Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate
Peripheral nerve injuries cause different degrees of nerve palsy and function loss. Due to the limitations of autografts, nerve tissue engineering (TE) scaffolds incorporated with various neurotrophic factors and cells have been investigated to promote nerve regeneration. However, the molecular mech...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/2/84 |
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| author | Yuye Huang Kailei Xu Jingyi Liu Guangli Dai Jun Yin Peng Wei |
| author_facet | Yuye Huang Kailei Xu Jingyi Liu Guangli Dai Jun Yin Peng Wei |
| author_sort | Yuye Huang |
| collection | DOAJ |
| description | Peripheral nerve injuries cause different degrees of nerve palsy and function loss. Due to the limitations of autografts, nerve tissue engineering (TE) scaffolds incorporated with various neurotrophic factors and cells have been investigated to promote nerve regeneration. However, the molecular mechanism is still poorly understood. In this study, we co-cultured Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on 50% degrees of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. The SCs were encapsulated within the GelMA, and PC-12 cells were on the surface. A 5% GelMA was used as the co-culture scaffold since it better supports SCs proliferation, viability, and myelination and promotes higher neurotrophic factors secretion than 10% GelMA. In the co-culture, PC-12 cells demonstrated a higher cell proliferation rate and axonal extension than culturing without SCs, indicating that the secretion of neurotrophic factors from SCs can stimulate PC-12 growth and axonal outgrowth. The mRNA level for neurotrophic factors of SCs in 5% GelMA was further evaluated. We found significant upregulation when compared with a 2D culture, which suggested that this co-culture system could be a potential scaffold to investigate the mechanism of how SCs affect neuronal behaviors. |
| first_indexed | 2024-03-09T21:53:30Z |
| format | Article |
| id | doaj.art-16dc07d9dba849e78510f0301ecf13ff |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T21:53:30Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-16dc07d9dba849e78510f0301ecf13ff2023-11-23T20:01:31ZengMDPI AGGels2310-28612022-01-01828410.3390/gels8020084Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate SubstrateYuye Huang0Kailei Xu1Jingyi Liu2Guangli Dai3Jun Yin4Peng Wei5Department of Plastic and Reconstructive Surgery, Ningbo First Hospital, Ningbo 315010, ChinaDepartment of Plastic and Reconstructive Surgery, Ningbo First Hospital, Ningbo 315010, ChinaThe State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, ChinaDepartment of Medical Engineering, Ningbo First Hospital, Ningbo 315010, ChinaThe State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310028, ChinaDepartment of Plastic and Reconstructive Surgery, Ningbo First Hospital, Ningbo 315010, ChinaPeripheral nerve injuries cause different degrees of nerve palsy and function loss. Due to the limitations of autografts, nerve tissue engineering (TE) scaffolds incorporated with various neurotrophic factors and cells have been investigated to promote nerve regeneration. However, the molecular mechanism is still poorly understood. In this study, we co-cultured Schwann cells (SCs) and rat adrenal pheochromocytoma (PC-12) cells on 50% degrees of methacryloyl substitution gelatin methacrylate (GelMA) scaffold. The SCs were encapsulated within the GelMA, and PC-12 cells were on the surface. A 5% GelMA was used as the co-culture scaffold since it better supports SCs proliferation, viability, and myelination and promotes higher neurotrophic factors secretion than 10% GelMA. In the co-culture, PC-12 cells demonstrated a higher cell proliferation rate and axonal extension than culturing without SCs, indicating that the secretion of neurotrophic factors from SCs can stimulate PC-12 growth and axonal outgrowth. The mRNA level for neurotrophic factors of SCs in 5% GelMA was further evaluated. We found significant upregulation when compared with a 2D culture, which suggested that this co-culture system could be a potential scaffold to investigate the mechanism of how SCs affect neuronal behaviors.https://www.mdpi.com/2310-2861/8/2/84gelatin methacrylate3D cultureSchwann cellsneurotrophic factorsnerve regeneration |
| spellingShingle | Yuye Huang Kailei Xu Jingyi Liu Guangli Dai Jun Yin Peng Wei Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate Gels gelatin methacrylate 3D culture Schwann cells neurotrophic factors nerve regeneration |
| title | Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate |
| title_full | Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate |
| title_fullStr | Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate |
| title_full_unstemmed | Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate |
| title_short | Promotion of Adrenal Pheochromocytoma (PC-12) Cell Proliferation and Outgrowth Using Schwann Cell-Laden Gelatin Methacrylate Substrate |
| title_sort | promotion of adrenal pheochromocytoma pc 12 cell proliferation and outgrowth using schwann cell laden gelatin methacrylate substrate |
| topic | gelatin methacrylate 3D culture Schwann cells neurotrophic factors nerve regeneration |
| url | https://www.mdpi.com/2310-2861/8/2/84 |
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