A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts
We test a newly developed instrument prototype which utilizes time-resolved chlorophyll-a fluorescence techniques and fluctuating light to characterize Symbiodiniaceae functional traits across seven different coral species under cultivation as part of ongoing restoration efforts in the Florida Keys....
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1092202/full |
| _version_ | 1811161600790364160 |
|---|---|
| author | Kenneth D. Hoadley Kenneth D. Hoadley Grant Lockridge Audrey McQuagge Audrey McQuagge K. Blue Pahl K. Blue Pahl Sean Lowry Sean Lowry Sophie Wong Sophie Wong Sophie Wong Zachary Craig Chelsea Petrik Courtney Klepac Erinn M. Muller |
| author_facet | Kenneth D. Hoadley Kenneth D. Hoadley Grant Lockridge Audrey McQuagge Audrey McQuagge K. Blue Pahl K. Blue Pahl Sean Lowry Sean Lowry Sophie Wong Sophie Wong Sophie Wong Zachary Craig Chelsea Petrik Courtney Klepac Erinn M. Muller |
| author_sort | Kenneth D. Hoadley |
| collection | DOAJ |
| description | We test a newly developed instrument prototype which utilizes time-resolved chlorophyll-a fluorescence techniques and fluctuating light to characterize Symbiodiniaceae functional traits across seven different coral species under cultivation as part of ongoing restoration efforts in the Florida Keys. While traditional chlorophyll-a fluorescence techniques only provide a handful of algal biometrics, the system and protocol we have developed generates > 1000 dynamic measurements in a short (~11 min) time frame. Resulting ‘high-content’ algal biometric data revealed distinct phenotypes, which broadly corresponded to genus-level Symbiodiniaceae designations determined using quantitative PCR. Next, algal biometric data from Acropora cervicornis (10 genotypes) and A. palmata (5 genotypes) coral fragments was correlated with bleaching response metrics collected after a two month-long exposure to high temperature. A network analysis identified 1973 correlations (Spearman R > 0.5) between algal biometrics and various bleaching response metrics. These identified biomarkers of thermal stress were then utilized to train a predictive model, and when tested against the same A. cervicornis and A. palmata coral fragments, yielded high correlation (R = 0.92) with measured thermal response (reductions in absorbance by chlorophyll-a). When applied to all seven coral species, the model ranked fragments dominated by Cladocopium or Breviolum symbionts as more bleaching susceptible than corals harboring thermally tolerant symbionts (Durusdinium). While direct testing of bleaching predictions on novel genotypes is still needed, our device and modeling pipeline may help broaden the scalability of existing approaches for determining thermal tolerance in reef corals. Our instrument prototype and analytical pipeline aligns with recent coral restoration assessments that call for the development of novel tools for improving scalability of coral restoration programs. |
| first_indexed | 2024-04-10T06:16:58Z |
| format | Article |
| id | doaj.art-16d7ffdb84a64442843244e73312aab7 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-10T06:16:58Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-16d7ffdb84a64442843244e73312aab72023-03-02T05:50:22ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-03-011010.3389/fmars.2023.10922021092202A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbiontsKenneth D. Hoadley0Kenneth D. Hoadley1Grant Lockridge2Audrey McQuagge3Audrey McQuagge4K. Blue Pahl5K. Blue Pahl6Sean Lowry7Sean Lowry8Sophie Wong9Sophie Wong10Sophie Wong11Zachary Craig12Chelsea Petrik13Courtney Klepac14Erinn M. Muller15Dept of Biological Sciences, University of Alabama, Tuscaloosa AL, United StatesDauphin Island Sea Lab, Dauphin Island AL, United StatesDauphin Island Sea Lab, Dauphin Island AL, United StatesDept of Biological Sciences, University of Alabama, Tuscaloosa AL, United StatesDauphin Island Sea Lab, Dauphin Island AL, United StatesDept of Biological Sciences, University of Alabama, Tuscaloosa AL, United StatesDauphin Island Sea Lab, Dauphin Island AL, United StatesDept of Biological Sciences, University of Alabama, Tuscaloosa AL, United StatesDauphin Island Sea Lab, Dauphin Island AL, United StatesDept of Biological Sciences, University of Alabama, Tuscaloosa AL, United StatesDauphin Island Sea Lab, Dauphin Island AL, United StatesBiology, University of Virginia, Charlottesville, VA, United StatesMote Marine Laboratory, Summerland Key, FL, United StatesMote Marine Laboratory, Summerland Key, FL, United StatesMote Marine Laboratory, Summerland Key, FL, United StatesMote Marine Laboratory, Sarasota, FL, United StatesWe test a newly developed instrument prototype which utilizes time-resolved chlorophyll-a fluorescence techniques and fluctuating light to characterize Symbiodiniaceae functional traits across seven different coral species under cultivation as part of ongoing restoration efforts in the Florida Keys. While traditional chlorophyll-a fluorescence techniques only provide a handful of algal