Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection
The instability of human serum albumin (HSA) in urine samples makes fresh urine a requirement for microalbumin analyses using immunoturbidimetry. Here, we determined the ability of an aptasensor-based fluorescent platform to detect microalbumin in old, boric acid-preserved urine samples. Our results...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/915 |
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| author | Chalermwoot Sompark Wireeya Chawjiraphan Manatsaphon Sukmak Ubon Cha’on Sirirat Anutrakulchai Prapasiri Pongprayoon Thitirat Putnin Dechnarong Pimalai Visarute Pinrod Deanpen Japrung |
| author_facet | Chalermwoot Sompark Wireeya Chawjiraphan Manatsaphon Sukmak Ubon Cha’on Sirirat Anutrakulchai Prapasiri Pongprayoon Thitirat Putnin Dechnarong Pimalai Visarute Pinrod Deanpen Japrung |
| author_sort | Chalermwoot Sompark |
| collection | DOAJ |
| description | The instability of human serum albumin (HSA) in urine samples makes fresh urine a requirement for microalbumin analyses using immunoturbidimetry. Here, we determined the ability of an aptasensor-based fluorescent platform to detect microalbumin in old, boric acid-preserved urine samples. Our results show that the cleavage site of protease enzymes on urine albumin protein differed from the binding position of the aptamer on HSA protein, suggesting the aptasensor may be effective for albumin detection in non-fresh urine. Furthermore, the addition of boric acid in urine samples over a short term (at ambient temperature (T<sub>a</sub>) and 4 °C), long term (−20 and −80 °C), and following freeze–thawing (1–3 cycles) did not significantly affect albumin stability, as analyzed using the aptasensor. Therefore, boric acid stabilized has in urine stored over a short- and long-term. Thus, the aptasensor developed by us is applicable for HSA detection in boric acid-preserved urine that has been stored for 7-d at T<sub>a</sub> and 4 °C, and in the long-term at −80 °C. |
| first_indexed | 2024-03-09T19:13:53Z |
| format | Article |
| id | doaj.art-16d5be781a054fd5ab822ce854acddfe |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T19:13:53Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-16d5be781a054fd5ab822ce854acddfe2023-11-24T03:54:22ZengMDPI AGBiosensors2079-63742022-10-01121191510.3390/bios12110915Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent DetectionChalermwoot Sompark0Wireeya Chawjiraphan1Manatsaphon Sukmak2Ubon Cha’on3Sirirat Anutrakulchai4Prapasiri Pongprayoon5Thitirat Putnin6Dechnarong Pimalai7Visarute Pinrod8Deanpen Japrung9National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, ThailandDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandChronic Kidney Disease Prevention in the Northeast of Thailand (CKDNET), Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Science, Department of Chemistry, Kasetsart University, Chatuchak, Bangkok 10900, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, ThailandThe instability of human serum albumin (HSA) in urine samples makes fresh urine a requirement for microalbumin analyses using immunoturbidimetry. Here, we determined the ability of an aptasensor-based fluorescent platform to detect microalbumin in old, boric acid-preserved urine samples. Our results show that the cleavage site of protease enzymes on urine albumin protein differed from the binding position of the aptamer on HSA protein, suggesting the aptasensor may be effective for albumin detection in non-fresh urine. Furthermore, the addition of boric acid in urine samples over a short term (at ambient temperature (T<sub>a</sub>) and 4 °C), long term (−20 and −80 °C), and following freeze–thawing (1–3 cycles) did not significantly affect albumin stability, as analyzed using the aptasensor. Therefore, boric acid stabilized has in urine stored over a short- and long-term. Thus, the aptasensor developed by us is applicable for HSA detection in boric acid-preserved urine that has been stored for 7-d at T<sub>a</sub> and 4 °C, and in the long-term at −80 °C.https://www.mdpi.com/2079-6374/12/11/915boric acidstorage temperaturemicroalbumingraphene oxide-based fluorescent detection |
| spellingShingle | Chalermwoot Sompark Wireeya Chawjiraphan Manatsaphon Sukmak Ubon Cha’on Sirirat Anutrakulchai Prapasiri Pongprayoon Thitirat Putnin Dechnarong Pimalai Visarute Pinrod Deanpen Japrung Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection Biosensors boric acid storage temperature microalbumin graphene oxide-based fluorescent detection |
| title | Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection |
| title_full | Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection |
| title_fullStr | Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection |
| title_full_unstemmed | Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection |
| title_short | Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection |
| title_sort | effects of boric acid and storage temperature on the analysis of microalbumin using aptasensor based fluorescent detection |
| topic | boric acid storage temperature microalbumin graphene oxide-based fluorescent detection |
| url | https://www.mdpi.com/2079-6374/12/11/915 |
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