Neuronal Localization of SENP Proteins with Super Resolution Microscopy
SUMOylation of proteins plays a key role in modulating neuronal function. For this reason, the balance between protein SUMOylation and deSUMOylation requires fine regulation to guarantee the homeostasis of neural tissue. While extensive research has been carried out on the localization and function...
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MDPI AG
2020-10-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/10/11/778 |
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| author | Luca Colnaghi Andrea Conz Luca Russo Clara A. Musi Luana Fioriti Tiziana Borsello Mario Salmona |
| author_facet | Luca Colnaghi Andrea Conz Luca Russo Clara A. Musi Luana Fioriti Tiziana Borsello Mario Salmona |
| author_sort | Luca Colnaghi |
| collection | DOAJ |
| description | SUMOylation of proteins plays a key role in modulating neuronal function. For this reason, the balance between protein SUMOylation and deSUMOylation requires fine regulation to guarantee the homeostasis of neural tissue. While extensive research has been carried out on the localization and function of small ubiquitin-related modifier (SUMO) variants in neurons, less attention has been paid to the SUMO-specific isopeptidases that constitute the human SUMO-specific isopeptidase (SENP)/Ubiquitin-Specific Protease (ULP) cysteine protease family (SENP1-3 and SENP5-7). Here, for the first time, we studied the localization of SENP1, SENP6, and SENP7 in cultured hippocampal primary neurons at a super resolution detail level, with structured illumination microscopy (SIM). We found that the deSUMOylases partially colocalize with pre- and post-synaptic markers such as synaptophysin and drebrin. Thus, further confirming the presence with synaptic markers of the negative regulators of the SUMOylation machinery. |
| first_indexed | 2024-03-10T15:20:25Z |
| format | Article |
| id | doaj.art-16de6f15b1ca4f6e95aa6492abc7d1f6 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-10T15:20:25Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-16de6f15b1ca4f6e95aa6492abc7d1f62023-11-20T18:28:07ZengMDPI AGBrain Sciences2076-34252020-10-01101177810.3390/brainsci10110778Neuronal Localization of SENP Proteins with Super Resolution MicroscopyLuca Colnaghi0Andrea Conz1Luca Russo2Clara A. Musi3Luana Fioriti4Tiziana Borsello5Mario Salmona6Department of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalyDepartment of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalyDepartment of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalyDepartment of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalyDepartment of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalyDepartment of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalyDepartment of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 20156 Milan, ItalySUMOylation of proteins plays a key role in modulating neuronal function. For this reason, the balance between protein SUMOylation and deSUMOylation requires fine regulation to guarantee the homeostasis of neural tissue. While extensive research has been carried out on the localization and function of small ubiquitin-related modifier (SUMO) variants in neurons, less attention has been paid to the SUMO-specific isopeptidases that constitute the human SUMO-specific isopeptidase (SENP)/Ubiquitin-Specific Protease (ULP) cysteine protease family (SENP1-3 and SENP5-7). Here, for the first time, we studied the localization of SENP1, SENP6, and SENP7 in cultured hippocampal primary neurons at a super resolution detail level, with structured illumination microscopy (SIM). We found that the deSUMOylases partially colocalize with pre- and post-synaptic markers such as synaptophysin and drebrin. Thus, further confirming the presence with synaptic markers of the negative regulators of the SUMOylation machinery.https://www.mdpi.com/2076-3425/10/11/778SUMOSENPsneuronssuper resolution microscopy |
| spellingShingle | Luca Colnaghi Andrea Conz Luca Russo Clara A. Musi Luana Fioriti Tiziana Borsello Mario Salmona Neuronal Localization of SENP Proteins with Super Resolution Microscopy Brain Sciences SUMO SENPs neurons super resolution microscopy |
| title | Neuronal Localization of SENP Proteins with Super Resolution Microscopy |
| title_full | Neuronal Localization of SENP Proteins with Super Resolution Microscopy |
| title_fullStr | Neuronal Localization of SENP Proteins with Super Resolution Microscopy |
| title_full_unstemmed | Neuronal Localization of SENP Proteins with Super Resolution Microscopy |
| title_short | Neuronal Localization of SENP Proteins with Super Resolution Microscopy |
| title_sort | neuronal localization of senp proteins with super resolution microscopy |
| topic | SUMO SENPs neurons super resolution microscopy |
| url | https://www.mdpi.com/2076-3425/10/11/778 |
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