biometrics, the system and protocol we have developed generates > 1000 dynamic measurements in a short (~11 min) time frame. Resulting ‘high-content’ algal biometric data revealed distinct phenotypes, which broadly corresponded to genus-level Symbiodiniaceae designations determined using quantitative PCR. Next, algal biometric data from Acropora cervicornis (10 genotypes) and A. palmata (5 genotypes) coral fragments was correlated with bleaching response metrics collected after a two month-long exposure to high temperature. A network analysis identified 1973 correlations (Spearman R > 0.5) between algal biometrics and various bleaching response metrics. These identified biomarkers of thermal stress were then utilized to train a predictive model, and when tested against the same A. cervicornis and A. palmata coral fragments, yielded high correlation (R = 0.92) with measured thermal response (reductions in absorbance by chlorophyll-a). When applied to all seven coral species, the model ranked fragments dominated by Cladocopium or Breviolum symbionts as more bleaching susceptible than corals harboring thermally tolerant symbionts (Durusdinium). While direct testing of bleaching predictions on novel genotypes is still needed, our device and modeling pipeline may help broaden the scalability of existing approaches for determining thermal tolerance in reef corals. Our instrument prototype and analytical pipeline aligns with recent coral restoration assessments that call for the development of novel tools for improving scalability of coral restoration programs.https://www.frontiersin.org/articles/10.3389/fmars.2023.1092202/fullcoral bleachingalgal phenotypingSymbiodiniaceaephotobiologychlorophyll a fluorescence |
| spellingShingle | Kenneth D. Hoadley Kenneth D. Hoadley Grant Lockridge Audrey McQuagge Audrey McQuagge K. Blue Pahl K. Blue Pahl Sean Lowry Sean Lowry Sophie Wong Sophie Wong Sophie Wong Zachary Craig Chelsea Petrik Courtney Klepac Erinn M. Muller A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts Frontiers in Marine Science coral bleaching algal phenotyping Symbiodiniaceae photobiology chlorophyll a fluorescence |
| title | A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts |
| title_full | A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts |
| title_fullStr | A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts |
| title_full_unstemmed | A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts |
| title_short | A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts |
| title_sort | phenomic modeling approach for using chlorophyll a fluorescence based measurements on coral photosymbionts |
| topic | coral bleaching algal phenotyping Symbiodiniaceae photobiology chlorophyll a fluorescence |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1092202/full |
| work_keys_str_mv | AT kennethdhoadley aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kennethdhoadley aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT grantlockridge aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT audreymcquagge aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT audreymcquagge aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kbluepahl aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kbluepahl aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT seanlowry aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT seanlowry aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT sophiewong aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT sophiewong aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT sophiewong aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT zacharycraig aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT chelseapetrik aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT courtneyklepac aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT erinnmmuller aphenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kennethdhoadley phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kennethdhoadley phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT grantlockridge phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT audreymcquagge phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT audreymcquagge phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kbluepahl phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT kbluepahl phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT seanlowry phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT seanlowry phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT sophiewong phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT sophiewong phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT sophiewong phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT zacharycraig phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT chelseapetrik phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT courtneyklepac phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts AT erinnmmuller phenomicmodelingapproachforusingchlorophyllafluorescencebasedmeasurementsoncoralphotosymbionts |