Based Hybrid Membranes in Drug Delivery
The properties of amyloid fibrils, e.g., unique structural characteristics and superior biocompatibility, make them a promising vehicle for drug delivery. Here, carboxymethyl cellulose (CMC) and whey protein isolate amyloid fibril (WPI-AF) were used to synthesize amyloid-based hybrid membranes as ve...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/15/6/1444 |
| _version_ | 1797609323196579840 |
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| author | You-Ren Lai Steven S.-S. Wang Ti-Lun Hsu Szu-Hui Chou Su-Chun How Ta-Hsien Lin |
| author_facet | You-Ren Lai Steven S.-S. Wang Ti-Lun Hsu Szu-Hui Chou Su-Chun How Ta-Hsien Lin |
| author_sort | You-Ren Lai |
| collection | DOAJ |
| description | The properties of amyloid fibrils, e.g., unique structural characteristics and superior biocompatibility, make them a promising vehicle for drug delivery. Here, carboxymethyl cellulose (CMC) and whey protein isolate amyloid fibril (WPI-AF) were used to synthesize amyloid-based hybrid membranes as vehicles for the delivery of cationic and hydrophobic drugs (e.g., methylene blue (MB) and riboflavin (RF)). The CMC/WPI-AF membranes were synthesized via chemical crosslinking coupled with phase inversion. The zeta potential and scanning electron microscopy results revealed a negative charge and a pleated surface microstructure with a high content of WPI-AF. FTIR analysis showed that the CMC and WPI-AF were cross-linked via glutaraldehyde and the interacting forces between membrane and MB or RF was found to be electrostatic interaction and hydrogen bonding, respectively. Next, the in vitro drug release from membranes was monitored using UV-vis spectrophotometry. Additionally, two empirical models were used to analyze the drug release data and relevant rate constant and parameters were determined accordingly. Moreover, our results indicated that in vitro drug release rates depended on the drug–matrix interactions and transport mechanism, which could be controlled by altering the WPI-AF content in membrane. This research provides an excellent example of utilizing two-dimensional amyloid-based materials for drug delivery. |
| first_indexed | 2024-03-11T05:58:54Z |
| format | Article |
| id | doaj.art-16d3833f7f81400b822b7ea07cb08162 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T05:58:54Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-16d3833f7f81400b822b7ea07cb081622023-11-17T13:25:36ZengMDPI AGPolymers2073-43602023-03-01156144410.3390/polym15061444Application of Amyloid-Based Hybrid Membranes in Drug DeliveryYou-Ren Lai0Steven S.-S. Wang1Ti-Lun Hsu2Szu-Hui Chou3Su-Chun How4Ta-Hsien Lin5Department of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Chemical Engineering and Biotechnology, Tatung University, Taipei 104, TaiwanLaboratory of Nuclear Magnetic Resonance, Department of Medical Research, Taipei Veterans General Hospital, Taipei 11217, TaiwanThe properties of amyloid fibrils, e.g., unique structural characteristics and superior biocompatibility, make them a promising vehicle for drug delivery. Here, carboxymethyl cellulose (CMC) and whey protein isolate amyloid fibril (WPI-AF) were used to synthesize amyloid-based hybrid membranes as vehicles for the delivery of cationic and hydrophobic drugs (e.g., methylene blue (MB) and riboflavin (RF)). The CMC/WPI-AF membranes were synthesized via chemical crosslinking coupled with phase inversion. The zeta potential and scanning electron microscopy results revealed a negative charge and a pleated surface microstructure with a high content of WPI-AF. FTIR analysis showed that the CMC and WPI-AF were cross-linked via glutaraldehyde and the interacting forces between membrane and MB or RF was found to be electrostatic interaction and hydrogen bonding, respectively. Next, the in vitro drug release from membranes was monitored using UV-vis spectrophotometry. Additionally, two empirical models were used to analyze the drug release data and relevant rate constant and parameters were determined accordingly. Moreover, our results indicated that in vitro drug release rates depended on the drug–matrix interactions and transport mechanism, which could be controlled by altering the WPI-AF content in membrane. This research provides an excellent example of utilizing two-dimensional amyloid-based materials for drug delivery.https://www.mdpi.com/2073-4360/15/6/1444amyloidmembranedrug deliverywhey protein isolatecarboxymethyl cellulose |
| spellingShingle | You-Ren Lai Steven S.-S. Wang Ti-Lun Hsu Szu-Hui Chou Su-Chun How Ta-Hsien Lin Application of Amyloid-Based Hybrid Membranes in Drug Delivery Polymers amyloid membrane drug delivery whey protein isolate carboxymethyl cellulose |
| title | Application of Amyloid-Based Hybrid Membranes in Drug Delivery |
| title_full | Application of Amyloid-Based Hybrid Membranes in Drug Delivery |
| title_fullStr | Application of Amyloid-Based Hybrid Membranes in Drug Delivery |
| title_full_unstemmed | Application of Amyloid-Based Hybrid Membranes in Drug Delivery |
| title_short | Application of Amyloid-Based Hybrid Membranes in Drug Delivery |
| title_sort | application of amyloid based hybrid membranes in drug delivery |
| topic | amyloid membrane drug delivery whey protein isolate carboxymethyl cellulose |
| url | https://www.mdpi.com/2073-4360/15/6/1444 |
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