Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy
AIM: To investigate a possible trigeminal proprioceptive-oculomotor neural pathway and explore possible synaptic connections between neurons in this pathway. Attempt to bring a new insight to mechanism of Marcus Gunn syndrome (MGS). METHODS: Anterograde and retrograde tract tracing was applied and...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-03-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ijo.cn/en_publish/2018/3/20180306.pdf |
| _version_ | 1828395705420480512 |
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| author | Hou-Cheng Liang Jing-Dong Zhang Pi-Fu Luo Ying Qiao An-Le Su Ting Zhang Hong-Na Zhu |
| author_facet | Hou-Cheng Liang Jing-Dong Zhang Pi-Fu Luo Ying Qiao An-Le Su Ting Zhang Hong-Na Zhu |
| author_sort | Hou-Cheng Liang |
| collection | DOAJ |
| description | AIM: To investigate a possible trigeminal proprioceptive-oculomotor neural pathway and explore possible synaptic connections between neurons in this pathway. Attempt to bring a new insight to mechanism of Marcus Gunn syndrome (MGS).
METHODS: Anterograde and retrograde tract tracing was applied and combined with immunofluorescent stain in rats. After electrophysiological identifying mesencephalic trigeminal nucleus (Vme) neurons, intracellular injection of tracer was performed to trace axon trajectory.
RESULTS: Following injections of anterograde tracers into the Vme, labeled terminals were observed ipsilateral in oculomotor and trochlear nuclei (III/IV), as well as in their premotor neurons in interstitial nucleus of Cajal and Darkschewitsch nucleus (INC/DN). Combining with choline acetyltransferase (ChAT) immunofluorescent stain, it showed that Vme projecting terminals contact upon ChAT positive III/IV motoneurons under confocal microscope. By retrograde labeling premotor neurons of the III, it showed that Vme neuronal terminals contact with retrogradely labeled pre-oculomotor neurons in the INC/DN. Axons of intracellularly labeled Vme neurons that respond to electric stimuli of the masseter nerve traveled into the ipsilateral III.
CONCLUSION: There may exist a trigeminal proprioceptive- oculomotor system neural circuit in the rat, which is probably related to vertical-torsional eye movements. Possible association of this pathway with MGS etiology was discussed. |
| first_indexed | 2024-12-10T08:17:37Z |
| format | Article |
| id | doaj.art-16dfda4f936444c2815d10bf642b5caa |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-10T08:17:37Z |
| publishDate | 2018-03-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-16dfda4f936444c2815d10bf642b5caa2022-12-22T01:56:25ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-03-0111338238810.18240/ijo.2018.03.06Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomyHou-Cheng Liang0Jing-Dong Zhang1Pi-Fu Luo2Ying Qiao3An-Le Su4Ting Zhang5Hong-Na Zhu6Xi’an BRIGHT Eye Hospital, Xi’an 710068, Shaanxi Province, China; Department of Ophthalmology, the Second Affiliated Hospital of Xi’an Jiaotong University (Xibei Hospital), Xi’an 710002, Shaanxi Province, China; Shaanxi Provincial Eye Research Institute and Xi’an Eye Hospital, Xi’an 710002, Shaanxi Province, ChinaXi’an BRIGHT Eye Hospital, Xi’an 710068, Shaanxi Province, China; Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati 45267, OH, USADepartment of Pathology, King Med Diagnostics, International Bioisland, Guangzhou 510050, Guangdong Province, ChinaShaanxi Provincial Eye Research Institute and Xi’an Eye Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Provincial Eye Research Institute and Xi’an Eye Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Provincial Eye Research Institute and Xi’an Eye Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Provincial Eye Research Institute and Xi’an Eye Hospital, Xi’an 710002, Shaanxi Province, ChinaAIM: To investigate a possible trigeminal proprioceptive-oculomotor neural pathway and explore possible synaptic connections between neurons in this pathway. Attempt to bring a new insight to mechanism of Marcus Gunn syndrome (MGS). METHODS: Anterograde and retrograde tract tracing was applied and combined with immunofluorescent stain in rats. After electrophysiological identifying mesencephalic trigeminal nucleus (Vme) neurons, intracellular injection of tracer was performed to trace axon trajectory. RESULTS: Following injections of anterograde tracers into the Vme, labeled terminals were observed ipsilateral in oculomotor and trochlear nuclei (III/IV), as well as in their premotor neurons in interstitial nucleus of Cajal and Darkschewitsch nucleus (INC/DN). Combining with choline acetyltransferase (ChAT) immunofluorescent stain, it showed that Vme projecting terminals contact upon ChAT positive III/IV motoneurons under confocal microscope. By retrograde labeling premotor neurons of the III, it showed that Vme neuronal terminals contact with retrogradely labeled pre-oculomotor neurons in the INC/DN. Axons of intracellularly labeled Vme neurons that respond to electric stimuli of the masseter nerve traveled into the ipsilateral III. CONCLUSION: There may exist a trigeminal proprioceptive- oculomotor system neural circuit in the rat, which is probably related to vertical-torsional eye movements. Possible association of this pathway with MGS etiology was discussed.http://www.ijo.cn/en_publish/2018/3/20180306.pdf388mesencephalic trigeminal nucleusoculomotor and trochlear nucleiinterstitial nucleus of Cajal and Darkschewitsch nucleusanterograde and retrograde tract tracingintracellular tract tracing |
| spellingShingle | Hou-Cheng Liang Jing-Dong Zhang Pi-Fu Luo Ying Qiao An-Le Su Ting Zhang Hong-Na Zhu Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy International Journal of Ophthalmology 388 mesencephalic trigeminal nucleus oculomotor and trochlear nuclei interstitial nucleus of Cajal and Darkschewitsch nucleus anterograde and retrograde tract tracing intracellular tract tracing |
| title | Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy |
| title_full | Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy |
| title_fullStr | Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy |
| title_full_unstemmed | Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy |
| title_short | Is Marcus Gunn jaw winking a primitive reflex? Rat neuroanatomy |
| title_sort | is marcus gunn jaw winking a primitive reflex rat neuroanatomy |
| topic | 388 mesencephalic trigeminal nucleus oculomotor and trochlear nuclei interstitial nucleus of Cajal and Darkschewitsch nucleus anterograde and retrograde tract tracing intracellular tract tracing |
| url | http://www.ijo.cn/en_publish/2018/3/20180306.pdf |
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